OCA at 16:38, 29 November 2023

OCA — Wed, 29 Nov 2023 16:38:28 GMT

←Older revision		Revision as of 16:38, 29 November 2023
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	==Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212121==		==Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212121==
-	<StructureSection load='7dr9' size='340' side='right'caption='[[7dr9]]' scene=''>	+	<StructureSection load='7dr9' size='340' side='right'caption='[[7dr9]], [[Resolution\|resolution]] 2.78Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DR9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dr9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Middle_East_respiratory_syndrome-related_coronavirus Middle East respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DR9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dr9 OCA], [https://pdbe.org/7dr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dr9 RCSB], [https://www.ebi.ac.uk/pdbsum/7dr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dr9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.775871Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dr9 OCA], [https://pdbe.org/7dr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dr9 RCSB], [https://www.ebi.ac.uk/pdbsum/7dr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dr9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/R1AB_MERS1 R1AB_MERS1] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.[UniProtKB:P0C6X7] Promotes the degradation of host mRNAs by inducing an endonucleolytic RNA cleavage in template mRNAs, and inhibits of host mRNA translation, a function that is separable from its RNA cleavage activity. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.<ref>PMID:26311885</ref> May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[UniProtKB:P0C6X7] Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates, together with nsp4, in the assembly of virally induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B. signaling.[UniProtKB:P0C6X7]<ref>PMID:25142582</ref> Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[UniProtKB:P0C6X7] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[UniProtKB:P0C6X7][PROSITE-ProRule:PRU00772] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[UniProtKB:P0C6X7] May participate in viral replication by acting as a ssRNA-binding protein.[UniProtKB:P0C6X7] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[UniProtKB:P0C6X7] Responsible for replication and transcription of the viral RNA genome.[UniProtKB:P0C6X7] Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.[UniProtKB:P0C6X7] Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.[UniProtKB:P0C6X7] Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR (By similarity). Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (By similarity). If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors (By similarity).[UniProtKB:P0C6X7] Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.[UniProtKB:P0C6X7]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Middle East respiratory syndrome-related coronavirus]]
	[[Category: Li J]]		[[Category: Li J]]
	[[Category: Lin C]]		[[Category: Lin C]]
	[[Category: Zhang J]]		[[Category: Zhang J]]
	[[Category: Zhong FL]]		[[Category: Zhong FL]]

OCA at 14:07, 29 December 2021

OCA — Wed, 29 Dec 2021 14:07:47 GMT

←Older revision		Revision as of 14:07, 29 December 2021
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-	'''Unreleased structure'''

-	~~The entry 7dr9 is ON HOLD until Dec 27 2022~~	+	==Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212121==
-		+	<StructureSection load='7dr9' size='340' side='right'caption='[[7dr9]]' scene=''>
-	~~Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DR9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DR9 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212121	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dr9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dr9 OCA], [https://pdbe.org/7dr9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dr9 RCSB], [https://www.ebi.ac.uk/pdbsum/7dr9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dr9 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Lin~~, C]]	+	__TOC__
-	[[Category: Li, J]]	+	</StructureSection>
-	[[Category: ~~Zhong, F.L~~]]	+	[[Category: Large Structures]]
-	[[Category: Zhang, J]]	+	[[Category: Li J]]
		+	[[Category: Lin C]]
		+	[[Category: Zhang J]]
		+	[[Category: Zhong FL]]

OCA at 07:44, 21 July 2021

OCA — Wed, 21 Jul 2021 07:44:40 GMT

←Older revision		Revision as of 07:44, 21 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7dr9 is ON HOLD	+	The entry 7dr9 is ON HOLD until Dec 27 2022

	Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J.		Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J.

OCA: Protected "7dr9" [edit=sysop:move=sysop]

OCA — Wed, 06 Jan 2021 05:37:24 GMT

Protected "7dr9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 6 January 2021

OCA: New page: '''Unreleased structure''' The entry 7dr9 is ON HOLD Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J. Description: Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212...

OCA — Wed, 06 Jan 2021 05:37:21 GMT

New page: '''Unreleased structure''' The entry 7dr9 is ON HOLD Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J. Description: Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212...

New page

'''Unreleased structure'''

The entry 7dr9 is ON HOLD

Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J.

Description: Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212121
[[Category: Unreleased Structures]]
[[Category: Lin, C]]
[[Category: Li, J]]
[[Category: Zhong, F.L]]
[[Category: Zhang, J]]

7dr9 - Revision history

OCA at 16:38, 29 November 2023

OCA at 14:07, 29 December 2021

OCA at 07:44, 21 July 2021

OCA: Protected "7dr9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7dr9 is ON HOLD Authors: Zhong, F.L., Lin, C., Zhang, J., Li, J. Description: Crystal structure of MERS-CoV 3CL protease (C148A) in spacegroup P212...