7eun - Revision history

OCA at 17:04, 29 November 2023

OCA — Wed, 29 Nov 2023 17:04:16 GMT

←Older revision		Revision as of 17:04, 29 November 2023
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	<StructureSection load='7eun' size='340' side='right'caption='[[7eun]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='7eun' size='340' side='right'caption='[[7eun]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7eun]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eun]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_lavendulae Streptomyces lavendulae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/N(omega)-hydroxy-L-arginine_amidinohydrolase N(omega)-hydroxy-L-arginine amidinohydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.25 3.5.3.25] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eun OCA], [https://pdbe.org/7eun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eun RCSB], [https://www.ebi.ac.uk/pdbsum/7eun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eun ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eun OCA], [https://pdbe.org/7eun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eun RCSB], [https://www.ebi.ac.uk/pdbsum/7eun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eun ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DCSB_STRLA DCSB_STRLA]] Involved in the biosynthesis of the antibiotic D-cycloserine (DCS), a cyclic structural analog of D-alanine, used as an antitubercular agent. Catalyzes the hydrolysis of N(omega)-hydroxy-L-arginine (NHA) to yield hydroxyurea (HU) and L-ornithine.<ref>PMID:20086163</ref> <ref>PMID:22547619</ref>	+	[https://www.uniprot.org/uniprot/DCSB_STRLA DCSB_STRLA] Involved in the biosynthesis of the antibiotic D-cycloserine (DCS), a cyclic structural analog of D-alanine, used as an antitubercular agent. Catalyzes the hydrolysis of N(omega)-hydroxy-L-arginine (NHA) to yield hydroxyurea (HU) and L-ornithine.<ref>PMID:20086163</ref> <ref>PMID:22547619</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Matoba, Y~~]]	+	[[Category: Streptomyces lavendulae]]
-	[[Category: ~~Oda, K~~]]	+	[[Category: Matoba Y]]
-	[[Category: ~~Antibiotic]]~~	+	[[Category: Oda K]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 05:16, 15 June 2022

OCA — Wed, 15 Jun 2022 05:16:31 GMT

←Older revision		Revision as of 05:16, 15 June 2022
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	==Crystal structure of N(omega)-hydroxy-L-arginine hydrolase in complex with ABH==		==Crystal structure of N(omega)-hydroxy-L-arginine hydrolase in complex with ABH==
-	<StructureSection load='7eun' size='340' side='right'caption='[[7eun]]' scene=''>	+	<StructureSection load='7eun' size='340' side='right'caption='[[7eun]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eun]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eun OCA], [https://pdbe.org/7eun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eun RCSB], [https://www.ebi.ac.uk/pdbsum/7eun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eun ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/N(omega)-hydroxy-L-arginine_amidinohydrolase N(omega)-hydroxy-L-arginine amidinohydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.25 3.5.3.25] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eun OCA], [https://pdbe.org/7eun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eun RCSB], [https://www.ebi.ac.uk/pdbsum/7eun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eun ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/DCSB_STRLA DCSB_STRLA]] Involved in the biosynthesis of the antibiotic D-cycloserine (DCS), a cyclic structural analog of D-alanine, used as an antitubercular agent. Catalyzes the hydrolysis of N(omega)-hydroxy-L-arginine (NHA) to yield hydroxyurea (HU) and L-ornithine.<ref>PMID:20086163</ref> <ref>PMID:22547619</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	DcsB, an enzyme produced from the d-cycloserine biosynthetic gene cluster, displays moderate similarity to arginase in the sequence and three-dimensional structure. Arginase is a ubiquitous enzyme hydrolyzing l-arginine to generate l-ornithine and urea, whereas DcsB hydrolyzes N(omega) -hydroxy-l-arginine (l-NOHA), an arginase inhibitor, to generate l-ornithine and hydroxyurea. We determined the crystal structure of DcsB associated with l-ornithine and that with the tetrahedral derivative of 2(S)-amino-6-boronohexanoic acid, whose boron atom forms a covalent bond with an oxygen atom bridging two manganese ions at the active center. The substrate-binding pocket of DcsB is narrower than that of arginase, suggesting that DcsB is unsuitable for the binding of l-NOHA in an inhibitory manner. The transition state-like structure demonstrated that Asp210 and Glu241 have a role to trap a positively charged ion near the dimanganese cluster. Kinetic analysis using the mutated DcsB showed that the enzyme employs different catalytic mechanisms under the neutral and alkaline pH conditions. Glu241 in DcsB is likely involved in the recognition of the hydroxyguanidino group of l-NOHA, whereas Asp210, in cooperation with Glu241, seems to contribute to the reactivity toward the protonated l-NOHA, which is a preferable species under the neutral pH conditions. After entering of the protonated l-NOHA to the substrate-binding pocket of DcsB, a hydronium ion may be trapped at the positive ion-binding site. Then, the ion serves as a specific acid catalyst to facilitate the collapse of the tetrahedral intermediate of l-NOHA.
		+
		+	Catalytic mechanism of DcsB: Arginase framework used for hydrolyzing its inhibitor.,Oda K, Sakaguchi T, Matoba Y Protein Sci. 2022 Jun;31(6):e4338. doi: 10.1002/pro.4338. PMID:35634777<ref>PMID:35634777</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eun" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Matoba Y]]	+	[[Category: Matoba, Y]]
-	[[Category: Oda K]]	+	[[Category: Oda, K]]
		+	[[Category: Antibiotic]]
		+	[[Category: Hydrolase]]

OCA at 10:14, 18 May 2022

OCA — Wed, 18 May 2022 10:14:15 GMT

←Older revision		Revision as of 10:14, 18 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7eun ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of N(omega)-hydroxy-L-arginine hydrolase in complex with ABH==
-		+	<StructureSection load='7eun' size='340' side='right'caption='[[7eun]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUN FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eun OCA], [https://pdbe.org/7eun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eun RCSB], [https://www.ebi.ac.uk/pdbsum/7eun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eun ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Matoba Y]]
		+	[[Category: Oda K]]

OCA at 08:58, 6 April 2022

OCA — Wed, 06 Apr 2022 08:58:17 GMT

←Older revision		Revision as of 08:58, 6 April 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eun is ON HOLD	+	The entry 7eun is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:54, 16 March 2022

OCA — Wed, 16 Mar 2022 04:54:48 GMT

←Older revision		Revision as of 04:54, 16 March 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eun is ON HOLD ~~until Paper Publication~~	+	The entry 7eun is ON HOLD

	Authors:		Authors:

OCA: Protected "7eun" [edit=sysop:move=sysop]

OCA — Wed, 26 May 2021 07:51:15 GMT

Protected "7eun" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:51, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7eun is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 May 2021 07:51:14 GMT

New page: '''Unreleased structure''' The entry 7eun is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7eun is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]