7f6w - Revision history

OCA at 17:10, 29 November 2023

OCA — Wed, 29 Nov 2023 17:10:28 GMT

←Older revision		Revision as of 17:10, 29 November 2023
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	==Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase==		==Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase==
-	<StructureSection load='7f6w' size='340' side='right'caption='[[7f6w]]' scene=''>	+	<StructureSection load='7f6w' size='340' side='right'caption='[[7f6w]], [[Resolution\|resolution]] 2.61Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7f6w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6W FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6w OCA], [https://pdbe.org/7f6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6w RCSB], [https://www.ebi.ac.uk/pdbsum/7f6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6w ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.607Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KAA:5-O-[(L-LYSYLAMINO)SULFONYL]ADENOSINE'>KAA</scene>, <scene name='pdbligand=PG6:1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE'>PG6</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6w OCA], [https://pdbe.org/7f6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6w RCSB], [https://www.ebi.ac.uk/pdbsum/7f6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6w ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SYKC_YEAST SYKC_YEAST]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The evolutionary necessity of aminoacyl-tRNA synthetases being associated into complex is unknown. Human lysyl-tRNA synthetase (LysRS) is one component of the multi-tRNA synthetase complex (MSC), which is not only critical for protein translation but also involved in multiple cellular pathways such as immune response, cell migration, etc. Here, combined with crystallography, CRISPR/Cas9-based genome editing, biochemistry, and cell biology analyses, we show that the structures of LysRSs from metazoan are more dynamic than those from single-celled organisms. Without the presence of MSC scaffold proteins, such as aminoacyl-tRNA synthetase complex-interacting multifunctional protein 2 (AIMP2), human LysRS is free from the MSC. The interaction with AIMP2 stabilizes the closed conformation of LysRS, thereby protects the essential aminoacylation activity under stressed conditions. Deleting AIMP2 from the human embryonic kidney 293 cells leads to retardation in cell growth in nutrient deficient mediums. Together, these results suggest that the evolutionary emergence of the MSC in metazoan might be to protect the aminoacyl-tRNA synthetase components from being modified or recruited for use in other cellular pathways.
		+
		+	Human lysyl-tRNA synthetase evolves a dynamic structure that can be stabilized by forming complex.,Wu S, Zheng L, Hei Z, Zhou JB, Li G, Li P, Wang J, Ali H, Zhou XL, Wang J, Fang P Cell Mol Life Sci. 2022 Feb 8;79(2):128. doi: 10.1007/s00018-022-04158-9. PMID:35133502<ref>PMID:35133502</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7f6w" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Saccharomyces cerevisiae]]
	[[Category: Fang P]]		[[Category: Fang P]]
	[[Category: Hei Z]]		[[Category: Hei Z]]

OCA at 10:15, 16 February 2022

OCA — Wed, 16 Feb 2022 10:15:27 GMT

←Older revision		Revision as of 10:15, 16 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7f6w ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase==
-		+	<StructureSection load='7f6w' size='340' side='right'caption='[[7f6w]]' scene=''>
-	~~Authors~~: ~~Wu, S~~., ~~Li, P~~., ~~Hei, Z~~., ~~Zheng, L~~., ~~Wang, J~~., ~~Fang, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F6W FirstGlance]. <br>
-	~~Description: Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f6w OCA], [https://pdbe.org/7f6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f6w RCSB], [https://www.ebi.ac.uk/pdbsum/7f6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f6w ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Zheng, L~~]]	+	__TOC__
-	[[Category: ~~Fang, P~~]]	+	</StructureSection>
-	[[Category: ~~Wu, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Hei, Z~~]]	+	[[Category: Fang P]]
-	[[Category: ~~Wang, J~~]]	+	[[Category: Hei Z]]
-	[[Category: ~~Li, P~~]]	+	[[Category: Li P]]
		+	[[Category: Wang J]]
		+	[[Category: Wu S]]
		+	[[Category: Zheng L]]

OCA at 11:57, 17 November 2021

OCA — Wed, 17 Nov 2021 11:57:25 GMT

←Older revision		Revision as of 11:57, 17 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7f6w is ON HOLD	+	The entry 7f6w is ON HOLD until Paper Publication

	Authors: Wu, S., Li, P., Hei, Z., Zheng, L., Wang, J., Fang, P.		Authors: Wu, S., Li, P., Hei, Z., Zheng, L., Wang, J., Fang, P.
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	Description: Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase		Description: Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Zheng, L]]
	[[Category: Fang, P]]		[[Category: Fang, P]]
	[[Category: Wu, S]]		[[Category: Wu, S]]
-	[[Category: Zheng, L]]
	[[Category: Hei, Z]]		[[Category: Hei, Z]]
	[[Category: Wang, J]]		[[Category: Wang, J]]
	[[Category: Li, P]]		[[Category: Li, P]]

OCA: Protected "7f6w" [edit=sysop:move=sysop]

OCA — Wed, 07 Jul 2021 08:40:34 GMT

Protected "7f6w" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:40, 7 July 2021

OCA: New page: '''Unreleased structure''' The entry 7f6w is ON HOLD Authors: Wu, S., Li, P., Hei, Z., Zheng, L., Wang, J., Fang, P. Description: Crystal structure of Saccharomyces cerevisiae lysyl-tR...

OCA — Wed, 07 Jul 2021 08:40:33 GMT

New page: '''Unreleased structure''' The entry 7f6w is ON HOLD Authors: Wu, S., Li, P., Hei, Z., Zheng, L., Wang, J., Fang, P. Description: Crystal structure of Saccharomyces cerevisiae lysyl-tR...

New page

'''Unreleased structure'''

The entry 7f6w is ON HOLD

Authors: Wu, S., Li, P., Hei, Z., Zheng, L., Wang, J., Fang, P.

Description: Crystal structure of Saccharomyces cerevisiae lysyl-tRNA Synthetase
[[Category: Unreleased Structures]]
[[Category: Fang, P]]
[[Category: Wu, S]]
[[Category: Zheng, L]]
[[Category: Hei, Z]]
[[Category: Wang, J]]
[[Category: Li, P]]