7eyu - Revision history

OCA at 17:06, 29 November 2023

OCA — Wed, 29 Nov 2023 17:06:36 GMT

←Older revision		Revision as of 17:06, 29 November 2023
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	==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin D==		==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin D==
-	<StructureSection load='7eyu' size='340' side='right'caption='[[7eyu]]' scene=''>	+	<StructureSection load='7eyu' size='340' side='right'caption='[[7eyu]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7eyu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_sp. Aspergillus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyu OCA], [https://pdbe.org/7eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyu RCSB], [https://www.ebi.ac.uk/pdbsum/7eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0CC:Andiconin+D'>0CC</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=OGA:N-OXALYLGLYCINE'>OGA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyu OCA], [https://pdbe.org/7eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyu RCSB], [https://www.ebi.ac.uk/pdbsum/7eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A6J4CX17_9EURO A0A6J4CX17_9EURO]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Non-heme iron and alpha-ketoglutarate-dependent (Fe/alphaKG) oxygenases catalyze various oxidative biotransformations. Due to their catalytic flexibility and high efficiency, Fe/alphaKG oxygenases have attracted keen attention for their application as biocatalysts. Here, we report the biochemical and structural characterizations of the unusually promiscuous and catalytically versatile Fe/alphaKG oxygenase SptF, involved in the biosynthesis of fungal meroterpenoid emervaridones. The in vitro analysis revealed that SptF catalyzes several continuous oxidation reactions, including hydroxylation, desaturation, epoxidation, and skeletal rearrangement. SptF exhibits extremely broad substrate specificity toward various meroterpenoids, and efficiently produced unique cyclopropane-ring-fused 5/3/5/5/6/6 and 5/3/6/6/6 scaffolds from terretonins. Moreover, SptF also hydroxylates steroids, including androsterone, testosterone, and progesterone, with different regiospecificities. Crystallographic and structure-based mutagenesis studies of SptF revealed the molecular basis of the enzyme reactions, and suggested that the malleability of the loop region contributes to the remarkable substrate promiscuity. SptF exhibits great potential as a promising biocatalyst for oxidation reactions.
		+
		+	Molecular insights into the unusually promiscuous and catalytically versatile Fe(II)/alpha-ketoglutarate-dependent oxygenase SptF.,Tao H, Mori T, Chen H, Lyu S, Nonoyama A, Lee S, Abe I Nat Commun. 2022 Jan 10;13(1):95. doi: 10.1038/s41467-021-27636-3. PMID:35013177<ref>PMID:35013177</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7eyu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dioxygenase 3D structures\|Dioxygenase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspergillus sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Abe I]]		[[Category: Abe I]]
	[[Category: Mori T]]		[[Category: Mori T]]
	[[Category: Tao H]]		[[Category: Tao H]]

OCA at 02:51, 21 April 2022

OCA — Thu, 21 Apr 2022 02:51:14 GMT

←Older revision		Revision as of 02:51, 21 April 2022
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-	'''Unreleased structure'''

-	~~The entry 7eyu is ON HOLD until Paper Publication~~	+	==Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin D==
-		+	<StructureSection load='7eyu' size='340' side='right'caption='[[7eyu]]' scene=''>
-	~~Authors: Tao, H., Mori, T., Abe, I.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EYU FirstGlance]. <br>
-	~~Description:~~ Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin D	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eyu OCA], [https://pdbe.org/7eyu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eyu RCSB], [https://www.ebi.ac.uk/pdbsum/7eyu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eyu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Abe, I]]	+	__TOC__
-	[[Category: Mori, T]]	+	</StructureSection>
-	[[Category: Tao, H]]	+	[[Category: Large Structures]]
		+	[[Category: Abe I]]
		+	[[Category: Mori T]]
		+	[[Category: Tao H]]

OCA at 07:33, 29 September 2021

OCA — Wed, 29 Sep 2021 07:33:32 GMT

←Older revision		Revision as of 07:33, 29 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7eyu is ON HOLD	+	The entry 7eyu is ON HOLD until Paper Publication

	Authors: Tao, H., Mori, T., Abe, I.		Authors: Tao, H., Mori, T., Abe, I.

OCA: Protected "7eyu" [edit=sysop:move=sysop]

OCA — Wed, 09 Jun 2021 10:59:39 GMT

Protected "7eyu" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:59, 9 June 2021

OCA: New page: '''Unreleased structure''' The entry 7eyu is ON HOLD Authors: Tao, H., Mori, T., Abe, I. Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin ...

OCA — Wed, 09 Jun 2021 10:59:38 GMT

New page: '''Unreleased structure''' The entry 7eyu is ON HOLD Authors: Tao, H., Mori, T., Abe, I. Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin ...

New page

'''Unreleased structure'''

The entry 7eyu is ON HOLD

Authors: Tao, H., Mori, T., Abe, I.

Description: Fe(II)/(alpha)ketoglutarate-dependent dioxygenase SptF-N65T mutant with andiconin D
[[Category: Unreleased Structures]]
[[Category: Abe, I]]
[[Category: Mori, T]]
[[Category: Tao, H]]