7k52 - Revision history

OCA at 19:35, 29 May 2024

OCA — Wed, 29 May 2024 19:35:20 GMT

←Older revision		Revision as of 19:35, 29 May 2024
Line 1:		Line 1:

-	====	+	==Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)==
-	<StructureSection load='7k52' size='340' side='right'caption='[[7k52]]' scene=''>	+	<StructureSection load='7k52' size='340' side='right'caption='[[7k52]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7k52]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K52 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k52 OCA], [https://pdbe.org/7k52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k52 RCSB], [https://www.ebi.ac.uk/pdbsum/7k52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k52 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRO:PROLINE'>PRO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k52 OCA], [https://pdbe.org/7k52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k52 RCSB], [https://www.ebi.ac.uk/pdbsum/7k52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k52 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RS9_ECOLI RS9_ECOLI] The C-terminal tail plays a role in the affinity of the 30S P site for different tRNAs. Mutations that decrease this affinity are suppressed in the 70S ribosome.<ref>PMID:15308780</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. How and where in the elongation cycle +1-frameshifting occurs remains poorly understood. We describe seven ~3.5-A-resolution cryo-EM structures of 70S ribosome complexes, allowing visualization of elongation and translocation by the GTPase elongation factor G (EF-G). Four structures with a + 1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G*GDPCP, the tRNA shifts to the +1-frame near the P site, rendering the freed mRNA base to bulge between the P and E sites and to stack on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.
		+
		+	Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.,Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA Nat Commun. 2021 Jul 30;12(1):4644. doi: 10.1038/s41467-021-24911-1. PMID:34330903<ref>PMID:34330903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7k52" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
		+	*[[Ribosome 3D structures\|Ribosome 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Demo G]]
		+	[[Category: Gamper HB]]
		+	[[Category: Hou YM]]
		+	[[Category: Korostelev AA]]
		+	[[Category: Loveland AB]]
		+	[[Category: Svidritskiy E]]

OCA at 10:33, 28 July 2021

OCA — Wed, 28 Jul 2021 10:33:27 GMT

←Older revision		Revision as of 10:33, 28 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7k52 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7k52' size='340' side='right'caption='[[7k52]]' scene=''>
-	~~Authors~~: ~~Demo, G~~.~~, Loveland, A~~.B.~~, Svidritskiy, E~~.~~, Gamper, H~~.B.~~, Hou, Y~~.M., ~~Korostelev, A~~.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Near post~~-~~translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k52 OCA], [https://pdbe.org/7k52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k52 RCSB], [https://www.ebi.ac.uk/pdbsum/7k52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k52 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	~~[[Category: Demo~~, ~~G]]~~	+	__TOC__
-	[~~[Category~~: ~~Hou, Y~~.M]]	+	</StructureSection>
-	[~~[Category~~: ~~Loveland, A~~.B]]	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Korostelev, A~~.A]]	+	[[Category: Z-disk]]
-	[[Category: ~~Gamper, H.B~~]]	+
-	[[Category: ~~Svidritskiy, E~~]]	+

OCA at 04:18, 14 October 2020

OCA — Wed, 14 Oct 2020 04:18:30 GMT

←Older revision		Revision as of 04:18, 14 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7k52 is ON HOLD	+	The entry 7k52 is ON HOLD until Paper Publication

	Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.		Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.

OCA: Protected "7k52" [edit=sysop:move=sysop]

OCA — Wed, 23 Sep 2020 09:15:16 GMT

Protected "7k52" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:15, 23 September 2020

OCA: New page: '''Unreleased structure''' The entry 7k52 is ON HOLD Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A. Description: Near post-translocated n...

OCA — Wed, 23 Sep 2020 09:15:15 GMT

New page: '''Unreleased structure''' The entry 7k52 is ON HOLD Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A. Description: Near post-translocated n...

New page

'''Unreleased structure'''

The entry 7k52 is ON HOLD

Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.

Description: Near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)
[[Category: Unreleased Structures]]
[[Category: Demo, G]]
[[Category: Hou, Y.M]]
[[Category: Loveland, A.B]]
[[Category: Korostelev, A.A]]
[[Category: Gamper, H.B]]
[[Category: Svidritskiy, E]]