7eqx - Revision history

OCA at 17:00, 29 November 2023

OCA — Wed, 29 Nov 2023 17:00:16 GMT

←Older revision		Revision as of 17:00, 29 November 2023
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-	====	+	==Crystal structure of an Aedes aegypti procarboxypeptidase B1==
-	<StructureSection load='7eqx' size='340' side='right'caption='[[7eqx]]' scene=''>	+	<StructureSection load='7eqx' size='340' side='right'caption='[[7eqx]], [[Resolution\|resolution]] 2.08Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eqx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aedes_aegypti Aedes aegypti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EQX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqx OCA], [https://pdbe.org/7eqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqx RCSB], [https://www.ebi.ac.uk/pdbsum/7eqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqx ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.08Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqx OCA], [https://pdbe.org/7eqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqx RCSB], [https://www.ebi.ac.uk/pdbsum/7eqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1S4F020_AEDAE A0A1S4F020_AEDAE]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metallocarboxypeptidases play critical roles in the development of mosquitoes and influence pathogen/parasite infection of the mosquito midgut. Here, we report the crystal structure of Aedes aegypti procarboxypeptidase B1 (PCPBAe1), characterized its substrate specificity and mechanism of binding to and inhibiting Dengue virus (DENV). We show that the activated PCPBAe1 (CPBAe1) hydrolyzes both Arg- and Lys-substrates, which is modulated by residues Asp(251) and Ser(239) Notably, these residues are conserved in CPBs across mosquito species, possibly required for efficient digestion of basic dietary residues that are necessary for mosquito reproduction and development. Importantly, we characterized the interaction between PCPBAe1 and DENV envelope (E) protein, virus-like particles, and infectious virions. We identified residues Asp(18A), Glu(19A), Glu(85), Arg(87), and Arg(89) of PCPBAe1 are essential for interaction with DENV. PCPBAe1 maps to the dimeric interface of the E protein domains I/II (Lys(64)-Glu(84), Val(238)-Val(252), and Leu(278)-Leu(287)). Overall, our studies provide general insights into how the substrate-binding property of mosquito carboxypeptidases could be targeted to potentially control mosquito populations or proposes a mechanism by which PCPBAe1 binds to and inhibits DENV.
		+
		+	Structure of Aedes aegypti procarboxypeptidase B1 and its binding with Dengue virus for controlling infection.,Gavor E, Choong YK, Tulsian NK, Nayak D, Idris F, Sivaraman H, Ting DHR, Sylvie A, Mok YK, Kini RM, Sivaraman J Life Sci Alliance. 2021 Nov 8;5(1):e202101211. doi: 10.26508/lsa.202101211. Print , 2022 Jan. PMID:34750241<ref>PMID:34750241</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eqx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aedes aegypti]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Choong YK]]
		+	[[Category: Gavor E]]
		+	[[Category: Jobichen C]]
		+	[[Category: Sivaraman J]]

OCA at 06:14, 10 November 2021

OCA — Wed, 10 Nov 2021 06:14:27 GMT

←Older revision		Revision as of 06:14, 10 November 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7eqx ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7eqx' size='340' side='right'caption='[[7eqx]]' scene=''>
-	~~Authors~~: ~~Choong, Y~~.K.~~, Gavor, E~~.~~, Jobichen, C~~.~~, Sivaraman, J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of an Aedes aegypti procarboxypeptidase B1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eqx OCA], [https://pdbe.org/7eqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eqx RCSB], [https://www.ebi.ac.uk/pdbsum/7eqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eqx ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Jobichen~~, C]]	+	__TOC__
-	[~~[Category~~: ~~Gavor, E~~]]	+	</StructureSection>
-	[[Category: ~~Sivaraman, J~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Choong, Y.K~~]]	+	[[Category: Z-disk]]

OCA at 10:56, 9 June 2021

OCA — Wed, 09 Jun 2021 10:56:22 GMT

←Older revision		Revision as of 10:56, 9 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eqx is ON HOLD	+	The entry 7eqx is ON HOLD until Paper Publication

	Authors: Choong, Y.K., Gavor, E., Jobichen, C., Sivaraman, J.		Authors: Choong, Y.K., Gavor, E., Jobichen, C., Sivaraman, J.

OCA: Protected "7eqx" [edit=sysop:move=sysop]

OCA — Wed, 12 May 2021 08:05:07 GMT

Protected "7eqx" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:05, 12 May 2021

OCA: New page: '''Unreleased structure''' The entry 7eqx is ON HOLD Authors: Choong, Y.K., Gavor, E., Jobichen, C., Sivaraman, J. Description: Crystal structure of an Aedes aegypti procarboxypeptidas...

OCA — Wed, 12 May 2021 08:05:06 GMT

New page: '''Unreleased structure''' The entry 7eqx is ON HOLD Authors: Choong, Y.K., Gavor, E., Jobichen, C., Sivaraman, J. Description: Crystal structure of an Aedes aegypti procarboxypeptidas...

New page

'''Unreleased structure'''

The entry 7eqx is ON HOLD

Authors: Choong, Y.K., Gavor, E., Jobichen, C., Sivaraman, J.

Description: Crystal structure of an Aedes aegypti procarboxypeptidase B1
[[Category: Unreleased Structures]]
[[Category: Jobichen, C]]
[[Category: Gavor, E]]
[[Category: Sivaraman, J]]
[[Category: Choong, Y.K]]