OCA at 07:49, 25 June 2021

OCA — Fri, 25 Jun 2021 07:49:22 GMT

←Older revision		Revision as of 07:49, 25 June 2021
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	==Crystal structure of Salmonella enterica Typhimurium BcfH==		==Crystal structure of Salmonella enterica Typhimurium BcfH==
-	<StructureSection load='7jve' size='340' side='right'caption='[[7jve]]' scene=''>	+	<StructureSection load='7jve' size='340' side='right'caption='[[7jve]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JVE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jve]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JVE FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jve OCA], [https://pdbe.org/7jve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jve RCSB], [https://www.ebi.ac.uk/pdbsum/7jve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jve ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">B9C96_12120, CC725_14640, D7O80_18050, DOC60_06685, DSM38_01995, ECA50_10820, SAMEA4398682_04006 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jve OCA], [https://pdbe.org/7jve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jve RCSB], [https://www.ebi.ac.uk/pdbsum/7jve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jve ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aims: Thioredoxin (TRX)-fold proteins are ubiquitous in nature. This redox scaffold has evolved to enable a variety of functions, including redox regulation, protein folding, and oxidative stress defense. In bacteria, the TRX-like disulfide bond (Dsb) family mediates the oxidative folding of multiple proteins required for fitness and pathogenic potential. Conventionally, Dsb proteins have specific redox functions with monomeric and dimeric Dsbs exclusively catalyzing thiol oxidation and disulfide isomerization, respectively. This contrasts with the eukaryotic disulfide forming machinery where the modular TRX protein disulfide isomerase (PDI) mediates thiol oxidation and disulfide reshuffling. In this study, we identified and structurally and biochemically characterized a novel Dsb-like protein from Salmonella enterica termed bovine colonization factor protein H (BcfH) and defined its role in virulence. Results: In the conserved bovine colonization factor (bcf) fimbrial operon, the Dsb-like enzyme BcfH forms a trimeric structure, exceptionally uncommon among the large and evolutionary conserved TRX superfamily. This protein also displays very unusual catalytic redox centers, including an unwound alpha-helix holding the redox active site and a trans-proline instead of the conserved cis-proline active site loop. Remarkably, BcfH displays both thiol oxidase and disulfide isomerase activities contributing to Salmonella fimbrial biogenesis. Innovation and Conclusion: Typically, oligomerization of bacterial Dsb proteins modulates their redox function, with monomeric and dimeric Dsbs mediating thiol oxidation and disulfide isomerization, respectively. This study demonstrates a further structural and functional malleability in the TRX-fold protein family. BcfH trimeric architecture and unconventional catalytic sites permit multiple redox functions emulating in bacteria the eukaryotic PDI dual oxidoreductase activity.
		+
		+	Salmonella enterica BcfH Is a Trimeric Thioredoxin-Like Bifunctional Enzyme with Both Thiol Oxidase and Disulfide Isomerase Activities.,Subedi P, Paxman JJ, Wang G, Hor L, Hong Y, Verderosa AD, Whitten AE, Panjikar S, Santos-Martin CF, Martin JL, Totsika M, Heras B Antioxid Redox Signal. 2021 Apr 12. doi: 10.1089/ars.2020.8218. PMID:33607928<ref>PMID:33607928</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jve" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus typhimurium loeffler 1892]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Heras B]]	+	[[Category: Heras, B]]
-	[[Category: Hor L]]	+	[[Category: Hor, L]]
-	[[Category: Paxman JJ]]	+	[[Category: Paxman, J J]]
-	[[Category: Subedi P]]	+	[[Category: Subedi, P]]
		+	[[Category: Fimbrial operon]]
		+	[[Category: Isomerase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Virulence factor]]

OCA at 08:23, 21 April 2021

OCA — Wed, 21 Apr 2021 08:23:33 GMT

←Older revision		Revision as of 08:23, 21 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7jve is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Salmonella enterica Typhimurium BcfH==
-		+	<StructureSection load='7jve' size='340' side='right'caption='[[7jve]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JVE FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jve FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jve OCA], [https://pdbe.org/7jve PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jve RCSB], [https://www.ebi.ac.uk/pdbsum/7jve PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jve ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Heras B]]
		+	[[Category: Hor L]]
		+	[[Category: Paxman JJ]]
		+	[[Category: Subedi P]]

OCA at 07:40, 9 September 2020

OCA — Wed, 09 Sep 2020 07:40:33 GMT

←Older revision		Revision as of 07:40, 9 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7jve is ON HOLD	+	The entry 7jve is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7jve" [edit=sysop:move=sysop]

OCA — Wed, 26 Aug 2020 09:46:05 GMT

Protected "7jve" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:46, 26 August 2020

OCA: New page: '''Unreleased structure''' The entry 7jve is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 26 Aug 2020 09:46:04 GMT

New page: '''Unreleased structure''' The entry 7jve is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jve is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7jve - Revision history

OCA at 07:49, 25 June 2021

OCA at 08:23, 21 April 2021

OCA at 07:40, 9 September 2020

OCA: Protected "7jve" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7jve is ON HOLD Authors: Description: Category: Unreleased Structures