OCA at 19:36, 29 May 2024

OCA — Wed, 29 May 2024 19:36:30 GMT

←Older revision		Revision as of 19:36, 29 May 2024
Line 1:		Line 1:

-	====	+	==Staphylococcus aureus 30S ribosomal subunit in presence of spermidine==
-	<StructureSection load='7kwg' size='340' side='right'caption='[[7kwg]]' scene=''>	+	<StructureSection load='7kwg' size='340' side='right'caption='[[7kwg]], [[Resolution\|resolution]] 3.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7kwg]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KWG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwg OCA], [https://pdbe.org/7kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwg RCSB], [https://www.ebi.ac.uk/pdbsum/7kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.75Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwg OCA], [https://pdbe.org/7kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwg RCSB], [https://www.ebi.ac.uk/pdbsum/7kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RS20_STAA8 RS20_STAA8] Binds directly to 16S ribosomal RNA.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cryo-electron microscopy is now used as a method of choice in structural biology for studying protein synthesis, a process mediated by the ribosome machinery. In order to achieve high-resolution structures using this approach, one needs to obtain homogeneous and stable samples, which requires optimization of ribosome purification in a species-dependent manner. This is especially critical for the bacterial small ribosomal subunit that tends to be unstable in the absence of ligands. Here, we report a protocol for purification of stable 30 S from the Gram-positive bacterium Staphylococcus aureus and its cryo-EM structures: in presence of spermidine at a resolution ranging between 3.4 and 3.6 A and in its absence at 5.3 A. Using biochemical characterization and cryo-EM, we demonstrate the importance of spermidine for stabilization of the 30 S via preserving favorable conformation of the helix 44.
		+
		+	Stabilization of Ribosomal RNA of the Small Subunit by Spermidine in Staphylococcus aureus.,Belinite M, Khusainov I, Soufari H, Marzi S, Romby P, Yusupov M, Hashem Y Front Mol Biosci. 2021 Nov 18;8:738752. doi: 10.3389/fmolb.2021.738752. , eCollection 2021. PMID:34869582<ref>PMID:34869582</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7kwg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribosome 3D structures\|Ribosome 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
		+	[[Category: Belinite M]]
		+	[[Category: Hashem Y]]
		+	[[Category: Khusainov I]]
		+	[[Category: Marzi S]]
		+	[[Category: Romby P]]
		+	[[Category: Yusupov M]]

OCA at 07:58, 27 January 2021

OCA — Wed, 27 Jan 2021 07:58:55 GMT

←Older revision		Revision as of 07:58, 27 January 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7kwg ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7kwg' size='340' side='right'caption='[[7kwg]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kwg OCA], [https://pdbe.org/7kwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kwg RCSB], [https://www.ebi.ac.uk/pdbsum/7kwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kwg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA: Protected "7kwg" [edit=sysop:move=sysop]

OCA — Wed, 09 Dec 2020 05:16:52 GMT

Protected "7kwg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 9 December 2020

OCA: New page: '''Unreleased structure''' The entry 7kwg is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 Dec 2020 05:16:51 GMT

New page: '''Unreleased structure''' The entry 7kwg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7kwg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7kwg - Revision history

OCA at 19:36, 29 May 2024

OCA at 07:58, 27 January 2021

OCA: Protected "7kwg" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7kwg is ON HOLD Authors: Description: Category: Unreleased Structures