7dvl - Revision history

OCA at 16:40, 29 November 2023

OCA — Wed, 29 Nov 2023 16:40:24 GMT

←Older revision		Revision as of 16:40, 29 November 2023
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	<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7dvl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dvl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.006Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>bont, FDC09_07905 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1491~~ "~~Bacillus botulinus~~" ~~van Ermengem 1896])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D3IV24_CLOBO D3IV24_CLOBO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus botulinus van ermengem 1896~~]]	+	[[Category: Clostridium botulinum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kammerer, R]]	+	[[Category: Kammerer R]]
-	[[Category: Leka, O]]	+	[[Category: Leka O]]
-	[[Category: Li, X]]	+	[[Category: Li X]]
-	[[Category: Wu, Y]]	+	[[Category: Wu Y]]
-	~~[[Category: Activity]]~~	+
-	~~[[Category: Botulinum neurotoxin]]~~	+
-	~~[[Category: Catalytic domain]]~~	+
-	~~[[Category: Stability]]~~	+
-	~~[[Category: Toxin~~]]	+

OCA at 10:06, 14 July 2021

OCA — Wed, 14 Jul 2021 10:06:26 GMT

←Older revision		Revision as of 10:06, 14 July 2021
Line 3:		Line 3:
	<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]], [[Resolution\|resolution]] 2.01Å' scene=''>		<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7dvl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dvl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_botulinus"_van_ermengem_1896 "bacillus botulinus" van ermengem 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bont, FDC09_07905 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1491 "Bacillus botulinus" van Ermengem 1896])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus botulinus van ermengem 1896]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kammerer, R]]		[[Category: Kammerer, R]]

OCA at 09:12, 5 May 2021

OCA — Wed, 05 May 2021 09:12:05 GMT

←Older revision		Revision as of 09:12, 5 May 2021
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	==Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3==		==Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3==
-	<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]]' scene=''>	+	<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7dvl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Botulinum neurotoxins (BoNTs) are among the most widely used therapeutic proteins; however, only two subtypes within the seven serotypes, BoNT/A1 and BoNT/B1, are currently used for medical and cosmetic applications. Distinct catalytic properties, substrate specificities, and duration of enzymatic activities potentially make other subtypes very attractive candidates to outperform conventional BoNTs in particular therapeutic applications. For example, BoNT/A3 has a significantly shorter duration of action than other BoNT/A subtypes. Notably, BoNT/A3 is the subtype with the least conserved catalytic domain among BoNT/A subtypes. This suggests that the sequence differences, many of which concern the alpha-exosite, contribute to the observed functional differences in toxin persistence by affecting the binding of the substrate SNAP-25 and/or the stability of the catalytic domain fold. To identify the molecular determinants accounting for the differences in the persistence observed for BoNT/A subtypes, we determined the crystal structure of the catalytic domain of BoNT/A3 (LC/A3). The structure of LC/A3 was found to be very similar to that of LC/A1, suggesting that the overall mode of SNAP-25 binding is common between these two proteins. However, circular dichroism (CD) thermal unfolding experiments demonstrated that LC/A3 is significantly less stable than LC/A1, implying that this might contribute to the reduced toxin persistence of BoNT/A3. These findings could be of interest in developing next generation therapeutic toxins.
		+
		+	Crystal structure of the catalytic domain of botulinum neurotoxin subtype A3.,Leka O, Wu Y, Li X, Kammerer RA J Biol Chem. 2021 Apr 20:100684. doi: 10.1016/j.jbc.2021.100684. PMID:33891946<ref>PMID:33891946</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7dvl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kammerer R]]	+	[[Category: Kammerer, R]]
-	[[Category: Leka O]]	+	[[Category: Leka, O]]
-	[[Category: Li X]]	+	[[Category: Li, X]]
-	[[Category: Wu Y]]	+	[[Category: Wu, Y]]
		+	[[Category: Activity]]
		+	[[Category: Botulinum neurotoxin]]
		+	[[Category: Catalytic domain]]
		+	[[Category: Stability]]
		+	[[Category: Toxin]]

OCA at 05:21, 28 April 2021

OCA — Wed, 28 Apr 2021 05:21:09 GMT

←Older revision		Revision as of 05:21, 28 April 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7dvl ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3==
-		+	<StructureSection load='7dvl' size='340' side='right'caption='[[7dvl]]' scene=''>
-	~~Authors~~: Wu, Y., ~~Leka~~, O., ~~Kammerer, R~~., ~~Li, X~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVL FirstGlance]. <br>
-	~~Description: Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvl OCA], [https://pdbe.org/7dvl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvl RCSB], [https://www.ebi.ac.uk/pdbsum/7dvl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Leka, O~~]]	+	__TOC__
-	[[Category: ~~Li, X~~]]	+	</StructureSection>
-	[[Category: ~~Wu, Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Kammerer, R~~]]	+	[[Category: Kammerer R]]
		+	[[Category: Leka O]]
		+	[[Category: Li X]]
		+	[[Category: Wu Y]]

OCA at 05:31, 24 February 2021

OCA — Wed, 24 Feb 2021 05:31:07 GMT

←Older revision		Revision as of 05:31, 24 February 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7dvl is ON HOLD	+	The entry 7dvl is ON HOLD until Paper Publication

	Authors: Wu, Y., Leka, O., Kammerer, R., Li, X.		Authors: Wu, Y., Leka, O., Kammerer, R., Li, X.
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	Description: Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3		Description: Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wu, Y]]
-	[[Category: Kammerer, R]]
	[[Category: Leka, O]]		[[Category: Leka, O]]
	[[Category: Li, X]]		[[Category: Li, X]]
		+	[[Category: Wu, Y]]
		+	[[Category: Kammerer, R]]

OCA: Protected "7dvl" [edit=sysop:move=sysop]

OCA — Wed, 20 Jan 2021 06:49:39 GMT

Protected "7dvl" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:49, 20 January 2021

OCA: New page: '''Unreleased structure''' The entry 7dvl is ON HOLD Authors: Wu, Y., Leka, O., Kammerer, R., Li, X. Description: Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subt...

OCA — Wed, 20 Jan 2021 06:49:38 GMT

New page: '''Unreleased structure''' The entry 7dvl is ON HOLD Authors: Wu, Y., Leka, O., Kammerer, R., Li, X. Description: Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subt...

New page

'''Unreleased structure'''

The entry 7dvl is ON HOLD

Authors: Wu, Y., Leka, O., Kammerer, R., Li, X.

Description: Crystal Structure of the Catalytic Domain of Botulinum Neurotoxin Subtype A3
[[Category: Unreleased Structures]]
[[Category: Wu, Y]]
[[Category: Kammerer, R]]
[[Category: Leka, O]]
[[Category: Li, X]]