7ery - Revision history

OCA at 07:11, 3 April 2024

OCA — Wed, 03 Apr 2024 07:11:51 GMT

←Older revision		Revision as of 07:11, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='7ery' size='340' side='right'caption='[[7ery]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='7ery' size='340' side='right'caption='[[7ery]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ery]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ERY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ERY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ery]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ERY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ERY FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max~~-~~height: 3em;'>~~[[~~7erx~~\|~~7erx~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ery OCA], [https://pdbe.org/7ery PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ery RCSB], [https://www.ebi.ac.uk/pdbsum/7ery PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ery ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ery OCA], [https://pdbe.org/7ery PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ery RCSB], [https://www.ebi.ac.uk/pdbsum/7ery PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ery ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q0DPB7_ORYSJ Q0DPB7_ORYSJ]
-	Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, the most desirable steviol glycosides Reb D and Reb M, devoid of unpleasant aftertaste, are naturally produced only in trace amounts due to low levels of specific beta (1-2) glucosylation in Stevia. Here, we report the biochemical and structural characterization of OsUGT91C1, a glycosyltransferase from Oryza sativa, which is efficient at catalyzing beta (1-2) glucosylation. The enzyme's ability to bind steviol glycoside substrate in three modes underlies its flexibility to catalyze beta (1-2) glucosylation in two distinct orientations as well as beta (1-6) glucosylation. Guided by the structural insights, we engineer this enzyme to enhance the desirable beta (1-2) glucosylation, eliminate beta (1-6) glucosylation, and obtain a promising catalyst for the industrial production of naturally rare but palatable steviol glycosides.	+

-	Catalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.,Zhang J, Tang M, Chen Y, Ke D, Zhou J, Xu X, Yang W, He J, Dong H, Wei Y, Naismith JH, Lin Y, Zhu X, Cheng W Nat Commun. 2021 Dec 2;12(1):7030. doi: 10.1038/s41467-021-27144-4. PMID:34857750<ref>PMID:34857750</ref>	+	==See Also==
-		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7ery" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Zhu, X~~]]	+	[[Category: Oryza sativa Japonica Group]]
-	[[Category: ~~Glycosyltransferase apo form]]~~	+	[[Category: Zhu X]]
-	~~[[Category: Transferase~~]]	+

OCA at 16:56, 15 December 2021

OCA — Wed, 15 Dec 2021 16:56:31 GMT

←Older revision		Revision as of 16:56, 15 December 2021
Line 1:		Line 1:

	==apo form of the glycosyltransferase==		==apo form of the glycosyltransferase==
-	<StructureSection load='7ery' size='340' side='right'caption='[[7ery]]' scene=''>	+	<StructureSection load='7ery' size='340' side='right'caption='[[7ery]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ERY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ERY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ery]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ERY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ERY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ery OCA], [https://pdbe.org/7ery PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ery RCSB], [https://www.ebi.ac.uk/pdbsum/7ery PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ery ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7erx\|7erx]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ery OCA], [https://pdbe.org/7ery PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ery RCSB], [https://www.ebi.ac.uk/pdbsum/7ery PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ery ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, the most desirable steviol glycosides Reb D and Reb M, devoid of unpleasant aftertaste, are naturally produced only in trace amounts due to low levels of specific beta (1-2) glucosylation in Stevia. Here, we report the biochemical and structural characterization of OsUGT91C1, a glycosyltransferase from Oryza sativa, which is efficient at catalyzing beta (1-2) glucosylation. The enzyme's ability to bind steviol glycoside substrate in three modes underlies its flexibility to catalyze beta (1-2) glucosylation in two distinct orientations as well as beta (1-6) glucosylation. Guided by the structural insights, we engineer this enzyme to enhance the desirable beta (1-2) glucosylation, eliminate beta (1-6) glucosylation, and obtain a promising catalyst for the industrial production of naturally rare but palatable steviol glycosides.
		+
		+	Catalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.,Zhang J, Tang M, Chen Y, Ke D, Zhou J, Xu X, Yang W, He J, Dong H, Wei Y, Naismith JH, Lin Y, Zhu X, Cheng W Nat Commun. 2021 Dec 2;12(1):7030. doi: 10.1038/s41467-021-27144-4. PMID:34857750<ref>PMID:34857750</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ery" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Zhu X]]	+	[[Category: Zhu, X]]
		+	[[Category: Glycosyltransferase apo form]]
		+	[[Category: Transferase]]

OCA at 09:55, 8 December 2021

OCA — Wed, 08 Dec 2021 09:55:12 GMT

←Older revision		Revision as of 09:55, 8 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ery ~~is ON HOLD until May 08 2023~~	+	==apo form of the glycosyltransferase==
-		+	<StructureSection load='7ery' size='340' side='right'caption='[[7ery]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ERY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ERY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ery FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ery OCA], [https://pdbe.org/7ery PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ery RCSB], [https://www.ebi.ac.uk/pdbsum/7ery PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ery ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Zhu X]]

OCA at 07:48, 26 May 2021

OCA — Wed, 26 May 2021 07:48:34 GMT

←Older revision		Revision as of 07:48, 26 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ery is ON HOLD	+	The entry 7ery is ON HOLD until May 08 2023

	Authors:		Authors:

OCA: Protected "7ery" [edit=sysop:move=sysop]

OCA — Wed, 19 May 2021 08:43:08 GMT

Protected "7ery" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 19 May 2021

OCA: New page: '''Unreleased structure''' The entry 7ery is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 May 2021 08:43:06 GMT

New page: '''Unreleased structure''' The entry 7ery is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ery is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]