7eqi - Revision history

OCA at 16:59, 29 November 2023

OCA — Wed, 29 Nov 2023 16:59:50 GMT

←Older revision		Revision as of 16:59, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7eqi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EQI FirstGlance]. <br>		<table><tr><td colspan='2'>[[7eqi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EQI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqi OCA], [https://pdbe.org/7eqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqi RCSB], [https://www.ebi.ac.uk/pdbsum/7eqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqi ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqi OCA], [https://pdbe.org/7eqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqi RCSB], [https://www.ebi.ac.uk/pdbsum/7eqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 07:32, 3 November 2022

OCA — Thu, 03 Nov 2022 07:32:04 GMT

←Older revision		Revision as of 07:32, 3 November 2022
Line 1:		Line 1:

	==ChlB3 [Aceyltransferase]==		==ChlB3 [Aceyltransferase]==
-	<StructureSection load='7eqi' size='340' side='right'caption='[[7eqi]]' scene=''>	+	<StructureSection load='7eqi' size='340' side='right'caption='[[7eqi]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EQI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eqi]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EQI FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqi OCA], [https://pdbe.org/7eqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqi RCSB], [https://www.ebi.ac.uk/pdbsum/7eqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqi ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqi OCA], [https://pdbe.org/7eqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqi RCSB], [https://www.ebi.ac.uk/pdbsum/7eqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q0R4P5_STRAT Q0R4P5_STRAT]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chlorothricin (CHL) belongs to a spirotetronate antibiotic family produced by Streptomyces antibioticus that inhibits pyruvate carboxylase and malate dehydrogenase. For the biosynthesis of CHL, ChlB3 plays a crucial role by introducing the 6-methylsalicylic acid (6MSA) moiety to ChlB2, an acyl carrier protein (ACP). However, the structural insight and catalytic mechanism of ChlB3 was unclear. In the current study, the crystal structure of ChlB3 was solved at 3.1 A-resolution and a catalytic mechanism was proposed on the basis of conserved residues of structurally related enzymes. ChlB3 is a dimer having the same active sites as CerJ (a structural homologous enzyme) and uses a KSIII-like fold to work as an acyltransferase. The relaxed substrate specificity of ChlB3 was defined by its catalytic efficiencies (kcat/Km) for non-ACP tethered synthetic substrates such as 6MSA-SNAC, acetyl-SNAC, and cyclohexonyl-SNAC. ChlB3 successfully detached the 6MSA moiety from 6MSA-SNAC substrate and this hydrolytic activity demonstrated that ChlB3 has the potential to catalyze non-ACP tethered substrates. Structural comparison indicated that ChlB3 belongs to FabH family and showed 0.6-2.5 A root mean square deviation (RMSD) with structural homologous enzymes. Molecular docking and dynamics simulations were implemented to understand substrate active site and structural behavior such as the open and closed conformation of the ChlB3 protein. The resultant catalytic and substrate recognition mechanism suggested that ChlB3 has the potential to use non-native substrates and minimize the labor of expressing ACP protein. This versatile acyltransferase activity may pave the way for manufacturing CHL variants and may help to hydrolyze several thioester-based compounds.
		+
		+	Structural Insight of KSIII (beta-Ketoacyl-ACP Synthase)-like Acyltransferase ChlB3 in the Biosynthesis of Chlorothricin.,Saeed AU, Rahman MU, Chen HF, Zheng J Molecules. 2022 Sep 28;27(19). pii: molecules27196405. doi:, 10.3390/molecules27196405. PMID:36234941<ref>PMID:36234941</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eqi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces antibioticus]]
	[[Category: Saeed AU]]		[[Category: Saeed AU]]
	[[Category: Zheng J]]		[[Category: Zheng J]]

OCA at 12:13, 9 June 2021

OCA — Wed, 09 Jun 2021 12:13:39 GMT

←Older revision		Revision as of 12:13, 9 June 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7eqi ~~is ON HOLD~~	+	==ChlB3 [Aceyltransferase]==
-		+	<StructureSection load='7eqi' size='340' side='right'caption='[[7eqi]]' scene=''>
-	~~Authors~~: ~~Saeed~~, A.U., ~~Zheng~~, J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EQI FirstGlance]. <br>
-	~~Description~~: ~~ChlB3~~ [~~Aceyltransferase~~]	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqi OCA], [https://pdbe.org/7eqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqi RCSB], [https://www.ebi.ac.uk/pdbsum/7eqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Saeed~~, A.U~~]]	+	__TOC__
-	[[Category: Zheng, J]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Saeed AU]]
		+	[[Category: Zheng J]]

OCA: Protected "7eqi" [edit=sysop:move=sysop]

OCA — Wed, 12 May 2021 08:04:10 GMT

Protected "7eqi" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:04, 12 May 2021

OCA: New page: '''Unreleased structure''' The entry 7eqi is ON HOLD Authors: Saeed, A.U., Zheng, J. Description: ChlB3 [Aceyltransferase] Category: Unreleased Structures Category: Saeed, A.U ...

OCA — Wed, 12 May 2021 08:04:09 GMT

New page: '''Unreleased structure''' The entry 7eqi is ON HOLD Authors: Saeed, A.U., Zheng, J. Description: ChlB3 [Aceyltransferase] Category: Unreleased Structures Category: Saeed, A.U ...

New page

'''Unreleased structure'''

The entry 7eqi is ON HOLD

Authors: Saeed, A.U., Zheng, J.

Description: ChlB3 [Aceyltransferase]
[[Category: Unreleased Structures]]
[[Category: Saeed, A.U]]
[[Category: Zheng, J]]