OCA at 05:53, 6 October 2021

OCA — Wed, 06 Oct 2021 05:53:13 GMT

←Older revision		Revision as of 05:53, 6 October 2021
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	==Helical filaments of plant light-dependent protochlorophyllide oxidoreductase (LPOR) bound to NADPH, Pchlide, and membrane==		==Helical filaments of plant light-dependent protochlorophyllide oxidoreductase (LPOR) bound to NADPH, Pchlide, and membrane==
-	<StructureSection load='7jk9' size='340' side='right'caption='[[7jk9]]' scene=''>	+	<StructureSection load='7jk9' size='340' side='right'caption='[[7jk9]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JK9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jk9]] is a 40 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JK9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jk9 OCA], [https://pdbe.org/7jk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7jk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jk9 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=PMR:PROTOCHLOROPHYLLIDE'>PMR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PORB, At4g27440, F27G19.40 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Protochlorophyllide_reductase Protochlorophyllide reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.33 1.3.1.33] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jk9 OCA], [https://pdbe.org/7jk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7jk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jk9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/PORB_ARATH PORB_ARATH]] Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).<ref>PMID:11785941</ref> <ref>PMID:12848821</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chlorophyll biosynthesis, crucial to life on Earth, is tightly regulated because its precursors are phototoxic(1). In flowering plants, the enzyme light-dependent protochlorophyllide oxidoreductase (LPOR) captures photons to catalyse the penultimate reaction: the reduction of a double bond within protochlorophyllide (Pchlide) to generate chlorophyllide (Chlide)(2,3). In darkness, LPOR oligomerizes to facilitate photon energy transfer and catalysis(4,5). However, the complete three-dimensional structure of LPOR, the higher-order architecture of LPOR oligomers and the implications of these self-assembled states for catalysis, including how LPOR positions Pchlide and the co-factor NADPH, remain unknown. Here, we report the atomic structure of LPOR assemblies by electron cryo-microscopy. LPOR polymerizes with its substrates into helical filaments around constricted lipid bilayer tubes. Portions of LPOR and Pchlide insert into the outer membrane leaflet, targeting the product, Chlide, to the membrane for the final reaction site of chlorophyll biosynthesis. In addition to its crucial photocatalytic role, we show that in darkness LPOR filaments directly shape membranes into high-curvature tubules with the spectral properties of the prolamellar body, whose light-triggered disassembly provides lipids for thylakoid assembly. Moreover, our structure of the catalytic site challenges previously proposed reaction mechanisms(6). Together, our results reveal a new and unexpected synergy between photosynthetic membrane biogenesis and chlorophyll synthesis in plants, orchestrated by LPOR.
		+
		+	Photocatalytic LPOR forms helical lattices that shape membranes for chlorophyll synthesis.,Nguyen HC, Melo AA, Kruk J, Frost A, Gabruk M Nat Plants. 2021 Apr;7(4):437-444. doi: 10.1038/s41477-021-00885-2. Epub 2021 Apr, 19. PMID:33875834<ref>PMID:33875834</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jk9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Frost A]]	+	[[Category: Protochlorophyllide reductase]]
-	[[Category: Gabruk M]]	+	[[Category: Frost, A]]
-	[[Category: Nguyen HC]]	+	[[Category: Gabruk, M]]
		+	[[Category: Nguyen, H C]]
		+	[[Category: Ligand-protein complex]]
		+	[[Category: Light-activated]]
		+	[[Category: Photosynthesis]]
		+	[[Category: Reductase]]

OCA at 06:47, 17 March 2021

OCA — Wed, 17 Mar 2021 06:47:37 GMT

←Older revision		Revision as of 06:47, 17 March 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7jk9 is ~~ON HOLD until Paper Publication~~	+	==Helical filaments of plant light-dependent protochlorophyllide oxidoreductase (LPOR) bound to NADPH, Pchlide, and membrane==
-		+	<StructureSection load='7jk9' size='340' side='right'caption='[[7jk9]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JK9 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jk9 OCA], [https://pdbe.org/7jk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7jk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jk9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Frost A]]
		+	[[Category: Gabruk M]]
		+	[[Category: Nguyen HC]]

OCA: Protected "7jk9" [edit=sysop:move=sysop]

OCA — Wed, 05 Aug 2020 04:16:50 GMT

Protected "7jk9" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:16, 5 August 2020

OCA: New page: '''Unreleased structure''' The entry 7jk9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 05 Aug 2020 04:16:49 GMT

New page: '''Unreleased structure''' The entry 7jk9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jk9 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7jk9 - Revision history

OCA at 05:53, 6 October 2021

OCA at 06:47, 17 March 2021

OCA: Protected "7jk9" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7jk9 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures