7fg6 - Revision history

OCA at 17:15, 29 November 2023

OCA — Wed, 29 Nov 2023 17:15:27 GMT

←Older revision		Revision as of 17:15, 29 November 2023
Line 1:		Line 1:

	==Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in Nanoarchaeum equitans==		==Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in Nanoarchaeum equitans==
-	<StructureSection load='7fg6' size='340' side='right'caption='[[7fg6]]' scene=''>	+	<StructureSection load='7fg6' size='340' side='right'caption='[[7fg6]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FG6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7fg6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FG6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fg6 OCA], [https://pdbe.org/7fg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fg6 RCSB], [https://www.ebi.ac.uk/pdbsum/7fg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fg6 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fg6 OCA], [https://pdbe.org/7fg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fg6 RCSB], [https://www.ebi.ac.uk/pdbsum/7fg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fg6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SYY_NANEQ SYY_NANEQ] Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).[HAMAP-Rule:MF_02009]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	tRNA(Tyr) of Nanoarchaeum equitans has a remarkable feature with an extra guanosine residue at the 5'-terminus. However, the N. equitans tRNA(Tyr) mutant without extra guanosine at the 5'-end was tyrosylated by tyrosyl-tRNA synthase (TyrRS). We solved the crystal structure of N. equitans TyrRS at 2.80 A resolution. By comparing the present solved structure with the complex structures TyrRS with tRNA(Tyr) of Thermus thermophilus and Methanocaldococcus jannaschii, an arginine substitution mutant of N. equitans TyrRS at Ile200 (I200R), which is the putative closest candidate to the 5'-phosphate of C1 of N. equitans tRNA(Tyr), was prepared. The I200R mutant tyrosylated not only wild-type tRNA(Tyr) but also the tRNA without the G-1 residue. Further tyrosylation analysis revealed that the second base of the anticodon (U35), discriminator base (A73), and C1:G72 base pair are strong recognition sites.
		+
		+	Crystal structure of Nanoarchaeum equitans tyrosyl-tRNA synthetase and its aminoacylation activity toward tRNA(Tyr) with an extra guanosine residue at the 5'-terminus.,Horikoshi T, Noguchi H, Umehara T, Mutsuro-Aoki H, Kurihara R, Noguchi R, Hashimoto T, Watanabe Y, Ando T, Kamata K, Park SY, Tamura K Biochem Biophys Res Commun. 2021 Aug 27;575:90-95. doi:, 10.1016/j.bbrc.2021.08.070. PMID:34461441<ref>PMID:34461441</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7fg6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aminoacyl tRNA synthetase 3D structures\|Aminoacyl tRNA synthetase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Nanoarchaeum equitans Kin4-M]]
	[[Category: Kamata K]]		[[Category: Kamata K]]
	[[Category: Noguchi H]]		[[Category: Noguchi H]]
	[[Category: Park SY]]		[[Category: Park SY]]
	[[Category: Tamura K]]		[[Category: Tamura K]]

OCA at 08:57, 8 September 2021

OCA — Wed, 08 Sep 2021 08:57:06 GMT

←Older revision		Revision as of 08:57, 8 September 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7fg6 ~~is ON HOLD~~	+	==Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in Nanoarchaeum equitans==
-		+	<StructureSection load='7fg6' size='340' side='right'caption='[[7fg6]]' scene=''>
-	~~Authors~~: ~~Noguchi~~, H., ~~Kamata, K~~., ~~Park, S~~.Y., ~~Tamura~~, K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FG6 FirstGlance]. <br>
-	~~Description: Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in Nanoarchaeum equitans~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fg6 OCA], [https://pdbe.org/7fg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fg6 RCSB], [https://www.ebi.ac.uk/pdbsum/7fg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fg6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Noguchi, H~~]]	+	__TOC__
-	[[Category: ~~Park, S.Y~~]]	+	</StructureSection>
-	[[Category: ~~Kamata, K~~]]	+	[[Category: Large Structures]]
-	[[Category: Tamura, K]]	+	[[Category: Kamata K]]
		+	[[Category: Noguchi H]]
		+	[[Category: Park SY]]
		+	[[Category: Tamura K]]

OCA: Protected "7fg6" [edit=sysop:move=sysop]

OCA — Wed, 04 Aug 2021 09:05:23 GMT

Protected "7fg6" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:05, 4 August 2021

OCA: New page: '''Unreleased structure''' The entry 7fg6 is ON HOLD Authors: Noguchi, H., Kamata, K., Park, S.Y., Tamura, K. Description: Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in N...

OCA — Wed, 04 Aug 2021 09:05:23 GMT

New page: '''Unreleased structure''' The entry 7fg6 is ON HOLD Authors: Noguchi, H., Kamata, K., Park, S.Y., Tamura, K. Description: Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in N...

New page

'''Unreleased structure'''

The entry 7fg6 is ON HOLD

Authors: Noguchi, H., Kamata, K., Park, S.Y., Tamura, K.

Description: Crystal structure of the Tyrosyl-tRNA synthetase (TyrRS) in Nanoarchaeum equitans
[[Category: Unreleased Structures]]
[[Category: Noguchi, H]]
[[Category: Park, S.Y]]
[[Category: Kamata, K]]
[[Category: Tamura, K]]