7fha - Revision history

OCA at 17:15, 29 November 2023

OCA — Wed, 29 Nov 2023 17:15:56 GMT

←Older revision		Revision as of 17:15, 29 November 2023
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	<StructureSection load='7fha' size='340' side='right'caption='[[7fha]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='7fha' size='340' side='right'caption='[[7fha]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7fha]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FHA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7fha]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FHA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fha OCA], [https://pdbe.org/7fha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fha RCSB], [https://www.ebi.ac.uk/pdbsum/7fha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fha ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fha OCA], [https://pdbe.org/7fha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fha RCSB], [https://www.ebi.ac.uk/pdbsum/7fha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fha ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/PAPS2_HUMAN PAPS2_HUMAN]] Defects in PAPSS2 are the cause of spondyloepimetaphyseal dysplasia Pakistani type (SEMD-PA) [MIM:[https://omim.org/entry/612847 612847]]. A bone disease characterized by epiphyseal dysplasia with mild metaphyseal abnormalities. Clinical features include short stature evidenced at birth, short and bowed lower limbs, mild brachydactyly, kyphoscoliosis, abnormal gait, enlarged knee joints. Some patients may manifest premature pubarche and hyperandrogenism associated with skeletal dysplasia and short stature.<ref>PMID:19474428</ref> <ref>PMID:9714015</ref>	+	[https://www.uniprot.org/uniprot/PAPS2_HUMAN PAPS2_HUMAN] Defects in PAPSS2 are the cause of spondyloepimetaphyseal dysplasia Pakistani type (SEMD-PA) [MIM:[https://omim.org/entry/612847 612847]. A bone disease characterized by epiphyseal dysplasia with mild metaphyseal abnormalities. Clinical features include short stature evidenced at birth, short and bowed lower limbs, mild brachydactyly, kyphoscoliosis, abnormal gait, enlarged knee joints. Some patients may manifest premature pubarche and hyperandrogenism associated with skeletal dysplasia and short stature.<ref>PMID:19474428</ref> <ref>PMID:9714015</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PAPS2_HUMAN PAPS2_HUMAN]] Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity).	+	[https://www.uniprot.org/uniprot/PAPS2_HUMAN PAPS2_HUMAN] Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Zhang, L]]	+	[[Category: Zhang L]]
-	[[Category: Zhang, P]]	+	[[Category: Zhang P]]
-	~~[[Category: Atp sulfurylase]]~~	+
-	~~[[Category: Biosynthetic protein~~]]	+

OCA at 11:01, 16 February 2022

OCA — Wed, 16 Feb 2022 11:01:05 GMT

←Older revision		Revision as of 11:01, 16 February 2022
Line 1:		Line 1:

	==Crystal structure of the ATP sulfurylase domain of human PAPSS2 in complex with APS==		==Crystal structure of the ATP sulfurylase domain of human PAPSS2 in complex with APS==
-	<StructureSection load='7fha' size='340' side='right'caption='[[7fha]]' scene=''>	+	<StructureSection load='7fha' size='340' side='right'caption='[[7fha]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FHA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7fha]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FHA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fha OCA], [https://pdbe.org/7fha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fha RCSB], [https://www.ebi.ac.uk/pdbsum/7fha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fha ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADX:ADENOSINE-5-PHOSPHOSULFATE'>ADX</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fha OCA], [https://pdbe.org/7fha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fha RCSB], [https://www.ebi.ac.uk/pdbsum/7fha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fha ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/PAPS2_HUMAN PAPS2_HUMAN]] Defects in PAPSS2 are the cause of spondyloepimetaphyseal dysplasia Pakistani type (SEMD-PA) [MIM:[https://omim.org/entry/612847 612847]]. A bone disease characterized by epiphyseal dysplasia with mild metaphyseal abnormalities. Clinical features include short stature evidenced at birth, short and bowed lower limbs, mild brachydactyly, kyphoscoliosis, abnormal gait, enlarged knee joints. Some patients may manifest premature pubarche and hyperandrogenism associated with skeletal dysplasia and short stature.<ref>PMID:19474428</ref> <ref>PMID:9714015</ref>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/PAPS2_HUMAN PAPS2_HUMAN]] Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sulfation is an essential modification on biomolecules in living cells, and 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) is its unique and universal sulfate donor. Human PAPS synthases (PAPSS1 and 2) are the only enzymes that catalyze PAPS production from inorganic sulfate. Unexpectedly, PAPSS1 and PAPSS2 do not functional complement with each other, and abnormal function of PAPSS2 but not PAPSS1 leads to numerous human diseases including bone development diseases, hormone disorder and cancers. Here, we reported the crystal structures of ATP-sulfurylase domain of human PAPSS2 (ATPS2) and ATPS2 in complex with is product 5'-phosphosulfate (APS). We demonstrated that ATPS2 recognizes the substrates by using family conserved residues located on the HXXH and PP motifs, and achieves substrate binding and releasing by employing a non-conserved phenylalanine (Phe550) through a never observed flipping mechanism. Our discovery provides additional information to better understand the biological function of PAPSS2 especially in tumorigenesis, and may facilitate the drug discovery against this enzyme.
		+
		+	Structural basis for the substrate recognition mechanism of ATP-sulfurylase domain of human PAPS synthase 2.,Zhang P, Zhang L, Hou Z, Lin H, Gao H, Zhang L Biochem Biophys Res Commun. 2022 Jan 1;586:1-7. doi: 10.1016/j.bbrc.2021.11.062. , Epub 2021 Nov 19. PMID:34818583<ref>PMID:34818583</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7fha" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Zhang L]]	+	[[Category: Zhang, L]]
-	[[Category: Zhang P]]	+	[[Category: Zhang, P]]
		+	[[Category: Atp sulfurylase]]
		+	[[Category: Biosynthetic protein]]

OCA at 06:19, 1 December 2021

OCA — Wed, 01 Dec 2021 06:19:03 GMT

←Older revision		Revision as of 06:19, 1 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7fha ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the ATP sulfurylase domain of human PAPSS2 in complex with APS==
-		+	<StructureSection load='7fha' size='340' side='right'caption='[[7fha]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FHA FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fha OCA], [https://pdbe.org/7fha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fha RCSB], [https://www.ebi.ac.uk/pdbsum/7fha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fha ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Zhang L]]
		+	[[Category: Zhang P]]

OCA at 07:23, 8 September 2021

OCA — Wed, 08 Sep 2021 07:23:06 GMT

←Older revision		Revision as of 07:23, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7fha is ON HOLD	+	The entry 7fha is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7fha" [edit=sysop:move=sysop]

OCA — Wed, 04 Aug 2021 09:07:26 GMT

Protected "7fha" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:07, 4 August 2021

OCA: New page: '''Unreleased structure''' The entry 7fha is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Aug 2021 09:07:25 GMT

New page: '''Unreleased structure''' The entry 7fha is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7fha is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]