OCA at 17:06, 29 November 2023

OCA — Wed, 29 Nov 2023 17:06:30 GMT

←Older revision		Revision as of 17:06, 29 November 2023
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	==Crystal structure of leech hyaluronidase==		==Crystal structure of leech hyaluronidase==
-	<StructureSection load='7eyo' size='340' side='right'caption='[[7eyo]]' scene=''>	+	<StructureSection load='7eyo' size='340' side='right'caption='[[7eyo]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eyo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hirudo_nipponia Hirudo nipponia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyo OCA], [https://pdbe.org/7eyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyo RCSB], [https://www.ebi.ac.uk/pdbsum/7eyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyo OCA], [https://pdbe.org/7eyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyo RCSB], [https://www.ebi.ac.uk/pdbsum/7eyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LHYAL_HIRNI LHYAL_HIRNI]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hyaluronidases have attracted a great deal of interest in the field of medicine due to their fundamental roles in the breakdown of hyaluronan. However, little is known about the catalytic mechanism of the hyaluronate 3-glycanohydrolases. Here, we report the crystal structure and cleavage pattern of a leech hyaluronidase (LHyal), which hydrolyzes the beta-1,3-glycosidic bonds of hyaluronan. LHyal exhibits the typical structural features of glycoside hydrolase 79 family but contains a variable 'exo-pocket' loop where basic residues R102 and K103 are the structural determinants of hyaluronan binding. Through analysis of the hydrolysis of even- and odd-numbered hyaluronan oligosaccharides, we demonstrate that hexasaccharide is the shortest natural substrate, which can be cleaved from both the reducing and non-reducing ends to release disaccharides, and pentasaccharides are the smallest fragments for recognition and hydrolysis. These observations provide new insights into the degradation of hyaluronan and the evolutionary relationships of the GH79 family enzymes.
		+
		+	Structure and cleavage pattern of a hyaluronate 3-glycanohydrolase in the glycoside hydrolase 79 family.,Huang H, Hou X, Xu R, Deng Z, Wang Y, Du G, Rao Y, Chen J, Kang Z Carbohydr Polym. 2022 Feb 1;277:118838. doi: 10.1016/j.carbpol.2021.118838. Epub , 2021 Nov 2. PMID:34893255<ref>PMID:34893255</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eyo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Hyaluronidase 3D structures\|Hyaluronidase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hirudo nipponia]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hou XD]]		[[Category: Hou XD]]

OCA at 07:56, 25 May 2022

OCA — Wed, 25 May 2022 07:56:15 GMT

←Older revision		Revision as of 07:56, 25 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7eyo is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of leech hyaluronidase==
-		+	<StructureSection load='7eyo' size='340' side='right'caption='[[7eyo]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYO FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyo OCA], [https://pdbe.org/7eyo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyo RCSB], [https://www.ebi.ac.uk/pdbsum/7eyo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hou XD]]
		+	[[Category: Huang H]]
		+	[[Category: Kang Z]]
		+	[[Category: Rao YJ]]

OCA at 04:54, 18 August 2021

OCA — Wed, 18 Aug 2021 04:54:34 GMT

←Older revision		Revision as of 04:54, 18 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eyo is ON HOLD	+	The entry 7eyo is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7eyo" [edit=sysop:move=sysop]

OCA — Wed, 09 Jun 2021 10:59:25 GMT

Protected "7eyo" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 9 June 2021

OCA: New page: '''Unreleased structure''' The entry 7eyo is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Jun 2021 10:59:24 GMT

New page: '''Unreleased structure''' The entry 7eyo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7eyo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7eyo - Revision history

OCA at 17:06, 29 November 2023

OCA at 07:56, 25 May 2022

OCA at 04:54, 18 August 2021

OCA: Protected "7eyo" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7eyo is ON HOLD Authors: Description: Category: Unreleased Structures