7d84 - Revision history

OCA at 10:53, 27 March 2024

OCA — Wed, 27 Mar 2024 10:53:15 GMT

←Older revision		Revision as of 10:53, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='7d84' size='340' side='right'caption='[[7d84]], [[Resolution\|resolution]] 3.70Å' scene=''>		<StructureSection load='7d84' size='340' side='right'caption='[[7d84]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7d84]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7d84]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~fliF~~, ~~fla AII.1, fla BI, STM1969 (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.7Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY]] The M ring may be actively involved in energy transduction.	+	[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY] The M ring may be actively involved in energy transduction.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The bacterial flagellar MS ring is a transmembrane complex acting as the core of the flagellar motor and template for flagellar assembly. The C ring attached to the MS ring is involved in torque generation and rotation switch, and a large symmetry mismatch between these two rings has been a long puzzle, especially with respect to their role in motor function. Here, using cryoEM structural analysis of the flagellar basal body and the MS ring formed by full-length FliF from Salmonella enterica, we show that the native MS ring is formed by 34 FliF subunits with no symmetry variation. Symmetry analysis of the C ring shows a variation with a peak at 34-fold, suggesting flexibility in C ring assembly. Finally, our data also indicate that FliF subunits assume two different conformations, contributing differentially to the inner and middle parts of the M ring and thus resulting in 23- and 11-fold subsymmetries in the inner and middle M ring, respectively. The internal core of the M ring, formed by 23 subunits, forms a hole of the right size to accommodate the protein export gate.	+
-		+
-	Native flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries.,Kawamoto A, Miyata T, Makino F, Kinoshita M, Minamino T, Imada K, Kato T, Namba K Nat Commun. 2021 Jul 9;12(1):4223. doi: 10.1038/s41467-021-24507-9. PMID:34244518<ref>PMID:34244518</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7d84" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Imada, K]]	+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-	[[Category: Kato, T]]	+	[[Category: Imada K]]
-	[[Category: Kawamoto, A]]	+	[[Category: Kato T]]
-	[[Category: Kinoshita, M]]	+	[[Category: Kawamoto A]]
-	[[Category: Makino, F]]	+	[[Category: Kinoshita M]]
-	[[Category: Minamino, T]]	+	[[Category: Makino F]]
-	[[Category: Miyata, T]]	+	[[Category: Minamino T]]
-	[[Category: Namba, K]]	+	[[Category: Miyata T]]
-	~~[[Category: Flagellar motor]]~~	+	[[Category: Namba K]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Torque generation]]~~	+
-	~~[[Category: Transmembrane protein complex]]~~	+
-	~~[[Category: Type iii protein export system~~]]	+

OCA at 10:47, 28 July 2021

OCA — Wed, 28 Jul 2021 10:47:31 GMT

←Older revision		Revision as of 10:47, 28 July 2021
Line 1:		Line 1:

	==34-fold symmetry Salmonella S ring formed by full-length FliF==		==34-fold symmetry Salmonella S ring formed by full-length FliF==
-	<StructureSection load='7d84' size='340' side='right'caption='[[7d84]]' scene=''>	+	<StructureSection load='7d84' size='340' side='right'caption='[[7d84]], [[Resolution\|resolution]] 3.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7d84]] is a 34 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fliF, fla AII.1, fla BI, STM1969 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FLIF_SALTY FLIF_SALTY]] The M ring may be actively involved in energy transduction.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial flagellar MS ring is a transmembrane complex acting as the core of the flagellar motor and template for flagellar assembly. The C ring attached to the MS ring is involved in torque generation and rotation switch, and a large symmetry mismatch between these two rings has been a long puzzle, especially with respect to their role in motor function. Here, using cryoEM structural analysis of the flagellar basal body and the MS ring formed by full-length FliF from Salmonella enterica, we show that the native MS ring is formed by 34 FliF subunits with no symmetry variation. Symmetry analysis of the C ring shows a variation with a peak at 34-fold, suggesting flexibility in C ring assembly. Finally, our data also indicate that FliF subunits assume two different conformations, contributing differentially to the inner and middle parts of the M ring and thus resulting in 23- and 11-fold subsymmetries in the inner and middle M ring, respectively. The internal core of the M ring, formed by 23 subunits, forms a hole of the right size to accommodate the protein export gate.
		+
		+	Native flagellar MS ring is formed by 34 subunits with 23-fold and 11-fold subsymmetries.,Kawamoto A, Miyata T, Makino F, Kinoshita M, Minamino T, Imada K, Kato T, Namba K Nat Commun. 2021 Jul 9;12(1):4223. doi: 10.1038/s41467-021-24507-9. PMID:34244518<ref>PMID:34244518</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7d84" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Imada K]]	+	[[Category: Imada, K]]
-	[[Category: Kato T]]	+	[[Category: Kato, T]]
-	[[Category: Kawamoto A]]	+	[[Category: Kawamoto, A]]
-	[[Category: Kinoshita M]]	+	[[Category: Kinoshita, M]]
-	[[Category: Makino F]]	+	[[Category: Makino, F]]
-	[[Category: Minamino T]]	+	[[Category: Minamino, T]]
-	[[Category: Miyata T]]	+	[[Category: Miyata, T]]
-	[[Category: Namba K]]	+	[[Category: Namba, K]]
		+	[[Category: Flagellar motor]]
		+	[[Category: Membrane protein]]
		+	[[Category: Torque generation]]
		+	[[Category: Transmembrane protein complex]]
		+	[[Category: Type iii protein export system]]

OCA at 10:08, 19 May 2021

OCA — Wed, 19 May 2021 10:08:59 GMT

←Older revision		Revision as of 10:08, 19 May 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7d84 is ~~ON HOLD until Paper Publication~~	+	==34-fold symmetry Salmonella S ring formed by full-length FliF==
-		+	<StructureSection load='7d84' size='340' side='right'caption='[[7d84]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D84 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d84 OCA], [https://pdbe.org/7d84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d84 RCSB], [https://www.ebi.ac.uk/pdbsum/7d84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d84 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Imada K]]
		+	[[Category: Kato T]]
		+	[[Category: Kawamoto A]]
		+	[[Category: Kinoshita M]]
		+	[[Category: Makino F]]
		+	[[Category: Minamino T]]
		+	[[Category: Miyata T]]
		+	[[Category: Namba K]]

OCA at 05:27, 4 November 2020

OCA — Wed, 04 Nov 2020 05:27:20 GMT

←Older revision		Revision as of 05:27, 4 November 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7d84 is ON HOLD	+	The entry 7d84 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7d84" [edit=sysop:move=sysop]

OCA — Wed, 14 Oct 2020 04:13:00 GMT

Protected "7d84" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:13, 14 October 2020

OCA: New page: '''Unreleased structure''' The entry 7d84 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Oct 2020 04:12:59 GMT

New page: '''Unreleased structure''' The entry 7d84 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7d84 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]