7djc - Revision history

OCA at 16:34, 29 November 2023

OCA — Wed, 29 Nov 2023 16:34:18 GMT

←Older revision		Revision as of 16:34, 29 November 2023
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	<StructureSection load='7djc' size='340' side='right'caption='[[7djc]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='7djc' size='340' side='right'caption='[[7djc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7djc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex~~ aeolicus~~" huber and stetter 2001~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7djc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BOG:B~~-~~OCTYLGLUCOSIDE'>BOG</scene>~~, ~~<scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.701Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height~~: ~~3em;~~'>~~[[7dii\|7dii]]~~, ~~[[7dix\|7dix]], [[7dj1\|7dj1]], [[7dj2\|7dj2]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djc OCA], [https://pdbe.org/7djc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djc RCSB], [https://www.ebi.ac.uk/pdbsum/7djc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djc OCA], [https://pdbe.org/7djc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djc RCSB], [https://www.ebi.ac.uk/pdbsum/7djc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O67854_AQUAE O67854_AQUAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7djc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7djc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Symporter 3D structures\|Symporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aquifex aeolicus ~~huber and stetter 2001~~]]	+	[[Category: Aquifex aeolicus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fan, J]]	+	[[Category: Fan J]]
-	[[Category: Sun, Z]]	+	[[Category: Sun Z]]
-	[[Category: Xiao, Y]]	+	[[Category: Xiao Y]]
-	[[Category: Zhou, X]]	+	[[Category: Zhou X]]
-	~~[[Category: Neurotransmitter transporter]]~~	+
-	~~[[Category: Nss]]~~	+
-	~~[[Category: Slc6]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 08:33, 21 April 2021

OCA — Wed, 21 Apr 2021 08:33:20 GMT

←Older revision		Revision as of 08:33, 21 April 2021
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	==Crystal structure of the G26C/Q250A mutant of LeuT==		==Crystal structure of the G26C/Q250A mutant of LeuT==
-	<StructureSection load='7djc' size='340' side='right'caption='[[7djc]]' scene=''>	+	<StructureSection load='7djc' size='340' side='right'caption='[[7djc]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7djc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djc OCA], [https://pdbe.org/7djc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djc RCSB], [https://www.ebi.ac.uk/pdbsum/7djc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djc ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7dii\|7dii]], [[7dix\|7dix]], [[7dj1\|7dj1]], [[7dj2\|7dj2]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djc OCA], [https://pdbe.org/7djc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djc RCSB], [https://www.ebi.ac.uk/pdbsum/7djc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djc ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The neurotransmitter:sodium symporter (NSS) homolog LeuT from Aquifex aeolicus has proven to be a valuable model for studying the transport mechanism of the NSS family. Crystal structures have captured LeuT in key conformations visited during the transport cycle, allowing for the construction of a nearly complete model of transport, with much of the conformational dynamics studied by computational simulations. Here, we report crystal structures of LeuT representing new intermediate conformations between the outward-facing open and occluded states. These structures, combined with binding and accessibility studies, reveal details of conformational dynamics that can follow substrate binding at the central substrate-binding site (S1) of LeuT in outward-facing states, suggesting a potential competition for direction between the outward-open and outward-occluded states at this stage during substrate transport. Our structures further support an intimate interplay between the protonation state of Glu290 and binding of Na1 that may ultimately regulate the outward-open-to-occluded transition.
		+
		+	Crystal structures of LeuT reveal conformational dynamics in the outward-facing states.,Fan J, Xiao Y, Quick M, Yang Y, Sun Z, Javitch JA, Zhou X J Biol Chem. 2021 Apr 1:100609. doi: 10.1016/j.jbc.2021.100609. PMID:33811858<ref>PMID:33811858</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7djc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aquifex aeolicus huber and stetter 2001]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fan J]]	+	[[Category: Fan, J]]
-	[[Category: Sun Z]]	+	[[Category: Sun, Z]]
-	[[Category: Xiao Y]]	+	[[Category: Xiao, Y]]
-	[[Category: Zhou X]]	+	[[Category: Zhou, X]]
		+	[[Category: Neurotransmitter transporter]]
		+	[[Category: Nss]]
		+	[[Category: Slc6]]
		+	[[Category: Transport protein]]

OCA at 06:51, 7 April 2021

OCA — Wed, 07 Apr 2021 06:51:00 GMT

←Older revision		Revision as of 06:51, 7 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7djc ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the G26C/Q250A mutant of LeuT==
-		+	<StructureSection load='7djc' size='340' side='right'caption='[[7djc]]' scene=''>
-	~~Authors~~: ~~Fan~~, J., ~~Xiao~~, Y., ~~Sun, Z~~., ~~Zhou, X~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DJC FirstGlance]. <br>
-	~~Description: Crystal structure of the G26C~~/~~Q250A mutant of LeuT~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7djc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7djc OCA], [https://pdbe.org/7djc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7djc RCSB], [https://www.ebi.ac.uk/pdbsum/7djc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7djc ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Zhou, X~~]]	+	__TOC__
-	[[Category: ~~Xiao, Y~~]]	+	</StructureSection>
-	[[Category: ~~Fan, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Sun, Z~~]]	+	[[Category: Fan J]]
		+	[[Category: Sun Z]]
		+	[[Category: Xiao Y]]
		+	[[Category: Zhou X]]

OCA at 08:35, 17 February 2021

OCA — Wed, 17 Feb 2021 08:35:27 GMT

←Older revision		Revision as of 08:35, 17 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7djc is ON HOLD	+	The entry 7djc is ON HOLD until Paper Publication

	Authors: Fan, J., Xiao, Y., Sun, Z., Zhou, X.		Authors: Fan, J., Xiao, Y., Sun, Z., Zhou, X.
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	Description: Crystal structure of the G26C/Q250A mutant of LeuT		Description: Crystal structure of the G26C/Q250A mutant of LeuT
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Zhou, X]]
	[[Category: Xiao, Y]]		[[Category: Xiao, Y]]
	[[Category: Fan, J]]		[[Category: Fan, J]]
-	[[Category: Zhou, X]]
	[[Category: Sun, Z]]		[[Category: Sun, Z]]

OCA: Protected "7djc" [edit=sysop:move=sysop]

OCA — Wed, 25 Nov 2020 05:27:23 GMT

Protected "7djc" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:27, 25 November 2020

OCA: New page: '''Unreleased structure''' The entry 7djc is ON HOLD Authors: Fan, J., Xiao, Y., Sun, Z., Zhou, X. Description: Crystal structure of the G26C/Q250A mutant of LeuT [[Category: Unrelease...

OCA — Wed, 25 Nov 2020 05:27:21 GMT

New page: '''Unreleased structure''' The entry 7djc is ON HOLD Authors: Fan, J., Xiao, Y., Sun, Z., Zhou, X. Description: Crystal structure of the G26C/Q250A mutant of LeuT [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 7djc is ON HOLD

Authors: Fan, J., Xiao, Y., Sun, Z., Zhou, X.

Description: Crystal structure of the G26C/Q250A mutant of LeuT
[[Category: Unreleased Structures]]
[[Category: Xiao, Y]]
[[Category: Fan, J]]
[[Category: Zhou, X]]
[[Category: Sun, Z]]