OCA at 15:01, 18 October 2023

OCA — Wed, 18 Oct 2023 15:01:45 GMT

←Older revision		Revision as of 15:01, 18 October 2023
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-	====	+	==Crystal structure of human phosphorylated IRF-3 bound to CBP==
-	<StructureSection load='7jfl' size='340' side='right'caption='[[7jfl]]' scene=''>	+	<StructureSection load='7jfl' size='340' side='right'caption='[[7jfl]], [[Resolution\|resolution]] 1.68Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jfl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JFL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7jfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfl OCA], [~~http~~://pdbe.org/7jfl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7jfl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7jfl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.68Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfl OCA], [https://pdbe.org/7jfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jfl RCSB], [https://www.ebi.ac.uk/pdbsum/7jfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/IRF3_HUMAN IRF3_HUMAN] Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The innate immune system is the first line of defense against bacterial and viral infections. The recognition of pathogen-associated molecular patterns by the RIG-I-like receptors, TLRs, and cGAS leads to the induction of IFN-I by activating the transcription factor IRF-3. Although the mechanism of IRF-3 activation has been extensively studied, the structural basis of IRF-3 activation upon phosphorylation is not fully understood. In this study, we determined the crystal structures of phosphorylated human and mouse IRF-3 bound to CREB-binding protein (CBP), which reveal that phosphorylated IRF-3 forms a dimer via pSer(386) (pSer(379) in mouse IRF-3) and a downstream pLxIS motif. Size-exclusion chromatography and cell-based studies show that mutations of key residues interacting with pSer(386) severely impair IRF-3 activation and IFN-beta induction. By contrast, phosphorylation of Ser(396) within the pLxIS motif of human IRF-3 only plays a moderate role in IRF-3 activation. The mouse IRF-3/CBP complex structure reveals that the mechanism of mouse IRF-3 activation is similar but distinct from human IRF-3. These structural and functional studies reveal the detailed mechanism of IRF-3 activation upon phosphorylation.
		+
		+	The Structural Basis of IRF-3 Activation upon Phosphorylation.,Jing T, Zhao B, Xu P, Gao X, Chi L, Han H, Sankaran B, Li P J Immunol. 2020 Aug 21. pii: jimmunol.2000026. doi: 10.4049/jimmunol.2000026. PMID:32826280<ref>PMID:32826280</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jfl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[CREB-binding protein 3D structures\|CREB-binding protein 3D structures]]
		+	*[[Interferon regulatory factor\|Interferon regulatory factor]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Jing T]]
		+	[[Category: Li P]]
		+	[[Category: Zhao B]]

OCA at 09:37, 9 September 2020

OCA — Wed, 09 Sep 2020 09:37:42 GMT

←Older revision		Revision as of 09:37, 9 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7jfl ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7jfl' size='340' side='right'caption='[[7jfl]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7jfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfl OCA], [http://pdbe.org/7jfl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7jfl RCSB], [http://www.ebi.ac.uk/pdbsum/7jfl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:30, 26 August 2020

OCA — Wed, 26 Aug 2020 09:30:39 GMT

←Older revision		Revision as of 09:30, 26 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7jfl is ON HOLD	+	The entry 7jfl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7jfl" [edit=sysop:move=sysop]

OCA — Wed, 29 Jul 2020 09:44:56 GMT

Protected "7jfl" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:44, 29 July 2020

OCA: New page: '''Unreleased structure''' The entry 7jfl is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Jul 2020 09:44:54 GMT

New page: '''Unreleased structure''' The entry 7jfl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jfl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7jfl - Revision history

OCA at 15:01, 18 October 2023

OCA at 09:37, 9 September 2020

OCA at 09:30, 26 August 2020

OCA: Protected "7jfl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7jfl is ON HOLD Authors: Description: Category: Unreleased Structures