7ffg - Revision history

OCA at 17:15, 29 November 2023

OCA — Wed, 29 Nov 2023 17:15:09 GMT

←Older revision		Revision as of 17:15, 29 November 2023
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	<StructureSection load='7ffg' size='340' side='right'caption='[[7ffg]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='7ffg' size='340' side='right'caption='[[7ffg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ffg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ffg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquilaria_sinensis Aquilaria sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffg OCA], [https://pdbe.org/7ffg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffg RCSB], [https://www.ebi.ac.uk/pdbsum/7ffg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffg OCA], [https://pdbe.org/7ffg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffg RCSB], [https://www.ebi.ac.uk/pdbsum/7ffg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A385MEG6_9ROSI A0A385MEG6_9ROSI]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aquilaria sinensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kodama, T]]	+	[[Category: Kodama T]]
-	[[Category: Lee, Y]]	+	[[Category: Lee Y]]
-	[[Category: Liu, Q]]	+	[[Category: Liu Q]]
-	[[Category: Morita, H]]	+	[[Category: Morita H]]
-	[[Category: Nakashima, Y]]	+	[[Category: Nakashima Y]]
-	[[Category: Wong~~, C P]]~~	+	[[Category: Wong CP]]
-	~~[[Category: Diarylpentanoid]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Type-iii polyketide synthase~~]]	+

OCA at 10:31, 6 April 2022

OCA — Wed, 06 Apr 2022 10:31:39 GMT

←Older revision		Revision as of 10:31, 6 April 2022
Line 1:		Line 1:

	==Diarylpentanoid-producing polyketide synthase (N199F mutant)==		==Diarylpentanoid-producing polyketide synthase (N199F mutant)==
-	<StructureSection load='7ffg' size='340' side='right'caption='[[7ffg]]' scene=''>	+	<StructureSection load='7ffg' size='340' side='right'caption='[[7ffg]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ffg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffg OCA], [https://pdbe.org/7ffg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffg RCSB], [https://www.ebi.ac.uk/pdbsum/7ffg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffg ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffg OCA], [https://pdbe.org/7ffg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffg RCSB], [https://www.ebi.ac.uk/pdbsum/7ffg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffg ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	2-(2-Phenylethyl)chromones (PECs) are the principal constituents contributing to the distinctive fragrance of agarwood. How PECs are biosynthesized is currently unknown. In this work, we describe a diarylpentanoid-producing polyketide synthase (PECPS) identified from Aquilaria sinensis. Through biotransformation experiments using fluorine-labeled substrate, transient expression of PECPS in Nicotiana benthamiana, and knockdown of PECPS expression in A. sinensis calli, we demonstrate that the C6-C5-C6 scaffold of diarylpentanoid is the common precursor of PECs, and PECPS plays a crucial role in PECs biosynthesis. Crystal structure (1.98 A) analyses and site-directed mutagenesis reveal that, due to its small active site cavity (247 A(3)), PECPS employs a one-pot formation mechanism including a "diketide-CoA intermediate-released" step for the formation of the C6-C5-C6 scaffold. The identification of PECPS, the pivotal enzyme of PECs biosynthesis, provides insight into not only the feasibility of overproduction of pharmaceutically important PECs using metabolic engineering approaches, but also further exploration of how agarwood is formed.
		+
		+	Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood.,Wang XH, Gao BW, Nakashima Y, Mori T, Zhang ZX, Kodama T, Lee YE, Zhang ZK, Wong CP, Liu QQ, Qi BW, Wang J, Li J, Liu X, Abe I, Morita H, Tu PF, Shi SP Nat Commun. 2022 Jan 17;13(1):348. doi: 10.1038/s41467-022-27971-z. PMID:35039506<ref>PMID:35039506</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ffg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kodama T]]	+	[[Category: Kodama, T]]
-	[[Category: Lee Y]]	+	[[Category: Lee, Y]]
-	[[Category: Liu Q]]	+	[[Category: Liu, Q]]
-	[[Category: Morita H]]	+	[[Category: Morita, H]]
-	[[Category: Nakashima Y]]	+	[[Category: Nakashima, Y]]
-	[[Category: Wong CP]]	+	[[Category: Wong, C P]]
		+	[[Category: Diarylpentanoid]]
		+	[[Category: Transferase]]
		+	[[Category: Type-iii polyketide synthase]]

OCA at 07:27, 19 January 2022

OCA — Wed, 19 Jan 2022 07:27:58 GMT

←Older revision		Revision as of 07:27, 19 January 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ffg ~~is ON HOLD until Paper Publication~~	+	==Diarylpentanoid-producing polyketide synthase (N199F mutant)==
-		+	<StructureSection load='7ffg' size='340' side='right'caption='[[7ffg]]' scene=''>
-	~~Authors~~: ~~Morita, H~~., ~~Wong, C~~.P., ~~Liu, Q~~., ~~Kodama, T~~., ~~Lee, Y~~., ~~Nakashima, Y~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FFG FirstGlance]. <br>
-	~~Description: Diarylpentanoid-producing polyketide synthase (N199F mutant)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ffg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ffg OCA], [https://pdbe.org/7ffg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ffg RCSB], [https://www.ebi.ac.uk/pdbsum/7ffg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ffg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Liu, Q~~]]	+	__TOC__
-	[[Category: ~~Nakashima,~~ Y]]	+	</StructureSection>
-	[[Category: ~~Morita, H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lee, Y~~]]	+	[[Category: Kodama T]]
-	[[Category: ~~Kodama, T~~]]	+	[[Category: Lee Y]]
-	[[Category: Wong~~, C.P~~]]	+	[[Category: Liu Q]]
		+	[[Category: Morita H]]
		+	[[Category: Nakashima Y]]
		+	[[Category: Wong CP]]

OCA at 04:55, 18 August 2021

OCA — Wed, 18 Aug 2021 04:55:24 GMT

←Older revision		Revision as of 04:55, 18 August 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ffg is ON HOLD	+	The entry 7ffg is ON HOLD until Paper Publication

	Authors: Morita, H., Wong, C.P., Liu, Q., Kodama, T., Lee, Y., Nakashima, Y.		Authors: Morita, H., Wong, C.P., Liu, Q., Kodama, T., Lee, Y., Nakashima, Y.
Line 7:		Line 7:
	Description: Diarylpentanoid-producing polyketide synthase (N199F mutant)		Description: Diarylpentanoid-producing polyketide synthase (N199F mutant)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Nakashima, Y]]
-	[[Category: Kodama, T]]
	[[Category: Liu, Q]]		[[Category: Liu, Q]]
-	[[Category: ~~Wong~~, ~~C.P~~]]	+	[[Category: Nakashima, Y]]
	[[Category: Morita, H]]		[[Category: Morita, H]]
	[[Category: Lee, Y]]		[[Category: Lee, Y]]
		+	[[Category: Kodama, T]]
		+	[[Category: Wong, C.P]]

OCA: Protected "7ffg" [edit=sysop:move=sysop]

OCA — Wed, 28 Jul 2021 09:22:49 GMT

Protected "7ffg" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:22, 28 July 2021

OCA: New page: '''Unreleased structure''' The entry 7ffg is ON HOLD Authors: Morita, H., Wong, C.P., Liu, Q., Kodama, T., Lee, Y., Nakashima, Y. Description: Diarylpentanoid-producing polyketide synt...

OCA — Wed, 28 Jul 2021 09:22:48 GMT

New page: '''Unreleased structure''' The entry 7ffg is ON HOLD Authors: Morita, H., Wong, C.P., Liu, Q., Kodama, T., Lee, Y., Nakashima, Y. Description: Diarylpentanoid-producing polyketide synt...

New page

'''Unreleased structure'''

The entry 7ffg is ON HOLD

Authors: Morita, H., Wong, C.P., Liu, Q., Kodama, T., Lee, Y., Nakashima, Y.

Description: Diarylpentanoid-producing polyketide synthase (N199F mutant)
[[Category: Unreleased Structures]]
[[Category: Nakashima, Y]]
[[Category: Kodama, T]]
[[Category: Liu, Q]]
[[Category: Wong, C.P]]
[[Category: Morita, H]]
[[Category: Lee, Y]]