7jt8 - Revision history

OCA at 09:17, 25 October 2023

OCA — Wed, 25 Oct 2023 09:17:17 GMT

←Older revision		Revision as of 09:17, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7jt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermus_fervidus_DSM_2088 Methanothermus fervidus DSM 2088]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JT8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7jt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermus_fervidus_DSM_2088 Methanothermus fervidus DSM 2088]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JT8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.84Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jt8 OCA], [https://pdbe.org/7jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jt8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jt8 OCA], [https://pdbe.org/7jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jt8 ProSAT]</span></td></tr>
	</table>		</table>

OCA at 12:52, 22 February 2023

OCA — Wed, 22 Feb 2023 12:52:30 GMT

←Older revision		Revision as of 12:52, 22 February 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7jt8 is ~~ON HOLD until Paper~~ Publication	+	==Apo structure of a pseudomurein peptide ligase type E from Methanothermus fervidus==
		+	<StructureSection load='7jt8' size='340' side='right'caption='[[7jt8]], [[Resolution\|resolution]] 1.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7jt8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermus_fervidus_DSM_2088 Methanothermus fervidus DSM 2088]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JT8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jt8 OCA], [https://pdbe.org/7jt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jt8 RCSB], [https://www.ebi.ac.uk/pdbsum/7jt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jt8 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/E3GZ29_METFV E3GZ29_METFV]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Archaea have diverse cell wall types, yet none are identical to bacterial peptidoglycan (murein). Methanogens Methanobacteria and Methanopyrus possess cell walls of pseudomurein, a structural analogue of murein. Pseudomurein differs from murein in containing the unique archaeal sugar N-acetyltalosaminuronic acid instead of N-acetylmuramic acid, beta-1,3 glycosidic bonds in place of beta-1,4 bonds and only l-amino acids in the peptide cross-links. We have determined crystal structures of methanogen pseudomurein peptide ligases (termed pMurE) from Methanothermus fervidus (Mfer762) and Methanothermobacter thermautotrophicus (Mth734) that are structurally most closely related to bacterial MurE peptide ligases. The homology of the archaeal pMurE and bacterial MurE enzymes is clear both in the overall structure and at the level of each of the three domains. In addition, we identified two UDP-binding sites in Mfer762 pMurE, one at the exterior surface of the interface of the N-terminal and middle domains, and a second site at an inner surface continuous with the highly conserved interface of the three domains. Residues involved in ATP binding in MurE are conserved in pMurE, suggesting that a similar ATP-binding pocket is present at the interface of the middle and the C-terminal domains of pMurE. The presence of pMurE ligases in members of the Methanobacteriales and Methanopyrales, that are structurally related to bacterial MurE ligases, supports the idea that the biosynthetic origins of archaeal pseudomurein and bacterial peptidoglycan cell walls are evolutionarily related.

-	~~Authors: Carbone~~, V., Schofield, ~~L.R.~~, Sutherland-Smith~~, A~~.J.~~, Ronimus, R~~.S.~~, Subedi, B~~.P.	+	Structural characterisation of methanogen pseudomurein cell wall peptide ligases homologous to bacterial MurE/F murein peptide ligases.,Subedi BP, Schofield LR, Carbone V, Wolf M, Martin WF, Ronimus RS, Sutherland-Smith AJ Microbiology (Reading). 2022 Sep;168(9). doi: 10.1099/mic.0.001235. PMID:36178458<ref>PMID:36178458</ref>

-	~~Description: Apo structure of~~ a ~~pseudomurein peptide~~ ligase ~~type E from Methanothermus fervidus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Ronimus, R.S~~]]	+	<div class="pdbe-citations 7jt8" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Schofield, L.R~~]]	+
-	[[Category: ~~Carbone, V~~]]	+	==See Also==
-	[[Category: ~~Sutherland-Smith, A.J~~]]	+	*[[Mur ligase\|Mur ligase]]
-	[[Category: Subedi~~, B.P~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Methanothermus fervidus DSM 2088]]
		+	[[Category: Carbone V]]
		+	[[Category: Ronimus RS]]
		+	[[Category: Schofield LR]]
		+	[[Category: Subedi BP]]
		+	[[Category: Sutherland-Smith AJ]]

OCA: Protected "7jt8" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2020 09:37:58 GMT

Protected "7jt8" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:37, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7jt8 is ON HOLD until Paper Publication Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P. Description: Apo...

OCA — Wed, 26 Aug 2020 09:37:56 GMT

New page: '''Unreleased structure''' The entry 7jt8 is ON HOLD until Paper Publication Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P. Description: Apo...

New page

'''Unreleased structure'''

The entry 7jt8 is ON HOLD until Paper Publication

Authors: Carbone, V., Schofield, L.R., Sutherland-Smith, A.J., Ronimus, R.S., Subedi, B.P.

Description: Apo structure of a pseudomurein peptide ligase type E from Methanothermus fervidus
[[Category: Unreleased Structures]]
[[Category: Ronimus, R.S]]
[[Category: Schofield, L.R]]
[[Category: Carbone, V]]
[[Category: Sutherland-Smith, A.J]]
[[Category: Subedi, B.P]]