OCA at 16:55, 29 November 2023

OCA — Wed, 29 Nov 2023 16:55:59 GMT

←Older revision		Revision as of 16:55, 29 November 2023
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-	====	+	==Crystal structure of HpaR-DNA complex from Acinetobacter baumannii==
-	<StructureSection load='7el3' size='340' side='right'caption='[[7el3]]' scene=''>	+	<StructureSection load='7el3' size='340' side='right'caption='[[7el3]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7el3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii] and [https://en.wikipedia.org/wiki/Acinetobacter_baumannii_ZW85-1 Acinetobacter baumannii ZW85-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EL3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EL3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7el3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7el3 OCA], [https://pdbe.org/7el3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7el3 RCSB], [https://www.ebi.ac.uk/pdbsum/7el3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7el3 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7el3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7el3 OCA], [https://pdbe.org/7el3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7el3 RCSB], [https://www.ebi.ac.uk/pdbsum/7el3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7el3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A4Q4GPX4_ACIBA A0A4Q4GPX4_ACIBA]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HpaR is a transcription regulator in the MarR family that controls the expression of the gene cluster responsible for conversion of p-hydroxyphenylacetate to pyruvate and succinate for cellular metabolism. Here, we report the biochemical and structural characterization of Acinetobacter baumannii HpaR (AbHpaR) and its complex with cognate DNA. Our study revealed that AbHpaR binds upstream of the divergently transcribed hpaA gene and the meta-cleavage operon, as well as the hpaR gene, thereby repressing their transcription by blocking access of RNA polymerase. Structural analysis of AbHpaR-DNA complex revealed that the DNA binding specificity can be achieved via a combination of both direct and indirect DNA sequence readouts. DNA binding of AbHpaR is weakened by 3,4-dihydroxyphenylacetate (DHPA), which is the substrate of the meta-cleavage reactions; this likely leads to expression of the target genes. Based on our findings, we propose a model for how A. baumannii controls transcription of HPA-metabolizing genes, which highlights the independence of global catabolite repression and could be beneficial for metabolic engineering towards bioremediation applications.
		+
		+	Mechanism of transcription regulation by Acinetobacter baumannii HpaR in the catabolism of p-hydroxyphenylacetate.,Permsirivisarn P, Yuenyao A, Pramanpol N, Charoenwattanasatien R, Suginta W, Chaiyen P, Pakotiprapha D FEBS J. 2021 Dec 30. doi: 10.1111/febs.16340. PMID:34967505<ref>PMID:34967505</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7el3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acinetobacter baumannii]]
		+	[[Category: Acinetobacter baumannii ZW85-1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Pakotiprapha D]]
		+	[[Category: Permsirivisarn P]]

OCA at 07:27, 19 January 2022

OCA — Wed, 19 Jan 2022 07:27:19 GMT

←Older revision		Revision as of 07:27, 19 January 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7el3 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7el3' size='340' side='right'caption='[[7el3]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7el3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7el3 OCA], [https://pdbe.org/7el3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7el3 RCSB], [https://www.ebi.ac.uk/pdbsum/7el3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7el3 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:17, 5 May 2021

OCA — Wed, 05 May 2021 07:17:04 GMT

←Older revision		Revision as of 07:17, 5 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7el3 is ON HOLD	+	The entry 7el3 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7el3" [edit=sysop:move=sysop]

OCA — Wed, 14 Apr 2021 05:36:41 GMT

Protected "7el3" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:36, 14 April 2021

OCA: New page: '''Unreleased structure''' The entry 7el3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Apr 2021 05:36:40 GMT

New page: '''Unreleased structure''' The entry 7el3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7el3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7el3 - Revision history

OCA at 16:55, 29 November 2023

OCA at 07:27, 19 January 2022

OCA at 07:17, 5 May 2021

OCA: Protected "7el3" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7el3 is ON HOLD Authors: Description: Category: Unreleased Structures