7dc0 - Revision history

OCA at 16:30, 29 November 2023

OCA — Wed, 29 Nov 2023 16:30:26 GMT

←Older revision		Revision as of 16:30, 29 November 2023
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	<StructureSection load='7dc0' size='340' side='right'caption='[[7dc0]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='7dc0' size='340' side='right'caption='[[7dc0]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7dc0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pseudomonas_taiwanensis_dsm_21245~~ Pseudomonas taiwanensis ~~dsm~~ 21245]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DC0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dc0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_taiwanensis_DSM_21245 Pseudomonas taiwanensis DSM 21245]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DC0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~PTL ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1292030 Pseudomonas taiwanensis DSM 21245])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dc0 OCA], [https://pdbe.org/7dc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7dc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dc0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dc0 OCA], [https://pdbe.org/7dc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7dc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dc0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2L1HSZ4_9PSED A0A2L1HSZ4_9PSED]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pseudomonas taiwanensis ~~dsm~~ 21245]]	+	[[Category: Pseudomonas taiwanensis DSM 21245]]
-	[[Category: Matoba, Y]]	+	[[Category: Matoba Y]]
-	[[Category: Oda, K]]	+	[[Category: Oda K]]
-	~~[[Category: Antiviral lectin]]~~	+
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Carbohydrate binding]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 09:27, 26 May 2021

OCA — Wed, 26 May 2021 09:27:19 GMT

←Older revision		Revision as of 09:27, 26 May 2021
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	==Crystal structure of glycan-free Pseudomonas taiwanensis lectin==		==Crystal structure of glycan-free Pseudomonas taiwanensis lectin==
-	<StructureSection load='7dc0' size='340' side='right'caption='[[7dc0]]' scene=''>	+	<StructureSection load='7dc0' size='340' side='right'caption='[[7dc0]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DC0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dc0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_taiwanensis_dsm_21245 Pseudomonas taiwanensis dsm 21245]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DC0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dc0 OCA], [https://pdbe.org/7dc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7dc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dc0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PTL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1292030 Pseudomonas taiwanensis DSM 21245])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dc0 OCA], [https://pdbe.org/7dc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7dc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dc0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Homologues of the Oscillatoria agardhii agglutinin (OAA) lectins contain a sequence repeat of approximately 66 amino acids, with the number of tandem repeats varying across family members. OAA homologues bind high-mannose glycans on viral surface proteins, thereby interfering with viral entry into host cells. As such, OAA homologues have potential utility as antiviral agents, but a more detailed understanding of their structure-function relationships would enable us to develop improved constructs. Here, we determined the X-ray crystal structure of free and glycan-bound forms of Pseudomonas taiwanensis lectin (PTL), an OAA-family lectin consisting of two tandem repeats. Like other OAA-family lectins, PTL exhibited a beta-barrel-like structure with two symmetrically positioned glycan-binding sites at the opposite ends of the barrel. Upon glycan binding, the conformation of PTL undergoes a more significant change than expected from previous OAA structural analysis. Moreover, the electron density of the bound glycans suggested that the binding affinities are different at the two binding sites. Next, based on analysis of these structures, we used site-specific mutagenesis to create PTL constructs expected to increase the population with a conformation suitable for glycan binding. The engineered PTLs were examined for their antiviral activity against the influenza virus. Interestingly, some exhibited stronger activity compared with that of the parent PTL. We propose that our approach is effective for the generation of potential microbicides with enhanced antiviral activity.
		+
		+	Lectins engineered to favor a glycan-binding conformation have enhanced antiviral activity.,Matoba Y, Sato Y, Oda K, Hatori Y, Morimoto K J Biol Chem. 2021 Apr 22:100698. doi: 10.1016/j.jbc.2021.100698. PMID:33895142<ref>PMID:33895142</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7dc0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Matoba Y]]	+	[[Category: Pseudomonas taiwanensis dsm 21245]]
-	[[Category: Oda K]]	+	[[Category: Matoba, Y]]
		+	[[Category: Oda, K]]
		+	[[Category: Antiviral lectin]]
		+	[[Category: Beta barrel]]
		+	[[Category: Carbohydrate binding]]
		+	[[Category: Sugar binding protein]]

OCA at 05:21, 28 April 2021

OCA — Wed, 28 Apr 2021 05:21:05 GMT

←Older revision		Revision as of 05:21, 28 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7dc0 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of glycan-free Pseudomonas taiwanensis lectin==
-		+	<StructureSection load='7dc0' size='340' side='right'caption='[[7dc0]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DC0 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dc0 OCA], [https://pdbe.org/7dc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7dc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dc0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Matoba Y]]
		+	[[Category: Oda K]]

OCA at 06:24, 11 November 2020

OCA — Wed, 11 Nov 2020 06:24:33 GMT

←Older revision		Revision as of 06:24, 11 November 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7dc0 is ON HOLD	+	The entry 7dc0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7dc0" [edit=sysop:move=sysop]

OCA — Wed, 28 Oct 2020 19:05:00 GMT

Protected "7dc0" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:05, 28 October 2020

OCA: New page: '''Unreleased structure''' The entry 7dc0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Oct 2020 19:04:58 GMT

New page: '''Unreleased structure''' The entry 7dc0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7dc0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]