OCA at 16:27, 29 November 2023

OCA — Wed, 29 Nov 2023 16:27:32 GMT

←Older revision		Revision as of 16:27, 29 November 2023
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	==Crystal structure of Oryza sativa Os4BGlu18 monolignol beta-glucosidase==		==Crystal structure of Oryza sativa Os4BGlu18 monolignol beta-glucosidase==
-	<StructureSection load='7d6a' size='340' side='right'caption='[[7d6a]]' scene=''>	+	<StructureSection load='7d6a' size='340' side='right'caption='[[7d6a]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D6A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7D6A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7d6a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D6A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D6A FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7d6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d6a OCA], [~~http~~://pdbe.org/7d6a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7d6a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7d6a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7d6a ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d6a OCA], [https://pdbe.org/7d6a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d6a RCSB], [https://www.ebi.ac.uk/pdbsum/7d6a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d6a ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BGL18_ORYSJ BGL18_ORYSJ] Hydrolyzes glycosides and monolignol glucosides (PubMed:25219312). Can hydrolyze para-nitrophenyl beta-D-glucopyranoside (pNPGlc) in vitro (PubMed:23811195, PubMed:25219312). Hydrolyzes para-nitrophenyl beta-D-fucopyranoside, para-nitrophenyl beta-D-galactopyranoside and para-nitrophenyl beta-D-xylopyranoside in vitro (PubMed:25219312). Hydrolyzes the monolignol glucosides coniferin and syringin with high catalytic efficiencies (PubMed:25219312).<ref>PMID:23811195</ref> <ref>PMID:25219312</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monolignol glucosides are storage forms of monolignols, which are polymerized to lignin to strengthen plant cell walls. The conversion of monolignol glucosides to monolignols is catalyzed by monolignol beta-glucosidases. Rice Os4BGlu18 beta-glucosidase catalyzes hydrolysis of the monolignol glucosides, coniferin, syringin, and p-coumaryl alcohol glucoside more efficiently than other natural substrates. To understand more clearly the basis for substrate specificity of a monolignol beta-glucosidase, the structure of Os4BGlu18 was determined by X-ray crystallography. Crystals of Os4BGlu18 and its complex with delta-gluconolactone diffracted to 1.7 and 2.1 A resolution, respectively. Two protein molecules were found in the asymmetric unit of the P212121 space group of their isomorphous crystals. The Os4BGlu18 structure exhibited the typical (beta/alpha)8 TIM barrel of glycoside hydrolase family 1 (GH1), but the four variable loops and two disulfide bonds appeared significantly different from other known structures of GH1 beta-glucosidases. Molecular docking studies of the Os4BGlu18 structure with monolignol substrate ligands placed the glycone in a similar position to the delta-gluconolactone in the complex structure and revealed the interactions between protein and ligands. Molecular docking, multiple sequence alignment, and homology modeling identified amino acid residues at the aglycone-binding site involved in substrate specificity for monolignol beta-glucosides. Thus, the structural basis of substrate recognition and hydrolysis by monolignol beta-glucosidases was elucidated.
		+
		+	Structural analysis of rice Os4BGlu18 monolignol beta-glucosidase.,Baiya S, Pengthaisong S, Kitjaruwankul S, Ketudat Cairns JR PLoS One. 2021 Jan 20;16(1):e0241325. doi: 10.1371/journal.pone.0241325. , eCollection 2021. PMID:33471829<ref>PMID:33471829</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7d6a" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-glucosidase 3D structures\|Beta-glucosidase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Oryza sativa Japonica Group]]
	[[Category: Baiya S]]		[[Category: Baiya S]]
	[[Category: Ketudat Cairns JR]]		[[Category: Ketudat Cairns JR]]
	[[Category: Pengthaisong S]]		[[Category: Pengthaisong S]]

OCA at 05:55, 20 January 2021

OCA — Wed, 20 Jan 2021 05:55:35 GMT

←Older revision		Revision as of 05:55, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7d6a is ~~ON HOLD~~	+	==Crystal structure of Oryza sativa Os4BGlu18 monolignol beta-glucosidase==
-		+	<StructureSection load='7d6a' size='340' side='right'caption='[[7d6a]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D6A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7D6A FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7d6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d6a OCA], [http://pdbe.org/7d6a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7d6a RCSB], [http://www.ebi.ac.uk/pdbsum/7d6a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7d6a ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Baiya S]]
		+	[[Category: Ketudat Cairns JR]]
		+	[[Category: Pengthaisong S]]

OCA: Protected "7d6a" [edit=sysop:move=sysop]

OCA — Wed, 07 Oct 2020 04:38:28 GMT

Protected "7d6a" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:38, 7 October 2020

OCA: New page: '''Unreleased structure''' The entry 7d6a is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Oct 2020 04:38:27 GMT

New page: '''Unreleased structure''' The entry 7d6a is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7d6a is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7d6a - Revision history

OCA at 16:27, 29 November 2023

OCA at 05:55, 20 January 2021

OCA: Protected "7d6a" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7d6a is ON HOLD Authors: Description: Category: Unreleased Structures