7kil - Revision history

OCA at 15:25, 18 October 2023

OCA — Wed, 18 Oct 2023 15:25:44 GMT

←Older revision		Revision as of 15:25, 18 October 2023
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	<StructureSection load='7kil' size='340' side='right'caption='[[7kil]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='7kil' size='340' side='right'caption='[[7kil]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7kil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.898Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[7kih\|7kih]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Lpin1, Fld ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kil OCA], [https://pdbe.org/7kil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kil RCSB], [https://www.ebi.ac.uk/pdbsum/7kil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kil ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kil OCA], [https://pdbe.org/7kil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kil RCSB], [https://www.ebi.ac.uk/pdbsum/7kil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kil ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/LPIN1_MOUSE LPIN1_MOUSE]] Defects in Lpin1 are the cause of the fatty liver dystrophy phenotype (fld). Fld mutant mices are characterized by neonatal fatty liver and hypertriglyceridemia that resolve at weaning, and neuropathy affecting peripheral nerve in adulthood. Adipose tissue deficiency, glucose intolerance and increased susceptibility to atherosclerosis are associated with this mutation too. Two independent mutant alleles are characterized in this phenotype, fld and fld2j.	+	[https://www.uniprot.org/uniprot/LPIN1_MOUSE LPIN1_MOUSE] Defects in Lpin1 are the cause of the fatty liver dystrophy phenotype (fld). Fld mutant mices are characterized by neonatal fatty liver and hypertriglyceridemia that resolve at weaning, and neuropathy affecting peripheral nerve in adulthood. Adipose tissue deficiency, glucose intolerance and increased susceptibility to atherosclerosis are associated with this mutation too. Two independent mutant alleles are characterized in this phenotype, fld and fld2j.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LPIN1_MOUSE LPIN1_MOUSE]] Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:17158099). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.<ref>PMID:16950137</ref> <ref>PMID:17158099</ref>	+	[https://www.uniprot.org/uniprot/LPIN1_MOUSE LPIN1_MOUSE] Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:17158099). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.<ref>PMID:16950137</ref> <ref>PMID:17158099</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Airola~~, M V~~]]	+	[[Category: Airola MV]]
-	[[Category: Gu, W]]	+	[[Category: Gu W]]
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Lipid binding protein]]~~	+
-	~~[[Category: Lipin]]~~	+
-	~~[[Category: M-lip~~]]	+

OCA at 12:52, 13 October 2021

OCA — Wed, 13 Oct 2021 12:52:55 GMT

←Older revision		Revision as of 12:52, 13 October 2021
Line 1:		Line 1:

	==Crystal structure of the mouse lipin-1 M-Lip domain with zinc==		==Crystal structure of the mouse lipin-1 M-Lip domain with zinc==
-	<StructureSection load='7kil' size='340' side='right'caption='[[7kil]]' scene=''>	+	<StructureSection load='7kil' size='340' side='right'caption='[[7kil]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kil]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kil OCA], [https://pdbe.org/7kil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kil RCSB], [https://www.ebi.ac.uk/pdbsum/7kil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kil ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7kih\|7kih]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Lpin1, Fld ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kil OCA], [https://pdbe.org/7kil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kil RCSB], [https://www.ebi.ac.uk/pdbsum/7kil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kil ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/LPIN1_MOUSE LPIN1_MOUSE]] Defects in Lpin1 are the cause of the fatty liver dystrophy phenotype (fld). Fld mutant mices are characterized by neonatal fatty liver and hypertriglyceridemia that resolve at weaning, and neuropathy affecting peripheral nerve in adulthood. Adipose tissue deficiency, glucose intolerance and increased susceptibility to atherosclerosis are associated with this mutation too. Two independent mutant alleles are characterized in this phenotype, fld and fld2j.
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/LPIN1_MOUSE LPIN1_MOUSE]] Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:17158099). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.<ref>PMID:16950137</ref> <ref>PMID:17158099</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phospholipid synthesis and fat storage as triglycerides are regulated by lipin phosphatidic acid phosphatases (PAPs), whose enzymatic PAP function requires association with cellular membranes. Using hydrogen deuterium exchange mass spectrometry, we find mouse lipin 1 binds membranes through an N-terminal amphipathic helix, the Ig-like domain and HAD phosphatase catalytic core, and a middle lipin (M-Lip) domain that is conserved in mammalian and mammalian-like lipins. Crystal structures of the M-Lip domain reveal a previously unrecognized protein fold that dimerizes. The isolated M-Lip domain binds membranes both in vitro and in cells through conserved basic and hydrophobic residues. Deletion of the M-Lip domain in lipin 1 reduces PAP activity, membrane association, and oligomerization, alters subcellular localization, diminishes acceleration of adipocyte differentiation, but does not affect transcriptional co-activation. This establishes the M-Lip domain as a dimeric protein fold that binds membranes and is critical for full functionality of mammalian lipins.
		+
		+	The middle lipin domain adopts a membrane-binding dimeric protein fold.,Gu W, Gao S, Wang H, Fleming KD, Hoffmann RM, Yang JW, Patel NM, Choi YM, Burke JE, Reue K, Airola MV Nat Commun. 2021 Aug 5;12(1):4718. doi: 10.1038/s41467-021-24929-5. PMID:34354069<ref>PMID:34354069</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kil" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Airola MV]]	+	[[Category: Lk3 transgenic mice]]
-	[[Category: Gu W]]	+	[[Category: Airola, M V]]
		+	[[Category: Gu, W]]
		+	[[Category: Dimer]]
		+	[[Category: Lipid binding protein]]
		+	[[Category: Lipin]]
		+	[[Category: M-lip]]

OCA at 09:57, 7 July 2021

OCA — Wed, 07 Jul 2021 09:57:14 GMT

←Older revision		Revision as of 09:57, 7 July 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7kil ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of the mouse lipin-1 M-Lip domain with zinc==
-		+	<StructureSection load='7kil' size='340' side='right'caption='[[7kil]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KIL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kil OCA], [https://pdbe.org/7kil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kil RCSB], [https://www.ebi.ac.uk/pdbsum/7kil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kil ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Airola MV]]
		+	[[Category: Gu W]]

OCA: Protected "7kil" [edit=sysop:move=sysop]

OCA — Wed, 04 Nov 2020 05:36:48 GMT

Protected "7kil" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:36, 4 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kil is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Nov 2020 05:36:47 GMT

New page: '''Unreleased structure''' The entry 7kil is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7kil is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]