7kmv - Revision history

OCA at 15:28, 18 October 2023

OCA — Wed, 18 Oct 2023 15:28:30 GMT

←Older revision		Revision as of 15:28, 18 October 2023
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	==Structure of Malaysian Banana Lectin F84T==		==Structure of Malaysian Banana Lectin F84T==
-	<StructureSection load='7kmv' size='340' side='right'caption='[[7kmv]]' scene=''>	+	<StructureSection load='7kmv' size='340' side='right'caption='[[7kmv]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KMV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kmv]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Musa_acuminata Musa acuminata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KMV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kmv OCA], [https://pdbe.org/7kmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kmv RCSB], [https://www.ebi.ac.uk/pdbsum/7kmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kmv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kmv OCA], [https://pdbe.org/7kmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kmv RCSB], [https://www.ebi.ac.uk/pdbsum/7kmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kmv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lectins, carbohydrate-binding proteins, have been regarded as potential antiviral agents, as some can bind glycans on viral surface glycoproteins and inactivate their functions. However, clinical development of lectins has been stalled by the mitogenicity of many of these proteins, which is the ability to stimulate deleterious proliferation, especially of immune cells. We previously demonstrated that the mitogenic and antiviral activities of a lectin (banana lectin, BanLec) can be separated via a single amino acid mutation, histidine to threonine at position 84 (H84T), within the third Greek key. The resulting lectin, H84T BanLec, is virtually non-mitogenic but retains antiviral activity. Decreased mitogenicity was associated with disruption of pi-pi stacking between two aromatic amino acids. To examine whether we could provide further proof-of-principle of the ability to separate these two distinct lectin functions, we identified another lectin, Malaysian banana lectin (Malay BanLec), with similar structural features as BanLec, including pi-pi stacking, but with only 63% amino acid identity, and showed that it is both mitogenic and potently antiviral. We then engineered an F84T mutation expected to disrupt pi-pi stacking, analogous to H84T. As predicted, F84T Malay BanLec (F84T) was less mitogenic than wild type. However, F84T maintained strong antiviral activity and inhibited replication of HIV, Ebola, and other viruses. The F84T mutation disrupted pi-pi stacking without disrupting the overall lectin structure. These findings show that pi-pi stacking in the third Greek key is a conserved mitogenic motif in these two jacalin-related lectins BanLec and Malay BanLec, and further highlight the potential to rationally engineer antiviral lectins for therapeutic purposes.
		+
		+	Targeted disruption of pi-pi stacking in Malaysian banana lectin reduces mitogenicity while preserving antiviral activity.,Coves-Datson EM, King SR, Legendre M, Swanson MD, Gupta A, Claes S, Meagher JL, Boonen A, Zhang L, Kalveram B, Raglow Z, Freiberg AN, Prichard M, Stuckey JA, Schols D, Markovitz DM Sci Rep. 2021 Jan 12;11(1):656. doi: 10.1038/s41598-020-80577-7. PMID:33436903<ref>PMID:33436903</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kmv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Musa acuminata]]
	[[Category: Meagher JL]]		[[Category: Meagher JL]]
	[[Category: Stuckey JA]]		[[Category: Stuckey JA]]

OCA at 07:58, 27 January 2021

OCA — Wed, 27 Jan 2021 07:58:37 GMT

←Older revision		Revision as of 07:58, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7kmv ~~is ON HOLD until Paper Publication~~	+	==Structure of Malaysian Banana Lectin F84T==
-		+	<StructureSection load='7kmv' size='340' side='right'caption='[[7kmv]]' scene=''>
-	~~Authors~~: ~~Meagher~~, J.L., ~~Stuckey~~, J.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KMV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KMV FirstGlance]. <br>
-	~~Description: Structure of Malaysian Banana Lectin F84T~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kmv OCA], [https://pdbe.org/7kmv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kmv RCSB], [https://www.ebi.ac.uk/pdbsum/7kmv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kmv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Stuckey, J.A~~]]	+	__TOC__
-	[[Category: ~~Meagher, J.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Meagher JL]]
		+	[[Category: Stuckey JA]]

OCA at 05:13, 9 December 2020

OCA — Wed, 09 Dec 2020 05:13:25 GMT

←Older revision		Revision as of 05:13, 9 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7kmv is ON HOLD	+	The entry 7kmv is ON HOLD until Paper Publication

	Authors: Meagher, J.L., Stuckey, J.A.		Authors: Meagher, J.L., Stuckey, J.A.
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	Description: Structure of Malaysian Banana Lectin F84T		Description: Structure of Malaysian Banana Lectin F84T
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Meagher, J.L]]
	[[Category: Stuckey, J.A]]		[[Category: Stuckey, J.A]]
		+	[[Category: Meagher, J.L]]

OCA: Protected "7kmv" [edit=sysop:move=sysop]

OCA — Wed, 11 Nov 2020 06:35:32 GMT

Protected "7kmv" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:35, 11 November 2020

OCA: New page: '''Unreleased structure''' The entry 7kmv is ON HOLD Authors: Meagher, J.L., Stuckey, J.A. Description: Structure of Malaysian Banana Lectin F84T Category: Unreleased Structures [[...

OCA — Wed, 11 Nov 2020 06:35:30 GMT

New page: '''Unreleased structure''' The entry 7kmv is ON HOLD Authors: Meagher, J.L., Stuckey, J.A. Description: Structure of Malaysian Banana Lectin F84T Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 7kmv is ON HOLD

Authors: Meagher, J.L., Stuckey, J.A.

Description: Structure of Malaysian Banana Lectin F84T
[[Category: Unreleased Structures]]
[[Category: Meagher, J.L]]
[[Category: Stuckey, J.A]]