7jzs - Revision history

OCA at 15:15, 18 October 2023

OCA — Wed, 18 Oct 2023 15:15:21 GMT

←Older revision		Revision as of 15:15, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='7jzs' size='340' side='right'caption='[[7jzs]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='7jzs' size='340' side='right'caption='[[7jzs]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7jzs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"proteus_stuartii"_buttiaux_et_al._1954 "proteus~~ stuartii~~" buttiaux et al. 1954~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jzs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Providencia_stuartii Providencia stuartii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=COA:COENZYME+A'>COA</scene>~~, ~~<scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[6vr3\|6vr3]], [[6vr2\|6vr2]], [[6vta\|6vta]], [[6vou\|6vou]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>aac ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~588~~ "~~Proteus stuartii~~" ~~Buttiaux et al. 1954])~~<~~/td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Gentamicin_2'-N-acetyltransferase Gentamicin 2'-N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.59 2.3.1.59]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzs OCA], [https://pdbe.org/7jzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jzs RCSB], [https://www.ebi.ac.uk/pdbsum/7jzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzs OCA], [https://pdbe.org/7jzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jzs RCSB], [https://www.ebi.ac.uk/pdbsum/7jzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/AAC2_PROST AAC2_PROST]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Proteus stuartii buttiaux et al. 1954]]
-	[[Category: Gentamicin 2'-N-acetyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bassenden, A V~~]]	+	[[Category: Providencia stuartii]]
-	[[Category: ~~Berghuis, A M~~]]	+	[[Category: Bassenden AV]]
-	[[Category: ~~Park, J~~]]	+	[[Category: Berghuis AM]]
-	[[Category: ~~Acetyltransferase]]~~	+	[[Category: Park J]]
-	~~[[Category: Gnat superfamily]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:08, 14 July 2021

OCA — Wed, 14 Jul 2021 10:08:51 GMT

←Older revision		Revision as of 10:08, 14 July 2021
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	==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with CoA==		==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with CoA==
-	<StructureSection load='7jzs' size='340' side='right'caption='[[7jzs]]' scene=''>	+	<StructureSection load='7jzs' size='340' side='right'caption='[[7jzs]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7jzs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"proteus_stuartii"_buttiaux_et_al._1954 "proteus stuartii" buttiaux et al. 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzs OCA], [https://pdbe.org/7jzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jzs RCSB], [https://www.ebi.ac.uk/pdbsum/7jzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzs ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6vr3\|6vr3]], [[6vr2\|6vr2]], [[6vta\|6vta]], [[6vou\|6vou]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=588 "Proteus stuartii" Buttiaux et al. 1954])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Gentamicin_2'-N-acetyltransferase Gentamicin 2'-N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.59 2.3.1.59] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzs OCA], [https://pdbe.org/7jzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jzs RCSB], [https://www.ebi.ac.uk/pdbsum/7jzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzs ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plazomicin is currently the only next-generation aminoglycoside approved for clinical use that has the potential of evading the effects of widespread enzymatic resistance factors. However, plazomicin is still susceptible to the action of the resistance enzyme AAC(2')-Ia from Providencia stuartii. As the clinical use of plazomicin begins to increase, the spread of resistance factors will undoubtedly accelerate, rendering this aminoglycoside increasingly obsolete. Understanding resistance to plazomicin is an important step to ensure this aminoglycoside remains a viable treatment option for the foreseeable future. Here, we present three crystal structures of AAC(2')-Ia from P. stuartii, two in complex with acetylated aminoglycosides tobramycin and netilmicin, and one in complex with a non-substrate aminoglycoside, amikacin. Together, with our previously reported AAC(2')-Ia-acetylated plazomicin complex, these structures outline AAC(2')-Ia's specificity for a wide range of aminoglycosides. Additionally, our survey of AAC(2')-I homologues highlights the conservation of residues predicted to be involved in aminoglycoside binding, and identifies the presence of plasmid-encoded enzymes in environmental strains that confer resistance to the latest next-generation aminoglycoside. These results forecast the likely spread of plazomicin resistance and highlight the urgency for advancements in next-generation aminoglycoside design.
		+
		+	Structural and phylogenetic analyses of resistance to next-generation aminoglycosides conferred by AAC(2') enzymes.,Bassenden AV, Dumalo L, Park J, Blanchet J, Maiti K, Arya DP, Berghuis AM Sci Rep. 2021 Jun 2;11(1):11614. doi: 10.1038/s41598-021-89446-3. PMID:34078922<ref>PMID:34078922</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7jzs" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Proteus stuartii buttiaux et al. 1954]]
		+	[[Category: Gentamicin 2'-N-acetyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bassenden AV]]	+	[[Category: Bassenden, A V]]
-	[[Category: Berghuis AM]]	+	[[Category: Berghuis, A M]]
-	[[Category: Park J]]	+	[[Category: Park, J]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Gnat superfamily]]
		+	[[Category: Transferase]]

OCA at 14:35, 2 June 2021

OCA — Wed, 02 Jun 2021 14:35:08 GMT

←Older revision		Revision as of 14:35, 2 June 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7jzs is ~~ON HOLD until Paper Publication~~	+	==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with CoA==
-		+	<StructureSection load='7jzs' size='340' side='right'caption='[[7jzs]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7JZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7JZS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7jzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7jzs OCA], [https://pdbe.org/7jzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7jzs RCSB], [https://www.ebi.ac.uk/pdbsum/7jzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7jzs ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bassenden AV]]
		+	[[Category: Berghuis AM]]
		+	[[Category: Park J]]

OCA: Protected "7jzs" [edit=sysop:move=sysop]

OCA — Wed, 09 Sep 2020 07:46:38 GMT

Protected "7jzs" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:46, 9 September 2020

OCA: New page: '''Unreleased structure''' The entry 7jzs is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Sep 2020 07:46:37 GMT

New page: '''Unreleased structure''' The entry 7jzs is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7jzs is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]