7ewi - Revision history

OCA at 17:05, 29 November 2023

OCA — Wed, 29 Nov 2023 17:05:30 GMT

←Older revision		Revision as of 17:05, 29 November 2023
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	<StructureSection load='7ewi' size='340' side='right'caption='[[7ewi]], [[Resolution\|resolution]] 1.93Å' scene=''>		<StructureSection load='7ewi' size='340' side='right'caption='[[7ewi]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ewi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EWI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ewi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EWI FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.93Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ewi OCA], [https://pdbe.org/7ewi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ewi RCSB], [https://www.ebi.ac.uk/pdbsum/7ewi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ewi ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ewi OCA], [https://pdbe.org/7ewi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ewi RCSB], [https://www.ebi.ac.uk/pdbsum/7ewi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ewi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/L7PFJ6_STAAU L7PFJ6_STAAU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kang, S M~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Kim, D H~~]]	+	[[Category: Kang SM]]
-	[[Category: ~~Lee, B J~~]]	+	[[Category: Kim DH]]
-	[[Category: Lee~~, S J~~]]	+	[[Category: Lee BJ]]
-	[[Category: ~~Hydrolase rnase]]~~	+	[[Category: Lee SJ]]
-	~~[[Category: Toxin~~]]	+

OCA at 08:37, 23 February 2022

OCA — Wed, 23 Feb 2022 08:37:45 GMT

←Older revision		Revision as of 08:37, 23 February 2022
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	==Toxin protein from Staphylococcus aureus==		==Toxin protein from Staphylococcus aureus==
-	<StructureSection load='7ewi' size='340' side='right'caption='[[7ewi]]' scene=''>	+	<StructureSection load='7ewi' size='340' side='right'caption='[[7ewi]], [[Resolution\|resolution]] 1.93Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EWI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ewi]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EWI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ewi OCA], [https://pdbe.org/7ewi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ewi RCSB], [https://www.ebi.ac.uk/pdbsum/7ewi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ewi ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ewi OCA], [https://pdbe.org/7ewi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ewi RCSB], [https://www.ebi.ac.uk/pdbsum/7ewi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ewi ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Staphylococcus aureus is a notorious and globally distributed pathogenic bacterium. New strategies to develop novel antibiotics based on intrinsic bacterial toxin-antitoxin (TA) systems have been recently reported. Because TA systems are present only in bacteria and not in humans, these distinctive systems are attractive targets for developing antibiotics with new modes of action. S. aureus PemIK is a type II TA system, comprising the toxin protein PemK and the labile antitoxin protein PemI. Here, we determined the crystal structures of both PemK and the PemIK complex, in which PemK is neutralized by PemI. Our biochemical approaches, including fluorescence quenching and polarization assays, identified Glu20, Arg25, Thr48, Thr49, and Arg84 of PemK as being important for RNase function. Our study indicates that the active site and RNA-binding residues of PemK are covered by PemI, leading to unique conformational changes in PemK accompanied by repositioning of the loop between beta1 and beta2. These changes can interfere with RNA binding by PemK. Overall, PemK adopts particular open and closed forms for precise neutralization by PemI. This structural and functional information on PemIK will contribute to the discovery and development of novel antibiotics in the form of peptides or small molecules inhibiting direct binding between PemI and PemK.
		+
		+	Role of PemI in the Staphylococcus aureus PemIK toxin-antitoxin complex: PemI controls PemK by acting as a PemK loop mimic.,Kim DH, Kang SM, Baek SM, Yoon HJ, Jang DM, Kim HS, Lee SJ, Lee BJ Nucleic Acids Res. 2022 Feb 10. pii: 6511975. doi: 10.1093/nar/gkab1288. PMID:35141752<ref>PMID:35141752</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ewi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kang SM]]	+	[[Category: Kang, S M]]
-	[[Category: Kim DH]]	+	[[Category: Kim, D H]]
-	[[Category: Lee BJ]]	+	[[Category: Lee, B J]]
-	[[Category: Lee SJ]]	+	[[Category: Lee, S J]]
		+	[[Category: Hydrolase rnase]]
		+	[[Category: Toxin]]

OCA at 10:15, 16 February 2022

OCA — Wed, 16 Feb 2022 10:15:13 GMT

←Older revision		Revision as of 10:15, 16 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ewi is ~~ON HOLD until Paper Publication~~	+	==Toxin protein from Staphylococcus aureus==
-		+	<StructureSection load='7ewi' size='340' side='right'caption='[[7ewi]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EWI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ewi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ewi OCA], [https://pdbe.org/7ewi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ewi RCSB], [https://www.ebi.ac.uk/pdbsum/7ewi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ewi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kang SM]]
		+	[[Category: Kim DH]]
		+	[[Category: Lee BJ]]
		+	[[Category: Lee SJ]]

OCA at 13:30, 17 June 2021

OCA — Thu, 17 Jun 2021 13:30:28 GMT

←Older revision		Revision as of 13:30, 17 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ewi is ON HOLD	+	The entry 7ewi is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ewi" [edit=sysop:move=sysop]

OCA — Wed, 02 Jun 2021 13:24:15 GMT

Protected "7ewi" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:24, 2 June 2021

OCA: New page: '''Unreleased structure''' The entry 7ewi is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Jun 2021 13:24:14 GMT

New page: '''Unreleased structure''' The entry 7ewi is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ewi is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]