7euu - Revision history

OCA at 17:04, 29 November 2023

OCA — Wed, 29 Nov 2023 17:04:32 GMT

←Older revision		Revision as of 17:04, 29 November 2023
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	==Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) in complex with N-oxalylglycine and pre-cercosporin==		==Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) in complex with N-oxalylglycine and pre-cercosporin==
-	<StructureSection load='7euu' size='340' side='right'caption='[[7euu]]' scene=''>	+	<StructureSection load='7euu' size='340' side='right'caption='[[7euu]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7euu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cercospora_sojina Cercospora sojina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7euu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7euu OCA], [https://pdbe.org/7euu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7euu RCSB], [https://www.ebi.ac.uk/pdbsum/7euu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7euu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.202Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=JD9:2,6,11-trimethoxy-4,7,9-tris(oxidanyl)-1,12-bis[(2R)-2-oxidanylpropyl]perylene-3,10-dione'>JD9</scene>, <scene name='pdbligand=OGA:N-OXALYLGLYCINE'>OGA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7euu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7euu OCA], [https://pdbe.org/7euu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7euu RCSB], [https://www.ebi.ac.uk/pdbsum/7euu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7euu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CTB9_CERBT CTB9_CERBT] Hydroxylase/desaturase; part of the gene cluster that mediates the biosynthesis of cercosporin, a light-activated, non-host-selective toxin (PubMed:29844193). The perylenequinone chromophore of cercosporin absorbs light energy to attain an electronically-activated triplet state and produces active oxygen species such as the hydroxyl radical, superoxide, hydrogen peroxide or singlet oxygen upon reaction with oxygen molecules (PubMed:11701851). These reactive oxygen species cause damage to various cellular components including lipids, proteins and nucleic acids (PubMed:11701851). The first step of cercosporin biosynthesis is performed by the polyketide synthase CTB1 which catalyzes the formation of nor-toralactone (Probable). The starter unit acyltransferase (SAT) domain of CTB1 initiates polyketide extension by the selective utilization of acetyl-CoA, which is elongated to the heptaketide in the beta-ketoacyl synthase (KS) domain by successive condensations with six malonyl units introduced by the malonyl acyltransferase (MAT) domain. The product template (PT) domain catalyzes C4-C9 and C2-C11 aldol cyclizations and dehydrations to a trihydroxynaphthalene, which is thought to be delivered to the thioesterase (TE) domain for product release (Probable). The bifunctional enzyme CTB3 then methylates nor-toralactone to toralactone before conducting an unusual oxidative aromatic ring opening (Probable). The O-methyltransferase CTB2 further methylates the nascent OH-6 of the CBT3 product, blocking further oxidation at this site before the reductase CTB6 reduces the 2-oxopropyl ketone at position C7, giving naphthalene (Probable). The FAD-dependent monooxygenase CTB5 in concert with the multicopper oxidase CTB12 are responsible for homodimerization of naphthalene with CTB7 installing the dioxepine moiety, finally producing cercosporin (Probable). The fasciclin domain-containing protein CTB11 might act with CTB5 and CTB12 whereas the roles of CTB9 and CTB10 have still to be elucidated (By similarity).[UniProtKB:Q0UHZ9]<ref>PMID:29844193</ref> <ref>PMID:11701851</ref> <ref>PMID:29844193</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cercospora sojina]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hou XD]]		[[Category: Hou XD]]

OCA at 07:56, 25 May 2022

OCA — Wed, 25 May 2022 07:56:10 GMT

←Older revision		Revision as of 07:56, 25 May 2022
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-	'''Unreleased structure'''

-	~~The entry 7euu is ON HOLD until Paper Publication~~	+	==Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) in complex with N-oxalylglycine and pre-cercosporin==
-		+	<StructureSection load='7euu' size='340' side='right'caption='[[7euu]]' scene=''>
-	~~Authors: Hou, X.D., Liu, X.Z., Yuan, Z.B., Rao, Y.J.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EUU FirstGlance]. <br>
-	~~Description:~~ Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) in complex with N-oxalylglycine and pre-cercosporin	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7euu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7euu OCA], [https://pdbe.org/7euu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7euu RCSB], [https://www.ebi.ac.uk/pdbsum/7euu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7euu ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Yuan~~, Z.B]]	+	__TOC__
-	[[Category: ~~Liu, X.Z~~]]	+	</StructureSection>
-	[[Category: Hou~~, X.D~~]]	+	[[Category: Large Structures]]
-	[[Category: Rao~~, Y.J~~]]	+	[[Category: Hou XD]]
		+	[[Category: Liu XZ]]
		+	[[Category: Rao YJ]]
		+	[[Category: Yuan ZB]]

OCA at 07:45, 21 July 2021

OCA — Wed, 21 Jul 2021 07:45:07 GMT

←Older revision		Revision as of 07:45, 21 July 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7euu is ON HOLD	+	The entry 7euu is ON HOLD until Paper Publication

	Authors: Hou, X.D., Liu, X.Z., Yuan, Z.B., Rao, Y.J.		Authors: Hou, X.D., Liu, X.Z., Yuan, Z.B., Rao, Y.J.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Yuan, Z.B]]		[[Category: Yuan, Z.B]]
		+	[[Category: Liu, X.Z]]
	[[Category: Hou, X.D]]		[[Category: Hou, X.D]]
	[[Category: Rao, Y.J]]		[[Category: Rao, Y.J]]
-	[[Category: Liu, X.Z]]

OCA: Protected "7euu" [edit=sysop:move=sysop]

OCA — Wed, 26 May 2021 07:51:48 GMT

Protected "7euu" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:51, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7euu is ON HOLD Authors: Hou, X.D., Liu, X.Z., Yuan, Z.B., Rao, Y.J. Description: Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9)...

OCA — Wed, 26 May 2021 07:51:47 GMT

New page: '''Unreleased structure''' The entry 7euu is ON HOLD Authors: Hou, X.D., Liu, X.Z., Yuan, Z.B., Rao, Y.J. Description: Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9)...

New page

'''Unreleased structure'''

The entry 7euu is ON HOLD

Authors: Hou, X.D., Liu, X.Z., Yuan, Z.B., Rao, Y.J.

Description: Crystal structures of 2-oxoglutarate dependent dioxygenase (CTB9) in complex with N-oxalylglycine and pre-cercosporin
[[Category: Unreleased Structures]]
[[Category: Yuan, Z.B]]
[[Category: Hou, X.D]]
[[Category: Rao, Y.J]]
[[Category: Liu, X.Z]]