7f25 - Revision history

OCA at 17:08, 29 November 2023

OCA — Wed, 29 Nov 2023 17:08:12 GMT

←Older revision		Revision as of 17:08, 29 November 2023
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	==Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.==		==Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.==
-	<StructureSection load='7f25' size='340' side='right'caption='[[7f25]]' scene=''>	+	<StructureSection load='7f25' size='340' side='right'caption='[[7f25]], [[Resolution\|resolution]] 2.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F25 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f25]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F25 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f25 OCA], [https://pdbe.org/7f25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f25 RCSB], [https://www.ebi.ac.uk/pdbsum/7f25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f25 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.87Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f25 OCA], [https://pdbe.org/7f25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f25 RCSB], [https://www.ebi.ac.uk/pdbsum/7f25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f25 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SSB1_SALTY SSB1_SALTY] Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.[HAMAP-Rule:MF_00984]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Single-stranded DNA (ssDNA)-binding proteins (SSBs) play a central role in cells by participating in DNA metabolism, including replication, repair, recombination, and replication fork restart. SSBs are essential for cell survival and thus an attractive target for potential anti-pathogen chemotherapy. In this study, we determined the crystal structure and examined the size of the ssDNA-binding site of an SSB from Salmonella enterica serovar Typhimurium LT2 (SeSSB), a ubiquitous opportunistic pathogen which is highly resistant to antibiotics. The crystal structure was solved at a resolution of 2.8 A (PDB ID 7F25), indicating that the SeSSB monomer possesses an oligonucleotide/oligosaccharide-binding (OB) fold domain at its N-terminus and a flexible tail at its C-terminus. The core of the OB-fold in the SeSSB is made of a six-stranded beta-barrel capped by an alpha-helix. The crystal structure of the SeSSB contained two monomers per asymmetric unit, which may indicate the formation of a dimer. However, the gel-filtration chromatography analysis showed that the SeSSB forms a tetramer in solution. Through an electrophoretic mobility shift analysis, we characterized the stoichiometry of the SeSSB complexed with a series of ssDNA dA homopolymers, and the size of the ssDNA-binding site was determined to be around 22 nt. We also found the flavanonol taxifolin, also known as dihydroquercetin, capable of inhibiting the ssDNA-binding activity of the SeSSB. Thus, this result extended the SSB interactome to include taxifolin, a natural product with a wide range of promising pharmacological activities.
		+
		+	Crystal Structure of an SSB Protein from Salmonella enterica and Its Inhibition by Flavanonol Taxifolin.,Lin ES, Huang YH, Luo RH, Basharat Z, Huang CY Int J Mol Sci. 2022 Apr 15;23(8). pii: ijms23084399. doi: 10.3390/ijms23084399. PMID:35457218<ref>PMID:35457218</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f25" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Single-stranded DNA-binding protein 3D structures\|Single-stranded DNA-binding protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
	[[Category: Huang CY]]		[[Category: Huang CY]]
	[[Category: Huang YH]]		[[Category: Huang YH]]
	[[Category: Luo RH]]		[[Category: Luo RH]]

OCA at 12:46, 4 May 2022

OCA — Wed, 04 May 2022 12:46:12 GMT

←Older revision		Revision as of 12:46, 4 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7f25 ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.==
-		+	<StructureSection load='7f25' size='340' side='right'caption='[[7f25]]' scene=''>
-	~~Authors~~: ~~Luo~~, R.H., ~~Huang~~, Y.H., ~~Huang~~, C.Y.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F25 FirstGlance]. <br>
-	~~Description: Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f25 OCA], [https://pdbe.org/7f25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f25 RCSB], [https://www.ebi.ac.uk/pdbsum/7f25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f25 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Luo, R.H~~]]	+	__TOC__
-	[[Category: Huang~~, C.Y~~]]	+	</StructureSection>
-	[[Category: ~~Huang, Y.H~~]]	+	[[Category: Large Structures]]
		+	[[Category: Huang CY]]
		+	[[Category: Huang YH]]
		+	[[Category: Luo RH]]

OCA at 10:11, 13 October 2021

OCA — Wed, 13 Oct 2021 10:11:24 GMT

←Older revision		Revision as of 10:11, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7f25 is ON HOLD	+	The entry 7f25 is ON HOLD until Paper Publication

	Authors: Luo, R.H., Huang, Y.H., Huang, C.Y.		Authors: Luo, R.H., Huang, Y.H., Huang, C.Y.

OCA: Protected "7f25" [edit=sysop:move=sysop]

OCA — Thu, 17 Jun 2021 13:34:01 GMT

Protected "7f25" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:34, 17 June 2021

OCA: New page: '''Unreleased structure''' The entry 7f25 is ON HOLD Authors: Luo, R.H., Huang, Y.H., Huang, C.Y. Description: Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2...

OCA — Thu, 17 Jun 2021 13:33:59 GMT

New page: '''Unreleased structure''' The entry 7f25 is ON HOLD Authors: Luo, R.H., Huang, Y.H., Huang, C.Y. Description: Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2...

New page

'''Unreleased structure'''

The entry 7f25 is ON HOLD

Authors: Luo, R.H., Huang, Y.H., Huang, C.Y.

Description: Crystal structure of SSB from Salmonella enterica serovar Typhimurium LT2.
[[Category: Unreleased Structures]]
[[Category: Luo, R.H]]
[[Category: Huang, C.Y]]
[[Category: Huang, Y.H]]