7k54 - Revision history

OCA at 19:35, 29 May 2024

OCA — Wed, 29 May 2024 19:35:26 GMT

←Older revision		Revision as of 19:35, 29 May 2024
Line 1:		Line 1:

-	====	+	==Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS)==
-	<StructureSection load='7k54' size='340' side='right'caption='[[7k54]]' scene=''>	+	<StructureSection load='7k54' size='340' side='right'caption='[[7k54]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7k54]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K54 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k54 OCA], [https://pdbe.org/7k54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k54 RCSB], [https://www.ebi.ac.uk/pdbsum/7k54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k54 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRO:PROLINE'>PRO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k54 OCA], [https://pdbe.org/7k54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k54 RCSB], [https://www.ebi.ac.uk/pdbsum/7k54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k54 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RS7_ECOLI RS7_ECOLI] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.<ref>PMID:2461734</ref> Protein S7 is also a translational repressor protein; it regulates the expression of the str operon members to different degrees by binding to its mRNA.<ref>PMID:2461734</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Frameshifting of mRNA during translation provides a strategy to expand the coding repertoire of cells and viruses. How and where in the elongation cycle +1-frameshifting occurs remains poorly understood. We describe seven ~3.5-A-resolution cryo-EM structures of 70S ribosome complexes, allowing visualization of elongation and translocation by the GTPase elongation factor G (EF-G). Four structures with a + 1-frameshifting-prone mRNA reveal that frameshifting takes place during translocation of tRNA and mRNA. Prior to EF-G binding, the pre-translocation complex features an in-frame tRNA-mRNA pairing in the A site. In the partially translocated structure with EF-G*GDPCP, the tRNA shifts to the +1-frame near the P site, rendering the freed mRNA base to bulge between the P and E sites and to stack on the 16S rRNA nucleotide G926. The ribosome remains frameshifted in the nearly post-translocation state. Our findings demonstrate that the ribosome and EF-G cooperate to induce +1 frameshifting during tRNA-mRNA translocation.
		+
		+	Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.,Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA Nat Commun. 2021 Jul 30;12(1):4644. doi: 10.1038/s41467-021-24911-1. PMID:34330903<ref>PMID:34330903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7k54" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Demo G]]
		+	[[Category: Gamper HB]]
		+	[[Category: Hou YM]]
		+	[[Category: Korostelev AA]]
		+	[[Category: Loveland AB]]
		+	[[Category: Svidritskiy E]]

OCA at 10:33, 28 July 2021

OCA — Wed, 28 Jul 2021 10:33:30 GMT

←Older revision		Revision as of 10:33, 28 July 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7k54 ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7k54' size='340' side='right'caption='[[7k54]]' scene=''>
-	~~Authors~~: ~~Demo, G~~.~~, Loveland, A~~.B.~~, Svidritskiy, E~~.~~, Gamper, H~~.B.~~, Hou, Y~~.M., ~~Korostelev, A~~.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Mid~~-~~translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k54 OCA], [https://pdbe.org/7k54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k54 RCSB], [https://www.ebi.ac.uk/pdbsum/7k54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k54 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Korostelev, A~~.A]]	+	__TOC__
-	[~~[Category~~: ~~Gamper, H~~.B]]	+	</StructureSection>
-	~~[[Category: Svidritskiy~~, ~~E]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Demo, G]~~]	+	[[Category: Z-disk]]
-	[[Category: ~~Hou, Y.M~~]]	+
-	[[Category: ~~Loveland, A.B~~]]	+

OCA at 08:43, 7 July 2021

OCA — Wed, 07 Jul 2021 08:43:48 GMT

←Older revision		Revision as of 08:43, 7 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7k54 is ON HOLD ~~until Paper Publication~~	+	The entry 7k54 is ON HOLD

	Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.		Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.
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	Description: Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS)		Description: Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Demo, G]]
-	[[Category: Hou, Y.M]]
-	[[Category: Loveland, A.B]]
	[[Category: Korostelev, A.A]]		[[Category: Korostelev, A.A]]
	[[Category: Gamper, H.B]]		[[Category: Gamper, H.B]]
	[[Category: Svidritskiy, E]]		[[Category: Svidritskiy, E]]
		+	[[Category: Demo, G]]
		+	[[Category: Hou, Y.M]]
		+	[[Category: Loveland, A.B]]

OCA at 04:18, 14 October 2020

OCA — Wed, 14 Oct 2020 04:18:33 GMT

←Older revision		Revision as of 04:18, 14 October 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7k54 is ON HOLD	+	The entry 7k54 is ON HOLD until Paper Publication

	Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.		Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.

OCA: Protected "7k54" [edit=sysop:move=sysop]

OCA — Wed, 23 Sep 2020 09:15:22 GMT

Protected "7k54" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:15, 23 September 2020

OCA: New page: '''Unreleased structure''' The entry 7k54 is ON HOLD Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A. Description: Mid-translocated +1-fram...

OCA — Wed, 23 Sep 2020 09:15:21 GMT

New page: '''Unreleased structure''' The entry 7k54 is ON HOLD Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A. Description: Mid-translocated +1-fram...

New page

'''Unreleased structure'''

The entry 7k54 is ON HOLD

Authors: Demo, G., Loveland, A.B., Svidritskiy, E., Gamper, H.B., Hou, Y.M., Korostelev, A.A.

Description: Mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS)
[[Category: Unreleased Structures]]
[[Category: Demo, G]]
[[Category: Hou, Y.M]]
[[Category: Loveland, A.B]]
[[Category: Korostelev, A.A]]
[[Category: Gamper, H.B]]
[[Category: Svidritskiy, E]]