OCA at 16:46, 29 November 2023

OCA — Wed, 29 Nov 2023 16:46:00 GMT

←Older revision		Revision as of 16:46, 29 November 2023
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	==Structural insights into a bifunctional peptide methionine sulfoxide reductase MsrA/B fusion protein from Helicobacter pylori==		==Structural insights into a bifunctional peptide methionine sulfoxide reductase MsrA/B fusion protein from Helicobacter pylori==
-	<StructureSection load='7e43' size='340' side='right'caption='[[7e43]]' scene=''>	+	<StructureSection load='7e43' size='340' side='right'caption='[[7e43]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E43 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7e43]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E43 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e43 OCA], [https://pdbe.org/7e43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e43 RCSB], [https://www.ebi.ac.uk/pdbsum/7e43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e43 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e43 OCA], [https://pdbe.org/7e43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e43 RCSB], [https://www.ebi.ac.uk/pdbsum/7e43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e43 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MSRAB_HELPY MSRAB_HELPY] Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methionine sulfoxide reductase (Msr) is a family of enzymes that reduces oxidized methionine and plays an important role in the survival of bacteria under oxidative stress conditions. MsrA and MsrB exist in a fusion protein form (MsrAB) in some pathogenic bacteria, such as Helicobacter pylori (Hp), Streptococcus pneumoniae, and Treponema denticola. To understand the fused form instead of the separated enzyme at the molecular level, we determined the crystal structure of HpMsrAB(C44S/C318S) at 2.2 A, which showed that a linker region (Hpiloop, 193-205) between two domains interacted with each HpMsrA or HpMsrB domain via three salt bridges (E193-K107, D197-R103, and K200-D339). Two acetate molecules in the active site pocket showed an sp(2) planar electron density map in the crystal structure, which interacted with the conserved residues in fusion MsrABs from the pathogen. Biochemical and kinetic analyses revealed that Hpiloop is required to increase the catalytic efficiency of HpMsrAB. Two salt bridge mutants (D193A and E199A) were located at the entrance or tailgate of Hpiloop. Therefore, the linker region of the MsrAB fusion enzyme plays a key role in the structural stability and catalytic efficiency and provides a better understanding of why MsrAB exists in a fused form.
		+
		+	Structural Insights into a Bifunctional Peptide Methionine Sulfoxide Reductase MsrA/B Fusion Protein from Helicobacter pylori.,Kim S, Lee K, Park SH, Kwak GH, Kim MS, Kim HY, Hwang KY Antioxidants (Basel). 2021 Mar 5;10(3). pii: antiox10030389. doi:, 10.3390/antiox10030389. PMID:33807684<ref>PMID:33807684</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7e43" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Helicobacter pylori 26695]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hwang KY]]		[[Category: Hwang KY]]
	[[Category: Kim S]]		[[Category: Kim S]]
	[[Category: Lee K]]		[[Category: Lee K]]

OCA at 08:23, 21 April 2021

OCA — Wed, 21 Apr 2021 08:23:32 GMT

←Older revision		Revision as of 08:23, 21 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7e43 is ~~ON HOLD until Paper Publication~~	+	==Structural insights into a bifunctional peptide methionine sulfoxide reductase MsrA/B fusion protein from Helicobacter pylori==
-		+	<StructureSection load='7e43' size='340' side='right'caption='[[7e43]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E43 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e43 OCA], [https://pdbe.org/7e43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e43 RCSB], [https://www.ebi.ac.uk/pdbsum/7e43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e43 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hwang KY]]
		+	[[Category: Kim S]]
		+	[[Category: Lee K]]

OCA at 09:13, 31 March 2021

OCA — Wed, 31 Mar 2021 09:13:53 GMT

←Older revision		Revision as of 09:13, 31 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7e43 is ON HOLD	+	The entry 7e43 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7e43" [edit=sysop:move=sysop]

OCA — Wed, 17 Feb 2021 08:39:33 GMT

Protected "7e43" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:39, 17 February 2021

OCA: New page: '''Unreleased structure''' The entry 7e43 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Feb 2021 08:39:32 GMT

New page: '''Unreleased structure''' The entry 7e43 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7e43 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7e43 - Revision history

OCA at 16:46, 29 November 2023

OCA at 08:23, 21 April 2021

OCA at 09:13, 31 March 2021

OCA: Protected "7e43" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7e43 is ON HOLD Authors: Description: Category: Unreleased Structures