7egv - Revision history

OCA at 16:53, 29 November 2023

OCA — Wed, 29 Nov 2023 16:53:29 GMT

←Older revision		Revision as of 16:53, 29 November 2023
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	==Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid==		==Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid==
-	<StructureSection load='7egv' size='340' side='right'caption='[[7egv]]' scene=''>	+	<StructureSection load='7egv' size='340' side='right'caption='[[7egv]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EGV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7egv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichoderma_harzianum Trichoderma harzianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EGV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7egv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7egv OCA], [https://pdbe.org/7egv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7egv RCSB], [https://www.ebi.ac.uk/pdbsum/7egv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7egv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.54Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=J3L:(2S)-3-methyl-2-[[(2S,4R)-1-methyl-4-[(2E,4E)-octa-2,4-dienoyl]-3,5-bis(oxidanylidene)pyrrolidin-2-yl]methyl]-2-oxidanyl-butanoic+acid'>J3L</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TZD:2-{3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-4-METHYL-2-OXO-2,3-DIHYDRO-1,3-THIAZOL-5-YL}ETHYL+TRIHYDROGEN+DIPHOSPHATE'>TZD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7egv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7egv OCA], [https://pdbe.org/7egv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7egv RCSB], [https://www.ebi.ac.uk/pdbsum/7egv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7egv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A2N1LPC4_TRIHA A0A2N1LPC4_TRIHA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acetohydroxyacid synthase (AHAS) is the first enzyme in the branched-chain amino acid biosynthetic pathway and is a validated target for herbicide and fungicide development. Here we report harzianic acid (HA, 1) produced by the biocontrol fungus Trichoderma afroharzianum t-22 (Tht22) as a natural product inhibitor of AHAS. The biosynthetic pathway of HA was elucidated with heterologous reconstitution. Guided by a putative self-resistance enzyme in the genome, HA was biochemically demonstrated to be a selective inhibitor of fungal AHAS, including those from phytopathogenic fungi. In addition, HA can inhibit a common resistant variant of AHAS in which the active site proline is mutated. Structural analysis of AHAS complexed with HA revealed the molecular basis of competitive inhibition, which differs from all known commercial AHAS inhibitors. The alternative binding mode also rationalizes the selectivity of HA, as well as effectiveness toward resistant mutants. A proposed role of HA biosynthesis by Tht22 in the rhizosphere is discussed based on the data.
		+
		+	Harzianic Acid from Trichoderma afroharzianum Is a Natural Product Inhibitor of Acetohydroxyacid Synthase.,Xie L, Zang X, Cheng W, Zhang Z, Zhou J, Chen M, Tang Y J Am Chem Soc. 2021 Jun 16. doi: 10.1021/jacs.1c03988. PMID:34132537<ref>PMID:34132537</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7egv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Trichoderma harzianum]]
	[[Category: Chen M]]		[[Category: Chen M]]
	[[Category: Tang Y]]		[[Category: Tang Y]]

OCA at 03:33, 2 July 2021

OCA — Fri, 02 Jul 2021 03:33:23 GMT

←Older revision		Revision as of 03:33, 2 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7egv ~~is ON HOLD until Paper Publication~~	+	==Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid==
-		+	<StructureSection load='7egv' size='340' side='right'caption='[[7egv]]' scene=''>
-	~~Authors~~: ~~Zang~~, X., ~~Xie, L~~., ~~Chen, M~~., ~~Tang, Y~~., ~~Zhou, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EGV FirstGlance]. <br>
-	~~Description: Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7egv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7egv OCA], [https://pdbe.org/7egv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7egv RCSB], [https://www.ebi.ac.uk/pdbsum/7egv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7egv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Chen, M]]	+	__TOC__
-	[[Category: Tang, Y]]	+	</StructureSection>
-	[[Category: ~~Zang, X~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Zhou, J~~]]	+	[[Category: Chen M]]
-	[[Category: ~~Xie, L~~]]	+	[[Category: Tang Y]]
		+	[[Category: Xie L]]
		+	[[Category: Zang X]]
		+	[[Category: Zhou J]]

OCA at 05:34, 14 April 2021

OCA — Wed, 14 Apr 2021 05:34:18 GMT

←Older revision		Revision as of 05:34, 14 April 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7egv is ON HOLD	+	The entry 7egv is ON HOLD until Paper Publication

-	Authors: Zang, X., ~~Zhou~~, J., Tang, Y.	+	Authors: Zang, X., Xie, L., Chen, M., Tang, Y., Zhou, J.

	Description: Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid		Description: Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Chen, M]]
	[[Category: Tang, Y]]		[[Category: Tang, Y]]
	[[Category: Zang, X]]		[[Category: Zang, X]]
	[[Category: Zhou, J]]		[[Category: Zhou, J]]
		+	[[Category: Xie, L]]

OCA: Protected "7egv" [edit=sysop:move=sysop]

OCA — Wed, 31 Mar 2021 09:19:18 GMT

Protected "7egv" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:19, 31 March 2021

OCA: New page: '''Unreleased structure''' The entry 7egv is ON HOLD Authors: Zang, X., Zhou, J., Tang, Y. Description: Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid [...

OCA — Wed, 31 Mar 2021 09:19:17 GMT

New page: '''Unreleased structure''' The entry 7egv is ON HOLD Authors: Zang, X., Zhou, J., Tang, Y. Description: Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid [...

New page

'''Unreleased structure'''

The entry 7egv is ON HOLD

Authors: Zang, X., Zhou, J., Tang, Y.

Description: Acetolactate Synthase from Trichoderma harzianum with inhibitor harzianic acid
[[Category: Unreleased Structures]]
[[Category: Tang, Y]]
[[Category: Zang, X]]
[[Category: Zhou, J]]