OCA at 16:50, 29 November 2023

OCA — Wed, 29 Nov 2023 16:50:26 GMT

←Older revision		Revision as of 16:50, 29 November 2023
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-	====	+	==Crystal structure of SdgB (complexed with peptides)==
-	<StructureSection load='7ec6' size='340' side='right'caption='[[7ec6]]' scene=''>	+	<StructureSection load='7ec6' size='340' side='right'caption='[[7ec6]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ec6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_USA300 Staphylococcus aureus subsp. aureus USA300] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EC6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ec6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ec6 OCA], [https://pdbe.org/7ec6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ec6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ec6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ec6 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ec6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ec6 OCA], [https://pdbe.org/7ec6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ec6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ec6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ec6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q2G0L2_STAA8 Q2G0L2_STAA8]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The initiation of infection of host tissues by Staphylococcus aureus requires a family of staphylococcal adhesive proteins containing serine-aspartate repeat (SDR) domains, such as ClfA. The O-linked glycosylation of the long-chain SDR domain mediated by SdgB and SdgA is a key virulence factor that protects the adhesive SDR proteins against host proteolytic attack in order to promote successful tissue colonization, and has also been implicated in staphylococcal agglutination, which leads to sepsis and an immunodominant epitope for a strong antibody response. Despite the biological significance of these two glycosyltransferases involved in pathogenicity and avoidance of the host innate immune response, their structures and the molecular basis of their activity have not been investigated. This study reports the crystal structures of SdgB and SdgA from S. aureus as well as multiple structures of SdgB in complex with its substrates (for example UDP, N-acetylglucosamine or SDR peptides), products (glycosylated SDR peptides) or phosphate ions. Together with biophysical and biochemical analyses, this structural work uncovered the novel mechanism by which SdgB and SdgA carry out the glycosyl-transfer process to the long SDR region in SDR proteins. SdgB undergoes dynamic changes in its structure such as a transition from an open to a closed conformation upon ligand binding and takes diverse forms, both as a homodimer and as a heterodimer with SdgA. Overall, these findings not only elucidate the putative role of the three domains of SdgB in recognizing donor and acceptor substrates, but also provide new mechanistic insights into glycosylation of the SDR domain, which can serve as a starting point for the development of antibacterial drugs against staphylococcal infections.
		+
		+	Structural basis for SdgB- and SdgA-mediated glycosylation of staphylococcal adhesive proteins.,Kim DG, Baek I, Lee Y, Kim H, Kim JY, Bang G, Kim S, Yoon HJ, Han BW, Suh SW, Kim HS Acta Crystallogr D Struct Biol. 2021 Nov 1;77(Pt 11):1460-1474. doi:, 10.1107/S2059798321010068. Epub 2021 Oct 20. PMID:34726173<ref>PMID:34726173</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ec6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Staphylococcus aureus subsp. aureus USA300]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Baek I]]
		+	[[Category: Kim D-G]]
		+	[[Category: Kim HS]]
		+	[[Category: Lee Y]]

OCA at 06:12, 16 March 2022

OCA — Wed, 16 Mar 2022 06:12:26 GMT

←Older revision		Revision as of 06:12, 16 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ec6 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ec6' size='340' side='right'caption='[[7ec6]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ec6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ec6 OCA], [https://pdbe.org/7ec6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ec6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ec6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ec6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:22, 24 March 2021

OCA — Wed, 24 Mar 2021 05:22:19 GMT

←Older revision		Revision as of 05:22, 24 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ec6 is ON HOLD	+	The entry 7ec6 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ec6" [edit=sysop:move=sysop]

OCA — Wed, 17 Mar 2021 05:20:30 GMT

Protected "7ec6" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 17 March 2021

OCA: New page: '''Unreleased structure''' The entry 7ec6 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Mar 2021 05:20:29 GMT

New page: '''Unreleased structure''' The entry 7ec6 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ec6 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7ec6 - Revision history

OCA at 16:50, 29 November 2023

OCA at 06:12, 16 March 2022

OCA at 05:22, 24 March 2021

OCA: Protected "7ec6" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ec6 is ON HOLD Authors: Description: Category: Unreleased Structures