7f6u - Revision history

OCA at 17:10, 29 November 2023

OCA — Wed, 29 Nov 2023 17:10:27 GMT

←Older revision		Revision as of 17:10, 29 November 2023
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	<StructureSection load='7f6u' size='340' side='right'caption='[[7f6u]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='7f6u' size='340' side='right'caption='[[7f6u]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7f6u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f6u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6U FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MI:2-(hydroxymethyl)butane-1,4-diol'>1MI</scene>, <scene name='pdbligand=BGQ:2-HYDROXY+BUTANE-1,4-DIOL'>BGQ</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MI:2-(hydroxymethyl)butane-1,4-diol'>1MI</scene>, <scene name='pdbligand=BGQ:2-HYDROXY+BUTANE-1,4-DIOL'>BGQ</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6u OCA], [https://pdbe.org/7f6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6u RCSB], [https://www.ebi.ac.uk/pdbsum/7f6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6u ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6u OCA], [https://pdbe.org/7f6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6u RCSB], [https://www.ebi.ac.uk/pdbsum/7f6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6u ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q53VZ2_THET8 Q53VZ2_THET8]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7f6u" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7f6u" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[ABC transporter 3D structures\|ABC transporter 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Kanaujia, S P~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: ~~Mandal, S K~~]]	+	[[Category: Kanaujia SP]]
-	[[Category: ~~Metal ion]]~~	+	[[Category: Mandal SK]]
-	~~[[Category: Secondary transporter]]~~	+
-	~~[[Category: Substrate-binding protein]]~~	+
-	~~[[Category: Symporter]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 07:27, 2 March 2022

OCA — Wed, 02 Mar 2022 07:27:05 GMT

←Older revision		Revision as of 07:27, 2 March 2022
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	==Crystal structure of metal-citrate-binding mutant (Y221A) protein (MctA) of ABC transporter in apo state==		==Crystal structure of metal-citrate-binding mutant (Y221A) protein (MctA) of ABC transporter in apo state==
-	<StructureSection load='7f6u' size='340' side='right'caption='[[7f6u]]' scene=''>	+	<StructureSection load='7f6u' size='340' side='right'caption='[[7f6u]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f6u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6U FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6u OCA], [https://pdbe.org/7f6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6u RCSB], [https://www.ebi.ac.uk/pdbsum/7f6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6u ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MI:2-(hydroxymethyl)butane-1,4-diol'>1MI</scene>, <scene name='pdbligand=BGQ:2-HYDROXY+BUTANE-1,4-DIOL'>BGQ</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6u OCA], [https://pdbe.org/7f6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6u RCSB], [https://www.ebi.ac.uk/pdbsum/7f6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6u ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	More than one third of proteins require metal ions to accomplish their functions, making them obligatory for the growth and survival of microorganisms in varying environmental niches. In prokaryotes, besides their involvement in various cellular and physiological processes, metal ions stimulate the uptake of citrate molecules. Citrate is a source of carbon and energy and is reported to be transported by secondary transporters. In Gram-positive bacteria, citrate molecules are transported in complex with divalent metal ions, whereas in Gram-negative bacteria they are translocated by Na(+)/citrate symporters. In this study, the presence of a novel divalent-metal-ion-complexed citrate-uptake system that belongs to the primary active ABC transporter superfamily is reported. For uptake, the metal-ion-complexed citrate molecules are sequestered by substrate-binding proteins (SBPs) and transferred to transmembrane domains for their transport. This study reports crystal structures of an Mg(2+)-citrate-binding protein (MctA) from the Gram-negative thermophilic bacterium Thermus thermophilus HB8 in both apo and holo forms in the resolution range 1.63-2.50 A. Despite binding various divalent metal ions, MctA possesses the coordination geometry to bind its physiological metal ion, Mg(2+). The results also suggest an extended subclassification of cluster D SBPs, which are known to bind and transport divalent-metal-ion-complexed citrate molecules. Comparative assessment of the open and closed conformations of the wild-type and mutant MctA proteins suggests a gating mechanism of ligand entry following an `asymmetric domain movement' of the N-terminal domain for substrate binding.
		+
		+	Structural and thermodynamic insights into a novel Mg(2+)-citrate-binding protein from the ABC transporter superfamily.,Mandal SK, Kanaujia SP Acta Crystallogr D Struct Biol. 2021 Dec 1;77(Pt 12):1516-1534. doi:, 10.1107/S2059798321010457. Epub 2021 Nov 11. PMID:34866608<ref>PMID:34866608</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f6u" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kanaujia SP]]	+	[[Category: Kanaujia, S P]]
-	[[Category: Mandal SK]]	+	[[Category: Mandal, S K]]
		+	[[Category: Metal ion]]
		+	[[Category: Secondary transporter]]
		+	[[Category: Substrate-binding protein]]
		+	[[Category: Symporter]]
		+	[[Category: Transport protein]]

OCA at 06:18, 1 December 2021

OCA — Wed, 01 Dec 2021 06:18:54 GMT

←Older revision		Revision as of 06:18, 1 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7f6u ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of metal-citrate-binding mutant (Y221A) protein (MctA) of ABC transporter in apo state==
-		+	<StructureSection load='7f6u' size='340' side='right'caption='[[7f6u]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6U FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6u OCA], [https://pdbe.org/7f6u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6u RCSB], [https://www.ebi.ac.uk/pdbsum/7f6u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6u ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kanaujia SP]]
		+	[[Category: Mandal SK]]

OCA at 08:39, 14 July 2021

OCA — Wed, 14 Jul 2021 08:39:50 GMT

←Older revision		Revision as of 08:39, 14 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7f6u is ON HOLD	+	The entry 7f6u is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7f6u" [edit=sysop:move=sysop]

OCA — Wed, 07 Jul 2021 08:40:29 GMT

Protected "7f6u" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:40, 7 July 2021

OCA: New page: '''Unreleased structure''' The entry 7f6u is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 07 Jul 2021 08:40:28 GMT

New page: '''Unreleased structure''' The entry 7f6u is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7f6u is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]