7jfs - Revision history

OCA at 15:01, 18 October 2023

OCA — Wed, 18 Oct 2023 15:01:59 GMT

←Older revision		Revision as of 15:01, 18 October 2023
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	<StructureSection load='7jfs' size='340' side='right'caption='[[7jfs]], [[Resolution\|resolution]] 4.60Å' scene=''>		<StructureSection load='7jfs' size='340' side='right'caption='[[7jfs]], [[Resolution\|resolution]] 4.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7jfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Clop1 Clop1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens_ATCC_13124 Clostridium perfringens ATCC 13124]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JFS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.6Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CPF_1489 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=195103 CLOP1])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfs OCA], [https://pdbe.org/7jfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jfs RCSB], [https://www.ebi.ac.uk/pdbsum/7jfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfs OCA], [https://pdbe.org/7jfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jfs RCSB], [https://www.ebi.ac.uk/pdbsum/7jfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0H2YN38_CLOP1 A0A0H2YN38_CLOP1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clop1~~]]	+	[[Category: Clostridium perfringens ATCC 13124]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boraston~~, A B~~]]	+	[[Category: Boraston AB]]
-	[[Category: Pluvinage, B]]	+	[[Category: Pluvinage B]]
-	~~[[Category: Glycopeptidase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:18, 18 August 2021

OCA — Wed, 18 Aug 2021 06:18:23 GMT

←Older revision		Revision as of 06:18, 18 August 2021
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	==The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB==		==The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB==
-	<StructureSection load='7jfs' size='340' side='right'caption='[[7jfs]]' scene=''>	+	<StructureSection load='7jfs' size='340' side='right'caption='[[7jfs]], [[Resolution\|resolution]] 4.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clop1 Clop1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JFS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfs OCA], [https://pdbe.org/7jfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jfs RCSB], [https://www.ebi.ac.uk/pdbsum/7jfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfs ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPF_1489 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=195103 CLOP1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfs OCA], [https://pdbe.org/7jfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jfs RCSB], [https://www.ebi.ac.uk/pdbsum/7jfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfs ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A challenge faced by peptidases is the recognition of highly diverse substrates. A feature of some peptidase families is the capacity to specifically use post-translationally added glycans present on their protein substrates as a recognition determinant. This is ultimately critical to enabling peptide bond hydrolysis. This class of enzyme is also frequently large and architecturally sophisticated. However, the molecular details underpinning glycan recognition by these O-glycopeptidases, the importance of these interactions, and the functional roles of their ancillary domains remain unclear. Here, using the Clostridium perfringens ZmpA, ZmpB, and ZmpC M60 peptidases as model proteins, we provide structural and functional insight into how these intricate proteins recognize glycans as part of catalytic and noncatalytic substrate recognition. Structural, kinetic, and mutagenic analyses support the key role of glycan recognition within the M60 domain catalytic site, though they point to ZmpA as an apparently inactive enzyme. Wider examination of the Zmp domain content reveals noncatalytic carbohydrate binding as a feature of these proteins. The complete three-dimensional structure of ZmpB provides rare insight into the overall molecular organization of a highly multimodular enzyme and reveals how the interplay of individual domain function may influence biological activity. O-glycopeptidases frequently occur in host-adapted microbes that inhabit or attack mucus layers. Therefore, we anticipate that these results will be fundamental to informing more detailed models of how the glycoproteins that are abundant in mucus are destroyed as part of pathogenic processes or liberated as energy sources during normal commensal lifestyles.
		+
		+	Architecturally complex O-glycopeptidases are customized for mucin recognition and hydrolysis.,Pluvinage B, Ficko-Blean E, Noach I, Stuart C, Thompson N, McClure H, Buenbrazo N, Wakarchuk W, Boraston AB Proc Natl Acad Sci U S A. 2021 Mar 9;118(10). pii: 2019220118. doi:, 10.1073/pnas.2019220118. PMID:33658366<ref>PMID:33658366</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jfs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Clop1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boraston AB]]	+	[[Category: Boraston, A B]]
-	[[Category: Pluvinage B]]	+	[[Category: Pluvinage, B]]
		+	[[Category: Glycopeptidase]]
		+	[[Category: Hydrolase]]

OCA at 11:11, 3 February 2021

OCA — Wed, 03 Feb 2021 11:11:16 GMT

←Older revision		Revision as of 11:11, 3 February 2021
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-	'''Unreleased structure'''

-	~~The entry 7jfs is ON HOLD until Paper Publication~~	+	==The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB==
-		+	<StructureSection load='7jfs' size='340' side='right'caption='[[7jfs]]' scene=''>
-	~~Authors: Pluvinage, B., Boraston, A.B.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JFS FirstGlance]. <br>
-	~~Description:~~ The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jfs OCA], [https://pdbe.org/7jfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jfs RCSB], [https://www.ebi.ac.uk/pdbsum/7jfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jfs ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Boraston~~, A.B~~]]	+	__TOC__
-	[[Category: Pluvinage, B]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Boraston AB]]
		+	[[Category: Pluvinage B]]

OCA at 04:12, 5 August 2020

OCA — Wed, 05 Aug 2020 04:12:38 GMT

←Older revision		Revision as of 04:12, 5 August 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7jfs is ON HOLD	+	The entry 7jfs is ON HOLD until Paper Publication

	Authors: Pluvinage, B., Boraston, A.B.		Authors: Pluvinage, B., Boraston, A.B.

OCA: Protected "7jfs" [edit=sysop:move=sysop]

OCA — Wed, 29 Jul 2020 09:45:19 GMT

Protected "7jfs" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:45, 29 July 2020

OCA: New page: '''Unreleased structure''' The entry 7jfs is ON HOLD Authors: Pluvinage, B., Boraston, A.B. Description: The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridi...

OCA — Wed, 29 Jul 2020 09:45:17 GMT

New page: '''Unreleased structure''' The entry 7jfs is ON HOLD Authors: Pluvinage, B., Boraston, A.B. Description: The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridi...

New page

'''Unreleased structure'''

The entry 7jfs is ON HOLD

Authors: Pluvinage, B., Boraston, A.B.

Description: The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB
[[Category: Unreleased Structures]]
[[Category: Boraston, A.B]]
[[Category: Pluvinage, B]]