7dak - Revision history

OCA at 16:29, 29 November 2023

OCA — Wed, 29 Nov 2023 16:29:37 GMT

←Older revision		Revision as of 16:29, 29 November 2023
Line 1:		Line 1:

	==The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa==		==The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa==
-	<StructureSection load='7dak' size='340' side='right'caption='[[7dak]]' scene=''>	+	<StructureSection load='7dak' size='340' side='right'caption='[[7dak]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DAK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dak]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DAK FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dak OCA], [https://pdbe.org/7dak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dak RCSB], [https://www.ebi.ac.uk/pdbsum/7dak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dak ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=F5U:2-(5-methoxy-1H-indol-3-yl)ethanamine'>F5U</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dak OCA], [https://pdbe.org/7dak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dak RCSB], [https://www.ebi.ac.uk/pdbsum/7dak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dak ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SNAT1_ORYSJ SNAT1_ORYSJ] Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin (PubMed:22998587, PubMed:24134674, PubMed:27121038). Catalyzes in vitro the N-acetylation of tryptamine to produce N-acetyltryptamine, 5-methoxytryptamine to produce melatonin and tyramine to produce N-acetyltyramine (PubMed:27121038). Acetyltransferase required for geminivirus infection and systemic spread (By similarity).[UniProtKB:Q7X9V3]<ref>PMID:22998587</ref> <ref>PMID:24134674</ref> <ref>PMID:27121038</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serotonin N-acetyltransferase (SNAT) is the key rate-limiting enzyme in melatonin biosynthesis. It mediates melatonin biosynthesis in plants by using serotonin and 5-methoxytryptamine (5-MT), but little is known of its underlying mechanisms. Herein, we present a detailed reaction mechanism of a SNAT from Oryza sativa through combined structural and molecular dynamics (MD) analysis. We report the crystal structures of plant SNAT in the apo and binary/ternary complex forms with acetyl-CoA (AcCoA), serotonin, and 5-MT. OsSNAT exhibits a unique enzymatically active dimeric fold not found in the known structures of arylalkylamine N-acetyltransferase (AANAT) family. The key residues W188, D189, D226, N220, and Y233 located around the active pocket are important in catalysis, confirmed by site-directed mutagenesis. Combined with MD simulations, we hypothesize a novel plausible catalytic mechanism in which D226 and Y233 function as catalytic base and acid during the acetyl-transfer reaction.
		+
		+	Structural and Molecular Dynamics Analysis of Plant Serotonin N-Acetyltransferase Reveal an Acid/Base-Assisted Catalysis in Melatonin Biosynthesis.,Liao L, Zhou Y, Xu Y, Zhang Y, Liu X, Liu B, Chen X, Guo Y, Zeng Z, Zhao Y Angew Chem Int Ed Engl. 2021 May 17;60(21):12020-12026. doi:, 10.1002/anie.202100992. Epub 2021 Apr 7. PMID:33682300<ref>PMID:33682300</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7dak" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serotonin N-acetyltransferase\|Serotonin N-acetyltransferase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Oryza sativa Japonica Group]]
	[[Category: Guo Y]]		[[Category: Guo Y]]
	[[Category: Liao LJ]]		[[Category: Liao LJ]]

OCA at 06:49, 22 September 2021

OCA — Wed, 22 Sep 2021 06:49:12 GMT

←Older revision		Revision as of 06:49, 22 September 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7dak is ON HOLD until Paper Publication~~	+	==The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa==
-		+	<StructureSection load='7dak' size='340' side='right'caption='[[7dak]]' scene=''>
-	~~Authors: Zhou, Y.Z., Liao, L.J., Tang, T., Guo, Y., Liu, X.K., Liu, B., Zhao, Y.C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DAK FirstGlance]. <br>
-	~~Description:~~ The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dak OCA], [https://pdbe.org/7dak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dak RCSB], [https://www.ebi.ac.uk/pdbsum/7dak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dak ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Liao~~, L.J]]	+	__TOC__
-	[[Category: ~~Zhou, Y.Z~~]]	+	</StructureSection>
-	[[Category: Guo, Y]]	+	[[Category: Large Structures]]
-	[[Category: ~~Zhao, Y.C~~]]	+	[[Category: Guo Y]]
-	[[Category: Tang, T]]	+	[[Category: Liao LJ]]
-	[[Category: ~~Liu, X.K~~]]	+	[[Category: Liu B]]
-	[[Category: ~~Liu, B~~]]	+	[[Category: Liu XK]]
		+	[[Category: Tang T]]
		+	[[Category: Zhao YC]]
		+	[[Category: Zhou YZ]]

OCA at 11:04, 16 December 2020

OCA — Wed, 16 Dec 2020 11:04:44 GMT

←Older revision		Revision as of 11:04, 16 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7dak is ON HOLD	+	The entry 7dak is ON HOLD until Paper Publication

	Authors: Zhou, Y.Z., Liao, L.J., Tang, T., Guo, Y., Liu, X.K., Liu, B., Zhao, Y.C.		Authors: Zhou, Y.Z., Liao, L.J., Tang, T., Guo, Y., Liu, X.K., Liu, B., Zhao, Y.C.
Line 7:		Line 7:
	Description: The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa		Description: The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Liu~~, X.~~K]]~~	+	[[Category: Liao, L.J]]
-	~~[[Category: Zhao, Y.C]]~~	+
-	~~[[Category: Tang, T~~]]	+
	[[Category: Zhou, Y.Z]]		[[Category: Zhou, Y.Z]]
	[[Category: Guo, Y]]		[[Category: Guo, Y]]
		+	[[Category: Zhao, Y.C]]
		+	[[Category: Tang, T]]
		+	[[Category: Liu, X.K]]
	[[Category: Liu, B]]		[[Category: Liu, B]]
-	[[Category: Liao, L.J]]

OCA: Protected "7dak" [edit=sysop:move=sysop]

OCA — Wed, 28 Oct 2020 19:02:12 GMT

Protected "7dak" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:02, 28 October 2020

OCA: New page: '''Unreleased structure''' The entry 7dak is ON HOLD Authors: Zhou, Y.Z., Liao, L.J., Tang, T., Guo, Y., Liu, X.K., Liu, B., Zhao, Y.C. Description: The crystal structure of a serotoni...

OCA — Wed, 28 Oct 2020 19:02:11 GMT

New page: '''Unreleased structure''' The entry 7dak is ON HOLD Authors: Zhou, Y.Z., Liao, L.J., Tang, T., Guo, Y., Liu, X.K., Liu, B., Zhao, Y.C. Description: The crystal structure of a serotoni...

New page

'''Unreleased structure'''

The entry 7dak is ON HOLD

Authors: Zhou, Y.Z., Liao, L.J., Tang, T., Guo, Y., Liu, X.K., Liu, B., Zhao, Y.C.

Description: The crystal structure of a serotonin N-acetyltransferase in complex with 5-Methoxytryptamine and acetyl-CoA from Oryza Sativa
[[Category: Unreleased Structures]]
[[Category: Liu, X.K]]
[[Category: Zhao, Y.C]]
[[Category: Tang, T]]
[[Category: Zhou, Y.Z]]
[[Category: Guo, Y]]
[[Category: Liu, B]]
[[Category: Liao, L.J]]