OCA at 15:12, 18 October 2023

OCA — Wed, 18 Oct 2023 15:12:24 GMT

←Older revision		Revision as of 15:12, 18 October 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of human histone deacetylase 8 (HDAC8) E66D/Y306F double mutation complexed with a tetrapeptide substrate==
-	<StructureSection load='7jvv' size='340' side='right'caption='[[7jvv]]' scene=''>	+	<StructureSection load='7jvv' size='340' side='right'caption='[[7jvv]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jvv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JVV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7jvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jvv OCA], [~~http~~://pdbe.org/7jvv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7jvv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7jvv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7jvv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.84Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MCM:7-AMINO-4-METHYL-CHROMEN-2-ONE'>MCM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jvv OCA], [https://pdbe.org/7jvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jvv RCSB], [https://www.ebi.ac.uk/pdbsum/7jvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jvv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/HDAC8_HUMAN HDAC8_HUMAN] Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility.<ref>PMID:10748112</ref> <ref>PMID:10926844</ref> <ref>PMID:10922473</ref> <ref>PMID:14701748</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cornelia de Lange Syndrome (CdLS) and associated spectrum disorders are characterized by one or more congenital anomalies including distinctive facial features, upper limb abnormalities, intellectual disability, and other symptoms. The molecular genetic basis of CdLS is linked to defects in cohesin, a protein complex that functions in sister chromatid cohesion, chromatin organization, and transcriptional regulation. Histone deacetylase 8 (HDAC8) plays an important role in cohesin function by catalyzing the deacetylation of SMC3, which is required for efficient recycling of the cohesin complex. Missense mutations in HDAC8 have been identified in children diagnosed with CdLS spectrum disorders, and here we outline structure-function relationships for four of these mutations. Specifically, we report the 1.50 A-resolution structure of the I45T HDAC8-suberoylanilide hydroxamic acid complex, the 1.84 A-resolution structure of E66D/Y306F HDAC8 complexed with a peptide assay substrate, and the 2.40 A-resolution structure of G320R HDAC8 complexed with the inhibitor M344. Additionally, we present a computationally generated model of D176G HDAC8. These structures illuminate new structure-function relationships for HDAC8 and highlight the importance of long-range interactions in the protein scaffold that can influence catalytic function.
		+
		+	Structural analysis of histone deacetylase 8 mutants associated with Cornelia de Lange Syndrome spectrum disorders.,Osko JD, Porter NJ, Decroos C, Lee MS, Watson PR, Raible SE, Krantz ID, Deardorff MA, Christianson DW J Struct Biol. 2020 Dec 11;213(1):107681. doi: 10.1016/j.jsb.2020.107681. PMID:33316326<ref>PMID:33316326</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jvv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Histone deacetylase 3D structures\|Histone deacetylase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Christianson DW]]
		+	[[Category: Decroos C]]
		+	[[Category: Lee M]]
		+	[[Category: Osko JD]]
		+	[[Category: Porter NJ]]

OCA at 12:36, 16 December 2020

OCA — Wed, 16 Dec 2020 12:36:17 GMT

←Older revision		Revision as of 12:36, 16 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7jvv ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7jvv' size='340' side='right'caption='[[7jvv]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7jvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jvv OCA], [http://pdbe.org/7jvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7jvv RCSB], [http://www.ebi.ac.uk/pdbsum/7jvv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7jvv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7jvv" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2020 05:23:02 GMT

Protected "7jvv" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:23, 2 September 2020

OCA: New page: '''Unreleased structure''' The entry 7jvv is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Sep 2020 05:23:01 GMT

New page: '''Unreleased structure''' The entry 7jvv is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jvv is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7jvv - Revision history

OCA at 15:12, 18 October 2023

OCA at 12:36, 16 December 2020

OCA: Protected "7jvv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7jvv is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures