7e6m - Revision history

OCA at 16:47, 29 November 2023

OCA — Wed, 29 Nov 2023 16:47:33 GMT

←Older revision		Revision as of 16:47, 29 November 2023
Line 3:		Line 3:
	<StructureSection load='7e6m' size='340' side='right'caption='[[7e6m]], [[Resolution\|resolution]] 1.83Å' scene=''>		<StructureSection load='7e6m' size='340' side='right'caption='[[7e6m]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7e6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E6M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7e6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_coronavirus_NL63 Human coronavirus NL63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E6M FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e6m OCA], [https://pdbe.org/7e6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e6m RCSB], [https://www.ebi.ac.uk/pdbsum/7e6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e6m ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8344502Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e6m OCA], [https://pdbe.org/7e6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e6m RCSB], [https://www.ebi.ac.uk/pdbsum/7e6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e6m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/R1A_CVHNL R1A_CVHNL]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3. The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).	+	[https://www.uniprot.org/uniprot/R1A_CVHNL R1A_CVHNL] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3. The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human coronavirus NL63]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao~~, H X~~]]	+	[[Category: Gao HX]]
-	[[Category: Li, J]]	+	[[Category: Li J]]
-	[[Category: Zhang, J]]	+	[[Category: Zhang J]]
-	[[Category: Zhang~~, Y T~~]]	+	[[Category: Zhang YT]]
-	[[Category: Zhong~~, F L~~]]	+	[[Category: Zhong FL]]
-	[[Category: Zhou~~, X L]]~~	+	[[Category: Zhou XL]]
-	~~[[Category: Hcov-nl63 3c-like protease]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 12:51, 13 October 2021

OCA — Wed, 13 Oct 2021 12:51:58 GMT

←Older revision		Revision as of 12:51, 13 October 2021
Line 1:		Line 1:

	==Crystal structure of Human coronavirus NL63 3C-like protease==		==Crystal structure of Human coronavirus NL63 3C-like protease==
-	<StructureSection load='7e6m' size='340' side='right'caption='[[7e6m]]' scene=''>	+	<StructureSection load='7e6m' size='340' side='right'caption='[[7e6m]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E6M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7e6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E6M FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e6m OCA], [https://pdbe.org/7e6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e6m RCSB], [https://www.ebi.ac.uk/pdbsum/7e6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e6m ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e6m OCA], [https://pdbe.org/7e6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e6m RCSB], [https://www.ebi.ac.uk/pdbsum/7e6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e6m ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/R1A_CVHNL R1A_CVHNL]] The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3. The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function (By similarity). Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity). Nsp9 is a ssRNA-binding protein (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human coronavirus NL63 (HCoV-NL63), which belongs to the genus Alphacoronavirus, mainly infects children and the immunocompromized and is responsible for a series of clinical manifestations, including cough, fever, rhinorrhoea, bronchiolitis and croup. HCoV-NL63, which was first isolated from a seven-month-old child in 2004, has led to infections worldwide and accounts for 10% of all respiratory illnesses caused by etiological agents. However, effective antivirals against HCoV-NL63 infection are currently unavailable. The HCoV-NL63 main protease (M(pro)), also called 3C-like protease (3CL(pro)), plays a vital role in mediating viral replication and transcription by catalyzing the cleavage of replicase polyproteins (pp1a and pp1ab) into functional subunits. Moreover, M(pro) is highly conserved among all coronaviruses, thus making it a prominent drug target for antiviral therapy. Here, four crystal structures of HCoV-NL63 M(pro) in the apo form at different pH values are reported at resolutions of up to 1.78 A. Comparison with M(pro) from other human betacoronaviruses such as SARS-CoV-2 and SARS-CoV reveals common and distinct structural features in different genera and extends knowledge of the diversity, function and evolution of coronaviruses.
		+
		+	Crystal structures of human coronavirus NL63 main protease at different pH values.,Gao H, Zhang Y, Jiang H, Hu X, Zhang Y, Zhou X, Zhong F, Lin C, Li J, Luo J, Zhang J Acta Crystallogr F Struct Biol Commun. 2021 Oct 1;77(Pt 10):348-355. doi:, 10.1107/S2053230X21009523. Epub 2021 Sep 27. PMID:34605439<ref>PMID:34605439</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7e6m" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao HX]]	+	[[Category: Gao, H X]]
-	[[Category: Li J]]	+	[[Category: Li, J]]
-	[[Category: Zhang J]]	+	[[Category: Zhang, J]]
-	[[Category: Zhang YT]]	+	[[Category: Zhang, Y T]]
-	[[Category: Zhong FL]]	+	[[Category: Zhong, F L]]
-	[[Category: Zhou XL]]	+	[[Category: Zhou, X L]]
		+	[[Category: Hcov-nl63 3c-like protease]]
		+	[[Category: Viral protein]]

OCA at 05:30, 6 October 2021

OCA — Wed, 06 Oct 2021 05:30:08 GMT

←Older revision		Revision as of 05:30, 6 October 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7e6m ~~is ON HOLD~~	+	==Crystal structure of Human coronavirus NL63 3C-like protease==
-		+	<StructureSection load='7e6m' size='340' side='right'caption='[[7e6m]]' scene=''>
-	~~Authors~~: ~~Gao, H~~.X.~~, Zhang, Y~~.T., ~~Zhong, F~~.L., ~~Zhou~~, X.L., ~~Li, J~~., ~~Zhang, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E6M FirstGlance]. <br>
-	~~Description: Crystal structure of Human coronavirus NL63 3C-like protease~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e6m OCA], [https://pdbe.org/7e6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e6m RCSB], [https://www.ebi.ac.uk/pdbsum/7e6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e6m ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Gao~~, H.X~~]]	+	__TOC__
-	[[Category: ~~Zhang, Y.T~~]]	+	</StructureSection>
-	[[Category: ~~Li,~~ J]]	+	[[Category: Large Structures]]
-	[[Category: ~~Zhong, F.L~~]]	+	[[Category: Gao HX]]
-	[[Category: ~~Zhang, J~~]]	+	[[Category: Li J]]
-	[[Category: Zhou~~, X.L~~]]	+	[[Category: Zhang J]]
		+	[[Category: Zhang YT]]
		+	[[Category: Zhong FL]]
		+	[[Category: Zhou XL]]

OCA: Protected "7e6m" [edit=sysop:move=sysop]

OCA — Wed, 03 Mar 2021 13:18:16 GMT

Protected "7e6m" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:18, 3 March 2021

OCA: New page: '''Unreleased structure''' The entry 7e6m is ON HOLD Authors: Gao, H.X., Zhang, Y.T., Zhong, F.L., Zhou, X.L., Li, J., Zhang, J. Description: Crystal structure of Human coronavirus NL6...

OCA — Wed, 03 Mar 2021 13:18:14 GMT

New page: '''Unreleased structure''' The entry 7e6m is ON HOLD Authors: Gao, H.X., Zhang, Y.T., Zhong, F.L., Zhou, X.L., Li, J., Zhang, J. Description: Crystal structure of Human coronavirus NL6...

New page

'''Unreleased structure'''

The entry 7e6m is ON HOLD

Authors: Gao, H.X., Zhang, Y.T., Zhong, F.L., Zhou, X.L., Li, J., Zhang, J.

Description: Crystal structure of Human coronavirus NL63 3C-like protease
[[Category: Unreleased Structures]]
[[Category: Gao, H.X]]
[[Category: Zhang, Y.T]]
[[Category: Li, J]]
[[Category: Zhong, F.L]]
[[Category: Zhang, J]]
[[Category: Zhou, X.L]]