7k2x - Revision history

OCA at 15:16, 18 October 2023

OCA — Wed, 18 Oct 2023 15:16:38 GMT

←Older revision		Revision as of 15:16, 18 October 2023
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	<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7k2x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7k2x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>blaCTX-M ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [https://pdbe.org/7k2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [https://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [https://pdbe.org/7k2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [https://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9L5C7_ECOLX Q9L5C7_ECOLX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hu, L]]	+	[[Category: Hu L]]
-	[[Category: Lu, S]]	+	[[Category: Lu S]]
-	[[Category: Palzkill, T]]	+	[[Category: Palzkill T]]
-	[[Category: Prasad~~, B V.V~~]]	+	[[Category: Prasad BVV]]
-	[[Category: Sankaran, B]]	+	[[Category: Sankaran B]]
-	[[Category: Soeung, V]]	+	[[Category: Soeung V]]
-	~~[[Category: Antibiotic resistance]]~~	+
-	~~[[Category: Ctx-m-14]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 03:49, 2 July 2021

OCA — Fri, 02 Jul 2021 03:49:21 GMT

←Older revision		Revision as of 03:49, 2 July 2021
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	==Crystal structure of CTX-M-14 E166A/K234R Beta-lactamase==		==Crystal structure of CTX-M-14 E166A/K234R Beta-lactamase==
-	<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]]' scene=''>	+	<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7k2x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [https://pdbe.org/7k2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [https://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">blaCTX-M ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [https://pdbe.org/7k2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [https://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lys(234) is one of the residues present in class A beta-lactamases that is under selective pressure due to antibiotic use. Located adjacent to proton shuttle residue Ser(130), it is suggested to play a role in proton transfer during catalysis of the antibiotics. The mechanism underpinning how substitutions in this position modulate inhibitor efficiency and substrate specificity leading to drug resistance is unclear. The K234R substitution identified in several inhibitor-resistant beta-lactamase variants is associated with decreased potency of the inhibitor clavulanic acid, which is used in combination with amoxicillin to overcome beta-lactamase-mediated antibiotic resistance. Here we show that for CTX-M-14 beta-lactamase, whereas Lys(234) is required for hydrolysis of cephalosporins such as cefotaxime, either lysine or arginine is sufficient for hydrolysis of ampicillin. Further, by determining the acylation and deacylation rates for cefotaxime hydrolysis, we show that both rates are fast, and neither is rate-limiting. The K234R substitution causes a 1500-fold decrease in the cefotaxime acylation rate but a 5-fold increase in k cat for ampicillin, suggesting that the K234R enzyme is a good penicillinase but a poor cephalosporinase due to slow acylation. Structural results suggest that the slow acylation by the K234R enzyme is due to a conformational change in Ser(130), and this change also leads to decreased inhibition potency of clavulanic acid. Because other inhibitor resistance mutations also act through changes at Ser(130) and such changes drastically reduce cephalosporin but not penicillin hydrolysis, we suggest that clavulanic acid paired with an oxyimino-cephalosporin rather than penicillin would impede the evolution of resistance.
		+
		+	A drug-resistant beta-lactamase variant changes the conformation of its active-site proton shuttle to alter substrate specificity and inhibitor potency.,Soeung V, Lu S, Hu L, Judge A, Sankaran B, Prasad BVV, Palzkill T J Biol Chem. 2020 Dec 25;295(52):18239-18255. doi: 10.1074/jbc.RA120.016103. Epub, 2020 Oct 26. PMID:33109613<ref>PMID:33109613</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7k2x" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]		*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Beta-lactamase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hu L]]	+	[[Category: Hu, L]]
-	[[Category: Lu S]]	+	[[Category: Lu, S]]
-	[[Category: Palzkill T]]	+	[[Category: Palzkill, T]]
-	[[Category: Prasad ~~BVV~~]]	+	[[Category: Prasad, B V.V]]
-	[[Category: Soeung V]]	+	[[Category: Sankaran, B]]
		+	[[Category: Soeung, V]]
		+	[[Category: Antibiotic resistance]]
		+	[[Category: Ctx-m-14]]
		+	[[Category: Hydrolase]]

OCA at 10:25, 19 May 2021

OCA — Wed, 19 May 2021 10:25:15 GMT

←Older revision		Revision as of 10:25, 19 May 2021
Line 3:		Line 3:
	<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]]' scene=''>		<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [~~http~~://pdbe.org/7k2x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [https://pdbe.org/7k2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [https://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>
	</table>		</table>
		+
		+	==See Also==
		+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:27, 4 November 2020

OCA — Wed, 04 Nov 2020 07:27:03 GMT

←Older revision		Revision as of 07:27, 4 November 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7k2x is ~~ON HOLD~~	+	==Crystal structure of CTX-M-14 E166A/K234R Beta-lactamase==
-		+	<StructureSection load='7k2x' size='340' side='right'caption='[[7k2x]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K2X OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7K2X FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7k2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k2x OCA], [http://pdbe.org/7k2x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7k2x RCSB], [http://www.ebi.ac.uk/pdbsum/7k2x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7k2x ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hu L]]
		+	[[Category: Lu S]]
		+	[[Category: Palzkill T]]
		+	[[Category: Prasad BVV]]
		+	[[Category: Soeung V]]

OCA: Protected "7k2x" [edit=sysop:move=sysop]

OCA — Wed, 16 Sep 2020 07:08:55 GMT

Protected "7k2x" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:08, 16 September 2020

OCA: New page: '''Unreleased structure''' The entry 7k2x is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Sep 2020 07:08:54 GMT

New page: '''Unreleased structure''' The entry 7k2x is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7k2x is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]