OCA at 17:10, 29 November 2023

OCA — Wed, 29 Nov 2023 17:10:16 GMT

←Older revision		Revision as of 17:10, 29 November 2023
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-	====	+	==Crystal structure of human MUS81-EME2 complex==
-	<StructureSection load='7f6l' size='340' side='right'caption='[[7f6l]]' scene=''>	+	<StructureSection load='7f6l' size='340' side='right'caption='[[7f6l]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f6l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6l OCA], [https://pdbe.org/7f6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6l RCSB], [https://www.ebi.ac.uk/pdbsum/7f6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6l ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6l OCA], [https://pdbe.org/7f6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6l RCSB], [https://www.ebi.ac.uk/pdbsum/7f6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MUS81_HUMAN MUS81_HUMAN] Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.<ref>PMID:11741546</ref> <ref>PMID:12374758</ref> <ref>PMID:12686547</ref> <ref>PMID:12721304</ref> <ref>PMID:14617801</ref> <ref>PMID:15805243</ref> <ref>PMID:17289582</ref> <ref>PMID:19595721</ref> <ref>PMID:19596235</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	MUS81 is an important structure-specific endonuclease responsible for the processing of stalled replication forks and recombination intermediates. In human, MUS81 functions by forming complexes with its regulatory subunits EME1 and EME2, playing distinct roles in G2/M and S phases. Although the structures of MUS81-EME1 have been intensively studied, there is no structure information available about MUS81-EME2. Here, we report the crystal structure of MUS81-EME2, which reveals an overall protein fold similar to that of MUS81-EME1 complex. Further biochemical and structural characterization shows that the MUS81-EME1 and MUS81-EME2 complexes are identical in substrate recognition and endonuclease activities in vitro, implying that the distinct cellular roles of the two complexes could arise from temporal controls in cells. Finally, an extensive structure-guided mutagenesis analysis provides implications for the molecular basis of how the MUS81-EME endonucleases recognize various DNA substrates in a structure-selective manner.
		+
		+	Crystal structure of the human MUS81-EME2 complex.,Hua Z, Fang Q, Zhang D, Luo Z, Yuan C, Lin Z Structure. 2022 May 5;30(5):743-752.e3. doi: 10.1016/j.str.2022.02.015. Epub 2022 , Mar 14. PMID:35290797<ref>PMID:35290797</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f6l" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hua ZK]]
		+	[[Category: Lin ZH]]
		+	[[Category: Yuan C]]
		+	[[Category: Zhang DP]]

OCA at 06:16, 2 March 2022

OCA — Wed, 02 Mar 2022 06:16:48 GMT

←Older revision		Revision as of 06:16, 2 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7f6l ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7f6l' size='340' side='right'caption='[[7f6l]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6l OCA], [https://pdbe.org/7f6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6l RCSB], [https://www.ebi.ac.uk/pdbsum/7f6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6l ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 05:26, 22 September 2021

OCA — Wed, 22 Sep 2021 05:26:33 GMT

←Older revision		Revision as of 05:26, 22 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7f6l is ON HOLD	+	The entry 7f6l is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7f6l" [edit=sysop:move=sysop]

OCA — Fri, 02 Jul 2021 02:26:09 GMT

Protected "7f6l" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:26, 2 July 2021

OCA: New page: '''Unreleased structure''' The entry 7f6l is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Fri, 02 Jul 2021 02:26:08 GMT

New page: '''Unreleased structure''' The entry 7f6l is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7f6l is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7f6l - Revision history

OCA at 17:10, 29 November 2023

OCA at 06:16, 2 March 2022

OCA at 05:26, 22 September 2021

OCA: Protected "7f6l" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7f6l is ON HOLD Authors: Description: Category: Unreleased Structures