7kkq - Revision history

OCA at 15:26, 18 October 2023

OCA — Wed, 18 Oct 2023 15:26:56 GMT

←Older revision		Revision as of 15:26, 18 October 2023
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	<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7kkq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kkq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78P:(2R)-2-(7-CARBAMOYL-1H-BENZIMIDAZOL-2-YL)-2-METHYLPYRROLIDINIUM'>78P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.59Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78P:(2R)-2-(7-CARBAMOYL-1H-BENZIMIDAZOL-2-YL)-2-METHYLPYRROLIDINIUM'>78P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [https://pdbe.org/7kkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [https://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [https://pdbe.org/7kkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [https://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TNKS1_HUMAN TNKS1_HUMAN] Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1.<ref>PMID:10988299</ref> <ref>PMID:11739745</ref> <ref>PMID:16076287</ref> <ref>PMID:19759537</ref> <ref>PMID:21478859</ref> <ref>PMID:22864114</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7kkq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7kkq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gajiwala~~, K S~~]]	+	[[Category: Gajiwala KS]]
-	[[Category: Ryan, K]]	+	[[Category: Ryan K]]
-	~~[[Category: Parp1]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 05:33, 28 April 2021

OCA — Wed, 28 Apr 2021 05:33:45 GMT

←Older revision		Revision as of 05:33, 28 April 2021
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	<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]], [[Resolution\|resolution]] 1.59Å' scene=''>		<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7kkq]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kkq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78P:(2R)-2-(7-CARBAMOYL-1H-BENZIMIDAZOL-2-YL)-2-METHYLPYRROLIDINIUM'>78P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78P:(2R)-2-(7-CARBAMOYL-1H-BENZIMIDAZOL-2-YL)-2-METHYLPYRROLIDINIUM'>78P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [~~http~~://pdbe.org/7kkq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [https://pdbe.org/7kkq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [https://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:06, 20 January 2021

OCA — Wed, 20 Jan 2021 06:06:39 GMT

←Older revision		Revision as of 06:06, 20 January 2021
Line 1:		Line 1:

	==Structure of the catalytic domain of tankyrase 1 in complex with veliparib==		==Structure of the catalytic domain of tankyrase 1 in complex with veliparib==
-	<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]]' scene=''>	+	<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]], [[Resolution\|resolution]] 1.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kkq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [http://pdbe.org/7kkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [http://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78P:(2R)-2-(7-CARBAMOYL-1H-BENZIMIDAZOL-2-YL)-2-METHYLPYRROLIDINIUM'>78P</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [http://pdbe.org/7kkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [http://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Poly ADP ribosyltransferases play a critical role in DNA repair and cell death, and PARP1 is a particularly important therapeutic target for the treatment of breast cancer due to its synthetic lethal relationship with BRCA1/2. Numerous PARP1 inhibitors have been developed, and their efficacy in cancer treatment is attributed to both the inhibition of enzymatic activity and their ability to trap PARP1 on to the damaged DNA, which is cytotoxic. Of the clinical PARP inhibitors, talazoparib is the most effective at trapping PARP1 on damaged DNA. Biochemically, talazoparib is also suspected to be a potent inhibitor of PARP5a/b (tankyrase1/2), which is an important regulator of Wnt/beta-catenin pathway. Here we show using competition experiments in cell lysate that, at a clinically relevant concentration, talazoparib can potentially bind and engage tankyrase1. Using surface plasmon resonance, we measured the dissociation constants of talazoparib, olaparib, niraparib and veliparib for their interaction with PARP1 and tankyrase1. The results show that talazoparib has strong affinity for PARP1 as well as uniquely strong affinity for tankyrase1. Finally, we used crystallography and hydrogen deuterium exchange mass spectroscopy to dissect the molecular mechanism of differential selectivity of these PARP1 inhibitors. From these data, we conclude that subtle differences between the ligand binding sites of PARP1 and tankyrase1, differences in the electrostatic nature of the ligands, protein dynamics, and ligand conformational energetics contribute to the different pharmacology of these PARP1 inhibitors. These results will help in the design of drugs to treat Wnt-beta-catenin pathway-related cancers, such as colorectal cancers.
		+
		+	Dissecting the molecular determinants of clinical PARP1 inhibitor selectivity for tankyrase1.,Ryan K, Bolanos B, Smith M, Palde P, Cuenca PD, VanArsdale TL, Niessen S, Zhang L, Behenna D, Ornelas MA, Tran KT, Kaiser S, Lum L, Stewart A, Gajiwala KS J Biol Chem. 2020 Dec 24. pii: RA120.016573. doi: 10.1074/jbc.RA120.016573. PMID:33361107<ref>PMID:33361107</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kkq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gajiwala KS]]	+	[[Category: Gajiwala, K S]]
-	[[Category: Ryan K]]	+	[[Category: Ryan, K]]
		+	[[Category: Parp1]]
		+	[[Category: Transferase]]

OCA at 08:58, 6 January 2021

OCA — Wed, 06 Jan 2021 08:58:00 GMT

←Older revision		Revision as of 08:58, 6 January 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 7kkq is ON HOLD until Paper Publication~~	+	==Structure of the catalytic domain of tankyrase 1 in complex with veliparib==
-		+	<StructureSection load='7kkq' size='340' side='right'caption='[[7kkq]]' scene=''>
-	~~Authors: Gajiwala, K.S., Ryan, K.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KKQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7KKQ FirstGlance]. <br>
-	~~Description:~~ Structure of the catalytic domain of tankyrase 1 in complex with veliparib	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7kkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kkq OCA], [http://pdbe.org/7kkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7kkq RCSB], [http://www.ebi.ac.uk/pdbsum/7kkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7kkq ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Ryan, K~~]]	+	__TOC__
-	[[Category: ~~Gajiwala,~~ K.S]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gajiwala KS]]
		+	[[Category: Ryan K]]

OCA: Protected "7kkq" [edit=sysop:move=sysop]

OCA — Wed, 04 Nov 2020 05:43:08 GMT

Protected "7kkq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 4 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kkq is ON HOLD until Paper Publication Authors: Gajiwala, K.S., Ryan, K. Description: Structure of the catalytic domain of tankyrase 1 in complex ...

OCA — Wed, 04 Nov 2020 05:43:06 GMT

New page: '''Unreleased structure''' The entry 7kkq is ON HOLD until Paper Publication Authors: Gajiwala, K.S., Ryan, K. Description: Structure of the catalytic domain of tankyrase 1 in complex ...

New page

'''Unreleased structure'''

The entry 7kkq is ON HOLD until Paper Publication

Authors: Gajiwala, K.S., Ryan, K.

Description: Structure of the catalytic domain of tankyrase 1 in complex with veliparib
[[Category: Unreleased Structures]]
[[Category: Ryan, K]]
[[Category: Gajiwala, K.S]]