7eio - Revision history

OCA at 19:33, 29 May 2024

OCA — Wed, 29 May 2024 19:33:35 GMT

←Older revision		Revision as of 19:33, 29 May 2024
Line 3:		Line 3:
	<StructureSection load='7eio' size='340' side='right'caption='[[7eio]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='7eio' size='340' side='right'caption='[[7eio]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7eio]] is a 2 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EIO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.895Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eio OCA], [https://pdbe.org/7eio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eio RCSB], [https://www.ebi.ac.uk/pdbsum/7eio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eio ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eio OCA], [https://pdbe.org/7eio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eio RCSB], [https://www.ebi.ac.uk/pdbsum/7eio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eio ProSAT]</span></td></tr>
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/MEI2_SCHPO MEI2_SCHPO]] Crucial for commitment to meiosis but it is not sufficient itself for the commitment. May be a splicing regulator.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In the fission yeast Schizosaccharomyces pombe, Mei2, an RNA-binding protein essential for entry into meiosis, regulates meiosis initiation. Mei2 binds to a specific non-coding RNA species, meiRNA, and accumulates at sme2 gene locus, which encodes meiRNA. Previous research has shown that the Mei2 C-terminal RNA recognition motif (RRM3) physically interacts with meiRNA 5' region in vitro and stimulates meiosis in vivo. However, the underlying mechanism still remains elusive. We first employed an in vitro crosslinking and immunoprecipitation sequencing (CLIP-seq) assay and demonstrated a preference for U-rich motifs of meiRNA by Mei2 RRM3. We then solved the crystal structures of Mei2 RRM3 in the apo form and complex with an 8mer RNA fragment, derived from meiRNA, as detected by in vitro CLIP-seq. These results provide structural insights into Mei2 RRM3-meiRNA complex and reveal that Mei2 RRM3 binds specifically to the UUC(U) sequence. Furthermore, a structure-based Mei2 mutation, Mei2F644A causes defective karyogamy, suggesting an essential role of the RNA-binding ability of Mei2 in regulating meiosis.
-
-	Structural insights reveal the specific recognition of meiRNA by the Mei2 protein.,Shen S, Jian Y, Cai Z, Li F, Lv M, Liu Y, Wu J, Fu C, Shi Y J Mol Cell Biol. 2022 May 5. pii: 6581319. doi: 10.1093/jmcb/mjac029. PMID:35512546<ref>PMID:35512546</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7eio" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li~~, F D~~]]	+	[[Category: Li FD]]
-	[[Category: Shen~~, S Y]]~~	+	[[Category: Shen SY]]
-	~~[[Category: Mei2]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rrm~~]]	+

OCA at 10:20, 18 May 2022

OCA — Wed, 18 May 2022 10:20:47 GMT

←Older revision		Revision as of 10:20, 18 May 2022
Line 1:		Line 1:

	==Crystal Structure of Mei2 RRM3==		==Crystal Structure of Mei2 RRM3==
-	<StructureSection load='7eio' size='340' side='right'caption='[[7eio]]' scene=''>	+	<StructureSection load='7eio' size='340' side='right'caption='[[7eio]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EIO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eio]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EIO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eio OCA], [https://pdbe.org/7eio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eio RCSB], [https://www.ebi.ac.uk/pdbsum/7eio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eio ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eio OCA], [https://pdbe.org/7eio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eio RCSB], [https://www.ebi.ac.uk/pdbsum/7eio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eio ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/MEI2_SCHPO MEI2_SCHPO]] Crucial for commitment to meiosis but it is not sufficient itself for the commitment. May be a splicing regulator.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the fission yeast Schizosaccharomyces pombe, Mei2, an RNA-binding protein essential for entry into meiosis, regulates meiosis initiation. Mei2 binds to a specific non-coding RNA species, meiRNA, and accumulates at sme2 gene locus, which encodes meiRNA. Previous research has shown that the Mei2 C-terminal RNA recognition motif (RRM3) physically interacts with meiRNA 5' region in vitro and stimulates meiosis in vivo. However, the underlying mechanism still remains elusive. We first employed an in vitro crosslinking and immunoprecipitation sequencing (CLIP-seq) assay and demonstrated a preference for U-rich motifs of meiRNA by Mei2 RRM3. We then solved the crystal structures of Mei2 RRM3 in the apo form and complex with an 8mer RNA fragment, derived from meiRNA, as detected by in vitro CLIP-seq. These results provide structural insights into Mei2 RRM3-meiRNA complex and reveal that Mei2 RRM3 binds specifically to the UUC(U) sequence. Furthermore, a structure-based Mei2 mutation, Mei2F644A causes defective karyogamy, suggesting an essential role of the RNA-binding ability of Mei2 in regulating meiosis.
		+
		+	Structural insights reveal the specific recognition of meiRNA by the Mei2 protein.,Shen S, Jian Y, Cai Z, Li F, Lv M, Liu Y, Wu J, Fu C, Shi Y J Mol Cell Biol. 2022 May 5. pii: 6581319. doi: 10.1093/jmcb/mjac029. PMID:35512546<ref>PMID:35512546</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eio" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li FD]]	+	[[Category: Li, F D]]
-	[[Category: Shen SY]]	+	[[Category: Shen, S Y]]
		+	[[Category: Mei2]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rrm]]

OCA at 10:22, 6 April 2022

OCA — Wed, 06 Apr 2022 10:22:30 GMT

←Older revision		Revision as of 10:22, 6 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7eio ~~is ON HOLD until Jun 30 2023~~	+	==Crystal Structure of Mei2 RRM3==
-		+	<StructureSection load='7eio' size='340' side='right'caption='[[7eio]]' scene=''>
-	~~Authors~~: ~~Shen~~, S.Y., Li, F.D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EIO FirstGlance]. <br>
-	~~Description: Crystal Structure of Mei2 RRM3~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eio OCA], [https://pdbe.org/7eio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eio RCSB], [https://www.ebi.ac.uk/pdbsum/7eio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eio ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Li~~, F.D~~]]	+	__TOC__
-	[[Category: Shen~~, S.Y~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Li FD]]
		+	[[Category: Shen SY]]

OCA at 04:45, 9 March 2022

OCA — Wed, 09 Mar 2022 04:45:08 GMT

←Older revision		Revision as of 04:45, 9 March 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eio is ON HOLD until ~~Mar 31~~ 2023	+	The entry 7eio is ON HOLD until Jun 30 2023

	Authors: Shen, S.Y., Li, F.D.		Authors: Shen, S.Y., Li, F.D.
Line 7:		Line 7:
	Description: Crystal Structure of Mei2 RRM3		Description: Crystal Structure of Mei2 RRM3
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Shen, S.Y]]
	[[Category: Li, F.D]]		[[Category: Li, F.D]]
		+	[[Category: Shen, S.Y]]

OCA at 06:02, 21 April 2021

OCA — Wed, 21 Apr 2021 06:02:03 GMT

←Older revision		Revision as of 06:02, 21 April 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eio is ON HOLD	+	The entry 7eio is ON HOLD until Mar 31 2023

	Authors: Shen, S.Y., Li, F.D.		Authors: Shen, S.Y., Li, F.D.

OCA: Protected "7eio" [edit=sysop:move=sysop]

OCA — Wed, 07 Apr 2021 04:32:40 GMT

Protected "7eio" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:32, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7eio is ON HOLD Authors: Shen, S.Y., Li, F.D. Description: Crystal Structure of Mei2 RRM3 Category: Unreleased Structures [[Category: Shen, S.Y...

OCA — Wed, 07 Apr 2021 04:32:40 GMT

New page: '''Unreleased structure''' The entry 7eio is ON HOLD Authors: Shen, S.Y., Li, F.D. Description: Crystal Structure of Mei2 RRM3 Category: Unreleased Structures [[Category: Shen, S.Y...

New page

'''Unreleased structure'''

The entry 7eio is ON HOLD

Authors: Shen, S.Y., Li, F.D.

Description: Crystal Structure of Mei2 RRM3
[[Category: Unreleased Structures]]
[[Category: Shen, S.Y]]
[[Category: Li, F.D]]