OCA at 11:13, 14 June 2023

OCA — Wed, 14 Jun 2023 11:13:49 GMT

←Older revision		Revision as of 11:13, 14 June 2023
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	<StructureSection load='7k6b' size='340' side='right'caption='[[7k6b]]' scene=''>		<StructureSection load='7k6b' size='340' side='right'caption='[[7k6b]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K6B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7k6b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K6B FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k6b OCA], [https://pdbe.org/7k6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k6b RCSB], [https://www.ebi.ac.uk/pdbsum/7k6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k6b ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k6b OCA], [https://pdbe.org/7k6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k6b RCSB], [https://www.ebi.ac.uk/pdbsum/7k6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k6b ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/DYSF_HUMAN DYSF_HUMAN] Miyoshi myopathy;Distal myopathy with anterior tibial onset;Congenital myopathy, Paradas type;Autosomal recessive limb-girdle muscular dystrophy type 2B. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DYSF_HUMAN DYSF_HUMAN] Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The membrane protein dysferlin (DYSF) is important for calcium-activated plasma membrane repair, especially in muscle fibre cells. Nearly 600 mutations in the DYSF gene have been identified that are causative for rare genetic forms of muscular dystrophy. The dysferlin protein consists of seven C2 domains (C2A-C2G, 13%-33% identity) used to recruit calcium ions and traffic accessory proteins and vesicles to injured membrane sites needed to reseal a wound. Amongst these, the C2A is the most prominent facilitating the calcium-sensitive interaction with membrane surfaces. In this work, we determined the calcium-free and calcium-bound structures of the dysferlin C2A domain using NMR spectroscopy and X-ray crystallography. We show that binding two calcium ions to this domain reduces the flexibility of the Ca2+-binding loops in the structure. Furthermore, calcium titration and mutagenesis experiments reveal the tight coupling of these calcium-binding sites whereby the elimination of one site abolishes calcium binding to its partner site. We propose that the electrostatic potential distributed by the flexible, negatively charged calcium-binding loops in the dysferlin C2A domain control first contact with calcium that promotes subsequent binding. Based on these results, we hypothesize that dysferlin uses a 'calcium-catching' mechanism to respond to calcium influx during membrane repair.
		+
		+	Calcium binds and rigidifies the dysferlin C2A domain in a tightly coupled manner.,Wang Y, Tadayon R, Santamaria L, Mercier P, Forristal CJ, Shaw GS Biochem J. 2021 Jan 15;478(1):197-215. doi: 10.1042/BCJ20200773. PMID:33449082<ref>PMID:33449082</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7k6b" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mercier P]]		[[Category: Mercier P]]

OCA at 07:58, 27 January 2021

OCA — Wed, 27 Jan 2021 07:58:13 GMT

←Older revision		Revision as of 07:58, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7k6b is ~~ON HOLD until Paper Publication~~	+	==Solution Structure of the Dysferlin C2A Domain in its Calcium-free State==
-		+	<StructureSection load='7k6b' size='340' side='right'caption='[[7k6b]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7K6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7K6B FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7k6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7k6b OCA], [https://pdbe.org/7k6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7k6b RCSB], [https://www.ebi.ac.uk/pdbsum/7k6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7k6b ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mercier P]]
		+	[[Category: Santamaria L]]
		+	[[Category: Shaw GS]]
		+	[[Category: Wang Y]]

OCA: Protected "7k6b" [edit=sysop:move=sysop]

OCA — Wed, 28 Oct 2020 19:07:05 GMT

Protected "7k6b" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:07, 28 October 2020

OCA: New page: '''Unreleased structure''' The entry 7k6b is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Oct 2020 19:07:03 GMT

New page: '''Unreleased structure''' The entry 7k6b is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7k6b is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

7k6b - Revision history

OCA at 11:13, 14 June 2023

OCA at 07:58, 27 January 2021

OCA: Protected "7k6b" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7k6b is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures