7es1 - Revision history

OCA at 07:11, 3 April 2024

OCA — Wed, 03 Apr 2024 07:11:51 GMT

←Older revision		Revision as of 07:11, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='7es1' size='340' side='right'caption='[[7es1]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='7es1' size='340' side='right'caption='[[7es1]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7es1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7es1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=JDF:steviol~~-~~19-o-glucoside'>JDF</scene>~~, ~~<scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.66Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[7erx\|7erx]]~~, ~~[[7ery\|7ery]], [[7er0\|7er0]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JDF:steviol-19-o-glucoside'>JDF</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es1 OCA], [https://pdbe.org/7es1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es1 RCSB], [https://www.ebi.ac.uk/pdbsum/7es1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es1 OCA], [https://pdbe.org/7es1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es1 RCSB], [https://www.ebi.ac.uk/pdbsum/7es1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es1 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q0DPB7_ORYSJ Q0DPB7_ORYSJ]
-	Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, the most desirable steviol glycosides Reb D and Reb M, devoid of unpleasant aftertaste, are naturally produced only in trace amounts due to low levels of specific beta (1-2) glucosylation in Stevia. Here, we report the biochemical and structural characterization of OsUGT91C1, a glycosyltransferase from Oryza sativa, which is efficient at catalyzing beta (1-2) glucosylation. The enzyme's ability to bind steviol glycoside substrate in three modes underlies its flexibility to catalyze beta (1-2) glucosylation in two distinct orientations as well as beta (1-6) glucosylation. Guided by the structural insights, we engineer this enzyme to enhance the desirable beta (1-2) glucosylation, eliminate beta (1-6) glucosylation, and obtain a promising catalyst for the industrial production of naturally rare but palatable steviol glycosides.	+

-	Catalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.,Zhang J, Tang M, Chen Y, Ke D, Zhou J, Xu X, Yang W, He J, Dong H, Wei Y, Naismith JH, Lin Y, Zhu X, Cheng W Nat Commun. 2021 Dec 2;12(1):7030. doi: 10.1038/s41467-021-27144-4. PMID:34857750<ref>PMID:34857750</ref>	+	==See Also==
-		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7es1" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Zhu, X~~]]	+	[[Category: Oryza sativa Japonica Group]]
-	[[Category: ~~A rice glycosyltransferase]]~~	+	[[Category: Zhu X]]
-	~~[[Category: Transferase~~]]	+

OCA at 16:56, 15 December 2021

OCA — Wed, 15 Dec 2021 16:56:41 GMT

←Older revision		Revision as of 16:56, 15 December 2021
Line 1:		Line 1:

	==glycosyltransferase in complex with UDP and ST==		==glycosyltransferase in complex with UDP and ST==
-	<StructureSection load='7es1' size='340' side='right'caption='[[7es1]]' scene=''>	+	<StructureSection load='7es1' size='340' side='right'caption='[[7es1]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7es1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es1 OCA], [https://pdbe.org/7es1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es1 RCSB], [https://www.ebi.ac.uk/pdbsum/7es1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es1 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JDF:steviol-19-o-glucoside'>JDF</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7erx\|7erx]], [[7ery\|7ery]], [[7er0\|7er0]]</div></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es1 OCA], [https://pdbe.org/7es1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es1 RCSB], [https://www.ebi.ac.uk/pdbsum/7es1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es1 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, the most desirable steviol glycosides Reb D and Reb M, devoid of unpleasant aftertaste, are naturally produced only in trace amounts due to low levels of specific beta (1-2) glucosylation in Stevia. Here, we report the biochemical and structural characterization of OsUGT91C1, a glycosyltransferase from Oryza sativa, which is efficient at catalyzing beta (1-2) glucosylation. The enzyme's ability to bind steviol glycoside substrate in three modes underlies its flexibility to catalyze beta (1-2) glucosylation in two distinct orientations as well as beta (1-6) glucosylation. Guided by the structural insights, we engineer this enzyme to enhance the desirable beta (1-2) glucosylation, eliminate beta (1-6) glucosylation, and obtain a promising catalyst for the industrial production of naturally rare but palatable steviol glycosides.
		+
		+	Catalytic flexibility of rice glycosyltransferase OsUGT91C1 for the production of palatable steviol glycosides.,Zhang J, Tang M, Chen Y, Ke D, Zhou J, Xu X, Yang W, He J, Dong H, Wei Y, Naismith JH, Lin Y, Zhu X, Cheng W Nat Commun. 2021 Dec 2;12(1):7030. doi: 10.1038/s41467-021-27144-4. PMID:34857750<ref>PMID:34857750</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7es1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Zhu X]]	+	[[Category: Zhu, X]]
		+	[[Category: A rice glycosyltransferase]]
		+	[[Category: Transferase]]

OCA at 09:55, 8 December 2021

OCA — Wed, 08 Dec 2021 09:55:13 GMT

←Older revision		Revision as of 09:55, 8 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7es1 ~~is ON HOLD until May 08 2023~~	+	==glycosyltransferase in complex with UDP and ST==
-		+	<StructureSection load='7es1' size='340' side='right'caption='[[7es1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ES1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ES1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7es1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7es1 OCA], [https://pdbe.org/7es1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7es1 RCSB], [https://www.ebi.ac.uk/pdbsum/7es1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7es1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Zhu X]]

OCA at 13:22, 2 June 2021

OCA — Wed, 02 Jun 2021 13:22:21 GMT

←Older revision		Revision as of 13:22, 2 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7es1 is ON HOLD	+	The entry 7es1 is ON HOLD until May 08 2023

	Authors:		Authors:

OCA: Protected "7es1" [edit=sysop:move=sysop]

OCA — Wed, 19 May 2021 08:43:22 GMT

Protected "7es1" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:43, 19 May 2021

OCA: New page: '''Unreleased structure''' The entry 7es1 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 19 May 2021 08:43:20 GMT

New page: '''Unreleased structure''' The entry 7es1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7es1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]