7jxs - Revision history

OCA at 15:14, 18 October 2023

OCA — Wed, 18 Oct 2023 15:14:09 GMT

←Older revision		Revision as of 15:14, 18 October 2023
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	<StructureSection load='7jxs' size='340' side='right'caption='[[7jxs]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='7jxs' size='340' side='right'caption='[[7jxs]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7jxs]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jxs]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JXS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gag ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jxs OCA], [https://pdbe.org/7jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jxs RCSB], [https://www.ebi.ac.uk/pdbsum/7jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jxs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jxs OCA], [https://pdbe.org/7jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jxs RCSB], [https://www.ebi.ac.uk/pdbsum/7jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jxs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GAG_HV1N5 GAG_HV1N5] Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu. Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7jxs" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7jxs" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Gag polyprotein 3D structures\|Gag polyprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human immunodeficiency virus 1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eastep~~, G N~~]]	+	[[Category: Eastep GN]]
-	[[Category: Ghanam~~, R H~~]]	+	[[Category: Ghanam RH]]
-	[[Category: Green~~, T J~~]]	+	[[Category: Green TJ]]
-	[[Category: Saad~~, J S]]~~	+	[[Category: Saad JS]]
-	~~[[Category: Gag]]~~	+
-	~~[[Category: Hiv-1]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 10:08, 14 July 2021

OCA — Wed, 14 Jul 2021 10:08:49 GMT

←Older revision		Revision as of 10:08, 14 July 2021
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	==Crystal Structure Human Immunodeficiency Virus-1 Matrix protein Mutant Q63R Crystal Form 2==		==Crystal Structure Human Immunodeficiency Virus-1 Matrix protein Mutant Q63R Crystal Form 2==
-	<StructureSection load='7jxs' size='340' side='right'caption='[[7jxs]]' scene=''>	+	<StructureSection load='7jxs' size='340' side='right'caption='[[7jxs]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JXS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jxs]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JXS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jxs OCA], [https://pdbe.org/7jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jxs RCSB], [https://www.ebi.ac.uk/pdbsum/7jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jxs ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=12P:DODECAETHYLENE+GLYCOL'>12P</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gag ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jxs OCA], [https://pdbe.org/7jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jxs RCSB], [https://www.ebi.ac.uk/pdbsum/7jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jxs ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During the late phase of HIV-1 infection, viral Gag polyproteins are targeted to the plasma membrane (PM) for assembly. Gag localization at the PM is a prerequisite for the incorporation of the envelope protein (Env) into budding particles. Gag assembly and Env incorporation are mediated by the N-terminal myristoylated matrix (MA) domain of Gag. Nonconservative mutations in the trimer interface of MA (A45E, T70R, and L75G) were found to impair Env incorporation and infectivity, leading to the hypothesis that MA trimerization is an obligatory step for Env incorporation. Conversely, Env incorporation can be rescued by a compensatory mutation in the MA trimer interface (Q63R). The impact of these MA mutations on the structure and trimerization properties of MA is not known. In this study, we employed NMR spectroscopy, X-ray crystallography, and sedimentation techniques to characterize the structure and trimerization properties of HIV-1 MA A45E, Q63R, T70R, and L75G mutant proteins. NMR data revealed that these point mutations did not alter the overall structure and folding of MA but caused minor structural perturbations in the trimer interface. Analytical ultracentrifugation data indicated that mutations had a minimal effect on the MA monomer-trimer equilibrium. The high-resolution X-ray structure of the unmyristoylated MA Q63R protein revealed hydrogen bonding between the side chains of Arg-63 and Ser-67, providing the first structural evidence for an additional intermolecular interaction in the trimer interface. These findings advance our knowledge of the interplay of MA trimerization and Env incorporation into HIV-1 particles.
		+
		+	Structural characterization of HIV-1 matrix mutants implicated in envelope incorporation.,Eastep GN, Ghanam RH, Green TJ, Saad JS J Biol Chem. 2021 Jan 21:100321. doi: 10.1016/j.jbc.2021.100321. PMID:33485964<ref>PMID:33485964</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jxs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Eastep GN]]	+	[[Category: Eastep, G N]]
-	[[Category: Ghanam RH]]	+	[[Category: Ghanam, R H]]
-	[[Category: Green TJ]]	+	[[Category: Green, T J]]
-	[[Category: Saad JS]]	+	[[Category: Saad, J S]]
		+	[[Category: Gag]]
		+	[[Category: Hiv-1]]
		+	[[Category: Structural protein]]

OCA at 06:47, 14 April 2021

OCA — Wed, 14 Apr 2021 06:47:32 GMT

←Older revision		Revision as of 06:47, 14 April 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7jxs is ~~ON HOLD~~	+	==Crystal Structure Human Immunodeficiency Virus-1 Matrix protein Mutant Q63R Crystal Form 2==
-		+	<StructureSection load='7jxs' size='340' side='right'caption='[[7jxs]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JXS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jxs OCA], [https://pdbe.org/7jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jxs RCSB], [https://www.ebi.ac.uk/pdbsum/7jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jxs ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Eastep GN]]
		+	[[Category: Ghanam RH]]
		+	[[Category: Green TJ]]
		+	[[Category: Saad JS]]

OCA: Protected "7jxs" [edit=sysop:move=sysop]

OCA — Wed, 02 Sep 2020 05:28:36 GMT

Protected "7jxs" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:28, 2 September 2020

OCA: New page: '''Unreleased structure''' The entry 7jxs is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 02 Sep 2020 05:28:34 GMT

New page: '''Unreleased structure''' The entry 7jxs is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jxs is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]