7d8o - Revision history

OCA at 16:28, 29 November 2023

OCA — Wed, 29 Nov 2023 16:28:32 GMT

←Older revision		Revision as of 16:28, 29 November 2023
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-	====	+	==Crystal structure of E. coli ToxIN type III toxin-antitoxin complex==
-	<StructureSection load='7d8o' size='340' side='right'caption='[[7d8o]]' scene=''>	+	<StructureSection load='7d8o' size='340' side='right'caption='[[7d8o]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7d8o]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D8O FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d8o OCA], [https://pdbe.org/7d8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d8o RCSB], [https://www.ebi.ac.uk/pdbsum/7d8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d8o ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.097Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d8o OCA], [https://pdbe.org/7d8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d8o RCSB], [https://www.ebi.ac.uk/pdbsum/7d8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d8o ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Toxin-antitoxin (TA) systems are proposed to play crucial roles in bacterial growth under stress conditions such as phage infection. The type III TA systems consist of a protein toxin whose activity is inhibited by a noncoding RNA antitoxin. The toxin is an endoribonuclease, while the antitoxin consists of multiple repeats of RNA. The toxin assembles with the individual antitoxin repeats into a cyclic complex in which the antitoxin forms a pseudoknot structure. While structure and functions of some type III TA systems are characterized, the complex assembly process is not well understood. Using bioinformatics analysis, we have identified type III TA systems belonging to the ToxIN family across different Escherichia coli strains and found them to be clustered into at least five distinct clusters. Furthermore, we report a 2.097 A resolution crystal structure of the first E. coli ToxIN complex that revealed the overall assembly of the protein-RNA complex. Isothermal titration calorimetry experiments showed that toxin forms a high-affinity complex with antitoxin RNA resulting from two independent (5' and 3' sides of RNA) RNA binding sites on the protein. These results further our understanding of the assembly of type III TA complexes in bacteria.
		+
		+	Identification, functional characterization, assembly and structure of ToxIN type III toxin-antitoxin complex from E. coli.,Manikandan P, Sandhya S, Nadig K, Paul S, Srinivasan N, Rothweiler U, Singh M Nucleic Acids Res. 2022 Feb 22;50(3):1687-1700. doi: 10.1093/nar/gkab1264. PMID:35018473<ref>PMID:35018473</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7d8o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Manikandan P]]
		+	[[Category: Rothweiler U]]
		+	[[Category: Singh M]]

OCA at 10:39, 5 January 2022

OCA — Wed, 05 Jan 2022 10:39:36 GMT

←Older revision		Revision as of 10:39, 5 January 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7d8o ~~is ON HOLD until Apr 08 2023~~	+	====
-		+	<StructureSection load='7d8o' size='340' side='right'caption='[[7d8o]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d8o OCA], [https://pdbe.org/7d8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d8o RCSB], [https://www.ebi.ac.uk/pdbsum/7d8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d8o ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:23, 22 September 2021

OCA — Wed, 22 Sep 2021 05:23:35 GMT

←Older revision		Revision as of 05:23, 22 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7d8o is ON HOLD until ~~Paper Publication~~	+	The entry 7d8o is ON HOLD until Apr 08 2023

	Authors:		Authors:

OCA at 07:59, 18 November 2020

OCA — Wed, 18 Nov 2020 07:59:45 GMT

←Older revision		Revision as of 07:59, 18 November 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7d8o is ON HOLD	+	The entry 7d8o is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7d8o" [edit=sysop:move=sysop]

OCA — Wed, 14 Oct 2020 04:14:07 GMT

Protected "7d8o" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:14, 14 October 2020

OCA: New page: '''Unreleased structure''' The entry 7d8o is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Oct 2020 04:14:06 GMT

New page: '''Unreleased structure''' The entry 7d8o is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7d8o is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]