7f3u - Revision history

OCA at 10:03, 12 January 2022

OCA — Wed, 12 Jan 2022 10:03:22 GMT

←Older revision		Revision as of 10:03, 12 January 2022
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	==Cryo-EM structure of human TMEM120A in the CoASH-free state==		==Cryo-EM structure of human TMEM120A in the CoASH-free state==
-	<StructureSection load='7f3u' size='340' side='right'caption='[[7f3u]]' scene=''>	+	<StructureSection load='7f3u' size='340' side='right'caption='[[7f3u]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F3U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f3u]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F3U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f3u OCA], [https://pdbe.org/7f3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f3u RCSB], [https://www.ebi.ac.uk/pdbsum/7f3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f3u ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TMEM120A, TACAN, TMPIT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f3u OCA], [https://pdbe.org/7f3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f3u RCSB], [https://www.ebi.ac.uk/pdbsum/7f3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f3u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/TACAN_HUMAN TACAN_HUMAN]] Ion channel involved in sensing mechanical pain. Contributes to mechanosensitive currents in nocireceptors and detecting mechanical pain stimuli (By similarity). May also be required for efficient adipogenesis (PubMed:26024229).[UniProtKB:Q8C1E7]<ref>PMID:26024229</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	TMEM120A, a member of the transmembrane protein 120 (TMEM120) family, has a pivotal function in adipocyte differentiation and metabolism, and may also contribute to sensing mechanical pain by functioning as an ion channel named TACAN. Here we report that expression of TMEM120A is not sufficient in mediating poking- or stretch-induced currents in cells and have solved cryo-electron microscopy (cryo-EM) structures of human TMEM120A (HsTMEM120A) in complex with an endogenous metabolic cofactor (coenzyme A, CoASH) and in the apo form. HsTMEM120A forms a symmetrical homodimer with each monomer containing an amino-terminal coiled-coil motif followed by a transmembrane domain with six membrane-spanning helices. Within the transmembrane domain, a CoASH molecule is hosted in a deep cavity and forms specific interactions with nearby amino acid residues. Mutation of a central tryptophan residue involved in binding CoASH dramatically reduced the binding affinity of HsTMEM120A with CoASH. HsTMEM120A exhibits distinct conformations at the states with or without CoASH bound. Our results suggest that TMEM120A may have alternative functional roles potentially involved in CoASH transport, sensing, or metabolism.
		+
		+	TMEM120A contains a specific coenzyme A-binding site and might not mediate poking- or stretch-induced channel activities in cells.,Rong Y, Jiang J, Gao Y, Guo J, Song D, Liu W, Zhang M, Zhao Y, Xiao B, Liu Z Elife. 2021 Aug 19;10. pii: 71474. doi: 10.7554/eLife.71474. PMID:34409941<ref>PMID:34409941</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f3u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao YW]]	+	[[Category: Gao, Y W]]
-	[[Category: Liu ZF]]	+	[[Category: Liu, Z F]]
-	[[Category: Rong Y]]	+	[[Category: Rong, Y]]
-	[[Category: Song DF]]	+	[[Category: Song, D F]]
-	[[Category: Zhao Y]]	+	[[Category: Zhao, Y]]
		+	[[Category: Membrane protein]]

OCA at 03:33, 2 July 2021

OCA — Fri, 02 Jul 2021 03:33:37 GMT

←Older revision		Revision as of 03:33, 2 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7f3u ~~is ON HOLD~~	+	==Cryo-EM structure of human TMEM120A in the CoASH-free state==
-		+	<StructureSection load='7f3u' size='340' side='right'caption='[[7f3u]]' scene=''>
-	~~Authors~~: ~~Rong, Y~~., ~~Gao, Y~~.W., ~~Song~~, D.F., ~~Zhao, Y~~., ~~Liu, Z~~.F.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F3U FirstGlance]. <br>
-	~~Description: Cryo-EM structure of human TMEM120A in the CoASH-free state~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f3u OCA], [https://pdbe.org/7f3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f3u RCSB], [https://www.ebi.ac.uk/pdbsum/7f3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f3u ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Song, D.F~~]]	+	__TOC__
-	[[Category: ~~Zhao, Y~~]]	+	</StructureSection>
-	[[Category: ~~Gao,~~ Y.W]]	+	[[Category: Large Structures]]
-	[[Category: ~~Liu, Z.F~~]]	+	[[Category: Gao YW]]
-	[[Category: ~~Rong,~~ Y]]	+	[[Category: Liu ZF]]
		+	[[Category: Rong Y]]
		+	[[Category: Song DF]]
		+	[[Category: Zhao Y]]

OCA: Protected "7f3u" [edit=sysop:move=sysop]

OCA — Fri, 25 Jun 2021 06:07:22 GMT

Protected "7f3u" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:07, 25 June 2021

OCA: New page: '''Unreleased structure''' The entry 7f3u is ON HOLD Authors: Rong, Y., Gao, Y.W., Song, D.F., Zhao, Y., Liu, Z.F. Description: Cryo-EM structure of human TMEM120A in the CoASH-free st...

OCA — Fri, 25 Jun 2021 06:07:21 GMT

New page: '''Unreleased structure''' The entry 7f3u is ON HOLD Authors: Rong, Y., Gao, Y.W., Song, D.F., Zhao, Y., Liu, Z.F. Description: Cryo-EM structure of human TMEM120A in the CoASH-free st...

New page

'''Unreleased structure'''

The entry 7f3u is ON HOLD

Authors: Rong, Y., Gao, Y.W., Song, D.F., Zhao, Y., Liu, Z.F.

Description: Cryo-EM structure of human TMEM120A in the CoASH-free state
[[Category: Unreleased Structures]]
[[Category: Song, D.F]]
[[Category: Zhao, Y]]
[[Category: Gao, Y.W]]
[[Category: Liu, Z.F]]
[[Category: Rong, Y]]