7kk9 - Revision history

OCA at 15:26, 18 October 2023

OCA — Wed, 18 Oct 2023 15:26:37 GMT

←Older revision		Revision as of 15:26, 18 October 2023
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-	====	+	==Fluoride channel Fluc-Ec2 mutant S81A/T82A with bromide==
-	<StructureSection load='7kk9' size='340' side='right'caption='[[7kk9]]' scene=''>	+	<StructureSection load='7kk9' size='340' side='right'caption='[[7kk9]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kk9]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KK9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KK9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kk9 OCA], [https://pdbe.org/7kk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7kk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kk9 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kk9 OCA], [https://pdbe.org/7kk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7kk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kk9 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q6J5N4_ECOLX Q6J5N4_ECOLX] Important for reducing fluoride concentration in the cell, thus reducing its toxicity.[HAMAP-Rule:MF_00454][SAAS:SAAS00096000]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fluc family fluoride channels protect microbes against ambient environmental fluoride by undermining the cytoplasmic accumulation of this toxic halide. These proteins are structurally idiosyncratic, and thus the permeation pathway and mechanism have no analogy in other known ion channels. Although fluoride-binding sites were identified in previous structural studies, it was not evident how these ions access aqueous solution, and the molecular determinants of anion recognition and selectivity have not been elucidated. Using x-ray crystallography, planar bilayer electrophysiology, and liposome-based assays, we identified additional binding sites along the permeation pathway. We used this information to develop an oriented system for planar lipid bilayer electrophysiology and observed anion block at one of these sites, revealing insights into the mechanism of anion recognition. We propose a permeation mechanism involving alternating occupancy of anion-binding sites that are fully assembled only as the substrate approaches.
		+
		+	The fluoride permeation pathway and anion recognition in Fluc family fluoride channels.,McIlwain BC, Gundepudi R, Koff BB, Stockbridge RB Elife. 2021 Jul 12;10:e69482. doi: 10.7554/eLife.69482. PMID:34250906<ref>PMID:34250906</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kk9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: McIlwain BC]]
		+	[[Category: Stockbridge RB]]

OCA at 10:19, 4 August 2021

OCA — Wed, 04 Aug 2021 10:19:08 GMT

←Older revision		Revision as of 10:19, 4 August 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7kk9 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7kk9' size='340' side='right'caption='[[7kk9]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kk9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kk9 OCA], [https://pdbe.org/7kk9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kk9 RCSB], [https://www.ebi.ac.uk/pdbsum/7kk9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kk9 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:14, 28 April 2021

OCA — Wed, 28 Apr 2021 04:14:10 GMT

←Older revision		Revision as of 04:14, 28 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7kk9 is ON HOLD	+	The entry 7kk9 is ON HOLD until Paper Publication

-	Authors: ~~McIlwain, B.C., Stockbridge, R.B.~~	+	Authors:

-	Description: ~~Fluc-Ec2-S81A/T82A~~	+	Description:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Stockbridge, R.B]]
-	[[Category: Mcilwain, B.C]]

OCA at 04:34, 7 April 2021

OCA — Wed, 07 Apr 2021 04:34:37 GMT

←Older revision		Revision as of 04:34, 7 April 2021
Line 3:		Line 3:
	The entry 7kk9 is ON HOLD		The entry 7kk9 is ON HOLD

-	Authors: McIlwain, B.C.	+	Authors: McIlwain, B.C., Stockbridge, R.B.

-	Description: ~~S81T~~/T82A ~~Ec2 in NaBr~~	+	Description: Fluc-Ec2-S81A/T82A
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Stockbridge, R.B]]
	[[Category: Mcilwain, B.C]]		[[Category: Mcilwain, B.C]]

OCA: Protected "7kk9" [edit=sysop:move=sysop]

OCA — Wed, 04 Nov 2020 05:40:40 GMT

Protected "7kk9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:40, 4 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kk9 is ON HOLD Authors: McIlwain, B.C. Description: S81T/T82A Ec2 in NaBr Category: Unreleased Structures Category: Mcilwain, B.C

OCA — Wed, 04 Nov 2020 05:40:39 GMT

New page: '''Unreleased structure''' The entry 7kk9 is ON HOLD Authors: McIlwain, B.C. Description: S81T/T82A Ec2 in NaBr Category: Unreleased Structures Category: Mcilwain, B.C

New page

'''Unreleased structure'''

The entry 7kk9 is ON HOLD

Authors: McIlwain, B.C.

Description: S81T/T82A Ec2 in NaBr
[[Category: Unreleased Structures]]
[[Category: Mcilwain, B.C]]