7ezw - Revision history

OCA at 17:06, 29 November 2023

OCA — Wed, 29 Nov 2023 17:06:58 GMT

←Older revision		Revision as of 17:06, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ezw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EZW FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ezw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EZW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ezw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ezw OCA], [https://pdbe.org/7ezw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ezw RCSB], [https://www.ebi.ac.uk/pdbsum/7ezw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ezw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ezw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ezw OCA], [https://pdbe.org/7ezw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ezw RCSB], [https://www.ebi.ac.uk/pdbsum/7ezw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ezw ProSAT]</span></td></tr>
	</table>		</table>

OCA at 07:42, 9 November 2022

OCA — Wed, 09 Nov 2022 07:42:34 GMT

←Older revision		Revision as of 07:42, 9 November 2022
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	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>	+	[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 03:47, 8 September 2022

OCA — Thu, 08 Sep 2022 03:47:08 GMT

←Older revision		Revision as of 03:47, 8 September 2022
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-	====	+	==Cyclic Peptide that Interacts with the eIF4E Capped-mRNA Binding Site==
-	<StructureSection load='7ezw' size='340' side='right'caption='[[7ezw]]' scene=''>	+	<StructureSection load='7ezw' size='340' side='right'caption='[[7ezw]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ezw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_M13 Escherichia virus M13] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EZW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EZW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ezw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ezw OCA], [https://pdbe.org/7ezw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ezw RCSB], [https://www.ebi.ac.uk/pdbsum/7ezw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ezw ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ezw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ezw OCA], [https://pdbe.org/7ezw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ezw RCSB], [https://www.ebi.ac.uk/pdbsum/7ezw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ezw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/IF4E_HUMAN IF4E_HUMAN]] Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:16271312</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Identifying new binding sites and poses that modify biological function are an important step towards drug discovery. We have identified a novel disulphide constrained peptide that interacts with the cap-binding site of eIF4E, an attractive therapeutic target that is commonly overexpressed in many cancers and plays a significant role in initiating a cancer specific protein synthesis program though binding the 5'cap (7'methyl-guanoisine) moiety found on mammalian mRNAs. The use of disulphide constrained peptides to explore intracellular biological targets is limited by their lack of cell permeability and the instability of the disulphide bond in the reducing environment of the cell, loss of which results in abrogation of binding. To overcome these challenges, the cap-binding site interaction motif was placed in a hypervariable loop on an VH domain, and then selections performed to select a molecule that could recapitulate the interaction of the peptide with the target of interest in a process termed Peptide Epitope Linker Evolution (PELE). A novel VH domain was identified that interacted with the eIF4E cap binding site with a nanomolar affinity and that could be intracellularly expressed in mammalian cells. Additionally, it was demonstrated to specifically modulate eIF4E function by decreasing cap-dependent translation and cyclin D1 expression, common effects of eIF4F complex disruption.
		+
		+	Development of a novel peptide aptamer that interacts with the eIF4E capped-mRNA binding site using peptide epitope linker evolution (PELE).,Frosi Y, Ng S, Lin YC, Jiang S, Ramlan SR, Lama D, Verma CS, Asial I, Brown CJ RSC Chem Biol. 2022 May 19;3(7):916-930. doi: 10.1039/d2cb00099g. eCollection, 2022 Jul 6. PMID:35866173<ref>PMID:35866173</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ezw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia virus M13]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Brown CJ]]
		+	[[Category: Frosi Y]]
		+	[[Category: Ng S]]

OCA at 05:28, 8 June 2022

OCA — Wed, 08 Jun 2022 05:28:01 GMT

←Older revision		Revision as of 05:28, 8 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ezw ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ezw' size='340' side='right'caption='[[7ezw]]' scene=''>
-	~~Authors~~: ~~Brown, C~~.J.~~, Ng, S~~.~~, Frosi, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Cyclic Peptide that Interacts with~~ the ~~eIF4E Capped-mRNA Binding Site~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ezw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ezw OCA], [https://pdbe.org/7ezw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ezw RCSB], [https://www.ebi.ac.uk/pdbsum/7ezw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ezw ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Brown~~, C.J]]	+	__TOC__
-	[[Category: ~~Frosi, Y~~]]	+	</StructureSection>
-	[[Category: ~~Ng, S~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 11:38, 29 December 2021

OCA — Wed, 29 Dec 2021 11:38:19 GMT

←Older revision		Revision as of 11:38, 29 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ezw is ON HOLD	+	The entry 7ezw is ON HOLD until Paper Publication

	Authors: Brown, C.J., Ng, S., Frosi, Y.		Authors: Brown, C.J., Ng, S., Frosi, Y.

OCA: Protected "7ezw" [edit=sysop:move=sysop]

OCA — Wed, 09 Jun 2021 11:01:43 GMT

Protected "7ezw" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:01, 9 June 2021

OCA: New page: '''Unreleased structure''' The entry 7ezw is ON HOLD Authors: Brown, C.J., Ng, S., Frosi, Y. Description: Cyclic Peptide that Interacts with the eIF4E Capped-mRNA Binding Site [[Catego...

OCA — Wed, 09 Jun 2021 11:01:42 GMT

New page: '''Unreleased structure''' The entry 7ezw is ON HOLD Authors: Brown, C.J., Ng, S., Frosi, Y. Description: Cyclic Peptide that Interacts with the eIF4E Capped-mRNA Binding Site [[Catego...

New page

'''Unreleased structure'''

The entry 7ezw is ON HOLD

Authors: Brown, C.J., Ng, S., Frosi, Y.

Description: Cyclic Peptide that Interacts with the eIF4E Capped-mRNA Binding Site
[[Category: Unreleased Structures]]
[[Category: Brown, C.J]]
[[Category: Frosi, Y]]
[[Category: Ng, S]]