7jx7 - Revision history

OCA at 15:13, 18 October 2023

OCA — Wed, 18 Oct 2023 15:13:29 GMT

←Older revision		Revision as of 15:13, 18 October 2023
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-	====	+	==BRD2-BD2 in complex with a diacetylated-H2A.Z peptide==
-	<StructureSection load='7jx7' size='340' side='right'caption='[[7jx7]]' scene=''>	+	<StructureSection load='7jx7' size='340' side='right'caption='[[7jx7]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jx7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JX7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JX7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jx7 OCA], [https://pdbe.org/7jx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jx7 RCSB], [https://www.ebi.ac.uk/pdbsum/7jx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jx7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jx7 OCA], [https://pdbe.org/7jx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jx7 RCSB], [https://www.ebi.ac.uk/pdbsum/7jx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jx7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/BRD2_HUMAN BRD2_HUMAN] May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.<ref>PMID:18406326</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Chemical modifications of histone tails influence genome accessibility and the transcriptional state of eukaryotic cells. Lysine acetylation is one of the most common modifications and acetyllysine-binding bromodomains (BDs) provide a means for acetyllysine marks to be translated into meaningful cellular responses. Here, we have investigated the mechanism underlying the reported association between the Bromodomain and Extra Terminal (BET) family of BD proteins and the essential histone variant H2A.Z. We use NMR spectroscopy to demonstrate a physical interaction between the N-terminal tail of H2A.Z and the BDs of BRD2, BRD3, and BRD4, and show that the interaction is dependent on lysine acetylation in H2A.Z. The BDs preferentially engage a diacetylated H2A.Z-K4acK7ac motif that is reminiscent of sequences found in other biologically important BET BD target proteins, including histones and transcription factors. A H2A.Z-K7acK11ac motif can also bind BET BDs-with a preference for the second BD of each protein. Chemical shift perturbation mapping of the interactions, together with an X-ray crystal structure of BRD2-BD1 bound to H2A.Z-K4acK7ac, shows that H2A.Z binds the canonical AcK binding pocket of the BDs. This mechanism mirrors the conserved binding mode that is unique to the BET BDs, in which two acetylation marks are read simultaneously by a single BD. Our findings provide structural corroboration of biochemical and cell biological data that link H2A.Z and BET-family proteins, suggesting that the function of H2A.Z is enacted through interactions with these chromatin readers.
		+
		+	The bromodomains of BET family proteins can recognize diacetylated histone H2A.Z.,Patel K, Solomon PD, Walshe JL, Low JKK, Mackay JP Protein Sci. 2021 Feb;30(2):464-476. doi: 10.1002/pro.4006. Epub 2020 Dec 8. PMID:33247496<ref>PMID:33247496</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jx7" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Bromodomain-containing protein 3D structures\|Bromodomain-containing protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Low JKK]]
		+	[[Category: Mackay JP]]
		+	[[Category: Patel K]]
		+	[[Category: Solomon PD]]

OCA at 18:06, 10 March 2021

OCA — Wed, 10 Mar 2021 18:06:34 GMT

←Older revision		Revision as of 18:06, 10 March 2021
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	<StructureSection load='7jx7' size='340' side='right'caption='[[7jx7]]' scene=''>		<StructureSection load='7jx7' size='340' side='right'caption='[[7jx7]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7jx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jx7 OCA], [~~http~~://pdbe.org/7jx7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7jx7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7jx7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7jx7 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jx7 OCA], [https://pdbe.org/7jx7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jx7 RCSB], [https://www.ebi.ac.uk/pdbsum/7jx7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jx7 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 06:41, 9 December 2020

OCA — Wed, 09 Dec 2020 06:41:58 GMT

←Older revision		Revision as of 06:41, 9 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 7jx7 ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7jx7' size='340' side='right'caption='[[7jx7]]' scene=''>
-	~~Authors~~: ~~Patel, K~~.~~, Low, J~~.K.K.~~, Mackay, J~~.P.~~, Solomon, P.D~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~BRD2-BD2 in complex with a diacetylated-H2A~~.~~Z peptide~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7jx7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jx7 OCA], [http://pdbe.org/7jx7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7jx7 RCSB], [http://www.ebi.ac.uk/pdbsum/7jx7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7jx7 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Solomon~~, P.D]]	+	__TOC__
-	[~~[Category~~: ~~Mackay~~, J.P]]	+	</StructureSection>
-	[[Category: ~~Low, J.K.K~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Patel, K~~]]	+	[[Category: Z-disk]]

OCA: Protected "7jx7" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2020 05:26:46 GMT

Protected "7jx7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:26, 2 September 2020

OCA: New page: '''Unreleased structure''' The entry 7jx7 is ON HOLD Authors: Patel, K., Low, J.K.K., Mackay, J.P., Solomon, P.D. Description: BRD2-BD2 in complex with a diacetylated-H2A.Z peptide [[C...

OCA — Wed, 02 Sep 2020 05:26:45 GMT

New page: '''Unreleased structure''' The entry 7jx7 is ON HOLD Authors: Patel, K., Low, J.K.K., Mackay, J.P., Solomon, P.D. Description: BRD2-BD2 in complex with a diacetylated-H2A.Z peptide [[C...

New page

'''Unreleased structure'''

The entry 7jx7 is ON HOLD

Authors: Patel, K., Low, J.K.K., Mackay, J.P., Solomon, P.D.

Description: BRD2-BD2 in complex with a diacetylated-H2A.Z peptide
[[Category: Unreleased Structures]]
[[Category: Solomon, P.D]]
[[Category: Mackay, J.P]]
[[Category: Low, J.K.K]]
[[Category: Patel, K]]